Patents by Inventor Aditya Rajagopal

Aditya Rajagopal has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20220127664
    Abstract: The present disclosure provides methods and compositions for detection of multiple nucleic acid targets in a single optical channel in a digital assay. In some cases, three or more nucleic acid targets may be detected in a single channel. Nucleic acid targets may be partitioned, amplified, used to generate signals, where each signal corresponds to a unique combination of nucleic acid targets in a partition.
    Type: Application
    Filed: January 7, 2022
    Publication date: April 28, 2022
    Inventor: Aditya RAJAGOPAL
  • Publication number: 20220002788
    Abstract: A method of identifying a first target nucleic acid comprising, providing a sample comprising the first target nucleic acid, providing a first set of paired oligonucleotides with complementarity to the first target nucleic acid, the first set of paired oligonucleotides comprising a first ratio of (a) first competitive oligonucleotides to (b) first signal oligonucleotides comprising a signal tag, wherein the competitive oligonucleotides compete with the signal oligonucleotides for binding to the first target nucleic acid, amplifying the first target nucleic acid with the polymerase chain reaction, thereby degrading the first signal oligonucleotide and permitting generation of a first signal, generating the first signal, measuring intensity of the first signal, and correlating the intensity of the first signal to the first ratio, thereby identifying the first target nucleic acid.
    Type: Application
    Filed: September 21, 2021
    Publication date: January 6, 2022
    Inventors: Lucien A.E. JACKY, Aditya RAJAGOPAL, Karen L. MENGE, Gregory GOSCH
  • Publication number: 20210363581
    Abstract: FRET-based analytes detection and related methods and systems are described where a pair of FRET labeled primers and/or oligonucleotides are used that are specific for target sequences located at a distance up to four time the Förster distance of the FRET chromophores presented on the FRET labeled primers and/or oligonucleotides one with respect to the other in one or more polynucleotide analyte; in particular the pair of FRET labeled primers and/or oligonucleotides is combined with a sample and subjected to one or more polynucleotide amplification reactions before measuring FRET signals from at least one FRET chromophore.
    Type: Application
    Filed: December 17, 2020
    Publication date: November 25, 2021
    Applicant: California Institute of Technology
    Inventors: Emil P. Kartalov, Aditya Rajagopal, Axel Scherer, Mark D. Goldberg
  • Publication number: 20210292817
    Abstract: The present disclosure provides methods, systems, and compositions of quantifying one or more analytes in a single sample volume without immobilization, separation, mass spectrometry, or melting curve analysis. Methods may comprise polymerase chain reaction and signal generation to quantify the analytes.
    Type: Application
    Filed: July 10, 2019
    Publication date: September 23, 2021
    Inventors: Aditya RAJAGOPAL, Christopher MACDONALD
  • Publication number: 20210262083
    Abstract: Methods for regulating and continuously monitoring a chemical synthesis reaction using micro-objects and electro-magnetic radiation include introducing micro-objects to a reaction mixture, determining a plasmon resonance of the micro-object based on a characteristic of the micro-object, and applying electro-magnetic radiation that is wavelength-matched to the plasmon resonance of the micro-object.
    Type: Application
    Filed: February 24, 2021
    Publication date: August 26, 2021
    Applicant: California Institute of Technology
    Inventor: Aditya Rajagopal
  • Publication number: 20210254147
    Abstract: The present disclosure provides methods and compositions for multiplex quantitation. In some aspects, methods and compositions are provided for quantitation of nucleic acid targets from a biological sample. The disclosed methods may be useful in identifying or detecting genetic abnormalities from a subject.
    Type: Application
    Filed: April 16, 2019
    Publication date: August 19, 2021
    Inventors: Christopher MACDONALD, Aditya RAJAGOPAL, Dominic YURK
  • Publication number: 20210208071
    Abstract: The present disclosure provides methods and compositions to “watermark” chemical compounds. The disclosed methods provide methods whereby the unique watermark allows identification of substantially similar chemical reactions in a single qPCR reaction mixture. Further, the disclosure provides methods whereby the unique watermark allows for identