Patents by Inventor Adi Barzel
Adi Barzel has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220204995Abstract: Compositions and methods are provided for integrating one or more genes of interest into cellular DNA without substantially disrupting the expression of the gene at the locus of integration, i.e., the target locus. These compositions and methods are useful in any in vitro or in vivo application in which it is desirable to express a gene of interest in the same spatially and temporally restricted pattern as that of a gene at a target locus while maintaining the expression of the gene at the target locus, for example, to treat disease, in the production of genetically modified organisms in agriculture, in the large scale production of proteins by cells for therapeutic, diagnostic, or research purposes, in the induction of iPS cells for therapeutic, diagnostic, or research purposes, in biological research, etc. Reagents, devices and kits thereof that find use in practicing the subject methods are also provided.Type: ApplicationFiled: August 26, 2021Publication date: June 30, 2022Inventors: Mark A. Kay, Matthew Porteus, Jenny Barker, Josh Checketts, Richard Voit, Adi Barzel
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Publication number: 20210277419Abstract: Methods and compositions are provided for editing the genome of a cell without the use of an exogenously supplied nuclease. Aspects of the methods include contacting a cell with a targeting vector comprising nucleic acid sequence to be integrated into the target locus, where the cell is not also contacted with a nuclease. In addition, reagents, devices and kits thereof that find use in practicing the subject methods are provided.Type: ApplicationFiled: May 17, 2021Publication date: September 9, 2021Inventors: Adi Barzel, Mark A. Kay
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Publication number: 20210269771Abstract: The present invention relates to methods and compositions for engineering B cells to express transgenic B cell receptor (BCR) for antigen-induced antibody secretion, compositions, methods and uses thereof in immunotherapy.Type: ApplicationFiled: March 12, 2021Publication date: September 2, 2021Inventors: Adi BARZEL, Alessio D. NAHMAD, Tal AKRIV, Miriam FRIED, Iris DOTAN, Daniel NATAF
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Publication number: 20210214752Abstract: Compositions and methods are provided for integrating one or more genes of interest into cellular DNA without substantially disrupting the expression of the gene at the locus of integration, i.e., the target locus. These compositions and methods are useful in any in vitro or in vivo application in which it is desirable to express a gene of interest in the same spatially and temporally restricted pattern as that of a gene at a target locus while maintaining the expression of the gene at the target locus, for example, to treat disease, in the production of genetically modified organisms in agriculture, in the large scale production of proteins by cells for therapeutic, diagnostic, or research purposes, in the induction of PS cells for therapeutic, diagnostic, or research purposes, in biological research, etc. Reagents, devices and kits thereof that find use in practicing the subject methods are also provided.Type: ApplicationFiled: April 7, 2020Publication date: July 15, 2021Inventors: Mark A. Kay, Matthew Porteus, Jenny Barker, Josh Checketts, Richard Voit, Adi Barzel
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Publication number: 20210017539Abstract: Methods and compositions are provided for editing the genome of a cell without the use of an exogenously supplied nuclease. Aspects of the methods include contacting a cell with a targeting vector comprising nucleic acid sequence to be integrated into the target locus, where the cell is not also contacted with a nuclease. In addition, reagents, devices and kits thereof that find use in practicing the subject methods are provided.Type: ApplicationFiled: February 26, 2020Publication date: January 21, 2021Inventors: Adi Barzel, Mark A. Kay
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Publication number: 20200131540Abstract: The present invention relates to methods for targeted insertion of at least one nucleic acid sequence/s of interest into a target genomic locus of a mammalian cell. More specifically, the methods of the invention are based on using nucleic acid cassettes comprising the nucleic acid sequence/s of interest and at least one recognition signal sequence (RSS), for insertion of the nucleic acid sequence of interest into the target genomic locus that is mediated by RAG-catalyzed recombination. The invention further provides cassettes, vectors and vehicles and cells comprising said cassettes, compositions and uses thereof in immunotherapy.Type: ApplicationFiled: April 18, 2018Publication date: April 30, 2020Inventors: Adi BARZEL, Iris DOTAN, Carmel PUNDAK-MINTZ, Daniel NATAF, Miriam FRIED, Natalia GRITSENKO
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Patent number: 10612041Abstract: Methods and compositions are provided for editing the genome of a cell without the use of an exogenously supplied nuclease. Aspects of the methods include contacting a cell with a targeting vector comprising nucleic acid sequence to be integrated into the target locus, where the cell is not also contacted with a nuclease. In addition, reagents, devices and kits thereof that find use in practicing the subject methods are provided.Type: GrantFiled: March 19, 2015Date of Patent: April 7, 2020Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Adi Barzel, Mark A. Kay
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Publication number: 20170233765Abstract: Compositions and methods are provided for integrating one or more genes of interest into cellular DNA without substantially disrupting the expression of the gene at the locus of integration, i.e., the target locus. These compositions and methods are useful in any in vitro or in vivo application in which it is desirable to express a gene of interest in the same spatially and temporally restricted pattern as that of a gene at a target locus while maintaining the expression of the gene at the target locus, for example, to treat disease, in the production of genetically modified organisms in agriculture, in the large scale production of proteins by cells for therapeutic, diagnostic, or research purposes, in the induction of iPS cells for therapeutic, diagnostic, or research purposes, in biological research, etc. Reagents, devices and kits thereof that find use in practicing the subject methods are also provided.Type: ApplicationFiled: March 13, 2017Publication date: August 17, 2017Inventors: Mark A. Kay, Matthew Porteus, Jenny Barker, Josh Checketts, Richard Voit, Adi Barzel
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Publication number: 20170088856Abstract: Methods and compositions are provided for editing the genome of a cell without the use of an exogenously supplied nuclease. Aspects of the methods include contacting a cell with a targeting vector comprising nucleic acid sequence to be integrated into the target locus, where the cell is not also contacted with a nuclease. In addition, reagents, devices and kits thereof that find use in practicing the subject methods are provided.Type: ApplicationFiled: March 19, 2015Publication date: March 30, 2017Inventors: Adi Barzel, Mark A. Kay
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Publication number: 20150196917Abstract: A method and apparatus for synthesizing nucleic acid (NA) by sequential addition of nucleotide triphosphates to a single stranded NA molecule (growing-chain) is described. The growing-chain is annealed to an oligonucleotide, wherein the 3? end of the oligonucleotide is complementary to the 3? end of the growing-chain and wherein the protruding 5? end of the oligonucleotide has the correct sequence to serve as a template for the growing-chain to produce a NA product. The growing-chain is then brought into contact with a polymerase in the presence of one or more nucleotide triphosphates under conditions permitting the polymerase to catalyze addition of one or more nucleotide triphosphates to the 3? end of the growing-chain using the annealed oligonucleotide as a template. After polymerization has occurred, the annealed growing-chain and oligonucleotide are denatured so that the steps can be repeated until the NA product of desired length is synthesized.Type: ApplicationFiled: January 26, 2015Publication date: July 16, 2015Applicant: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITYInventors: Mark A. Kay, Adi Barzel
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Publication number: 20130280222Abstract: Compositions and methods are provided for integrating one or more genes of interest into cellular DNA without substantially disrupting the expression of the gene at the locus of integration, i.e., the target locus. These compositions and methods are useful in any in vitro or in vivo application in which it is desirable to express a gene of interest in the same spatially and temporally restricted pattern as that of a gene at a target locus while maintaining the expression of the gene at the target locus, for example, to treat disease, in the production of genetically modified organisms in agriculture, in the large scale production of proteins by cells for therapeutic, diagnostic, or research purposes, in the induction of iPS cells for therapeutic, diagnostic, or research purposes, in biological research, etc. Reagents, devices and kits thereof that find use in practicing the subject methods are also provided.Type: ApplicationFiled: March 15, 2013Publication date: October 24, 2013Inventors: Mark A. Kay, Matthew Porteus, Jenny Barker, Josh Checketts, Richard Voit, Adi Barzel
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Patent number: 8566040Abstract: A computer implemented method for generating nucleotide sequences containing candidate homing endonuclease genes (HEGs). A search is performed in a database stored on a storage medium of nucleotide sequences for amino acid sequences having a subsequence having a homology level with the translation of a subsequence of one or more predetermined HEGs. For each amino acid sequence generated by the search, one or more nucleotide sequences are retrieved encoding the amino acid sequence. The results of this search used in a second search of a database stored on a storage medium to generate the HEG containing sequences.Type: GrantFiled: February 12, 2009Date of Patent: October 22, 2013Assignee: Ramot at Tel-Aviv University LtdInventors: Adi Barzel, Eyal Privman, David Burstein, Uri Gophna, Martin Kupiec, Tal Pupko
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Publication number: 20110064717Abstract: The invention provides pharmaceutical composition having as an active ingredient either a homing endonuclease (HE) capable of cleaving a non-native target nucleotide sequence in a genome or a nucleotide sequence encoding for a HE capable of cleaving a target site in a non-native genome. The invention also provides uses for such HEs, and methods of treatment utilizing such HEs. The HE may be, for example, any one of the HEs PI-SceI, POLB HE, PRP8 HE, or Nostoc species PCC7120 HE.Type: ApplicationFiled: August 20, 2010Publication date: March 17, 2011Applicant: Ramot at Tel-Aviv University Ltd.Inventors: Adi Barzel, Eyal Privman, Michael Pe'eri, Martin Kupiec, Tal Pupko
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Publication number: 20100317724Abstract: A computer implemented method for generating nucleotide sequences containing candidate homing endonuclease genes (HEGs). A search is performed in a database stored on a storage medium of nucleotide sequences for amino acid sequences having a subsequence having a homology level with the translation of a subsequence of one or more predetermined HEGs. For each amino acid sequence generated by the search, one or more nucleotide sequences are retrieved encoding the amino acid sequence. The results of this search used in a second search of a database stored on a storage medium to generate the HEG containing sequences.Type: ApplicationFiled: February 12, 2009Publication date: December 16, 2010Applicant: RAMOT AT TEL-AVIV UNIVERSITY LTD.Inventors: Adi Barzel, Eyal Privman, David Burstein, Uri Gophna, Martin Kupiec, Tal Pupko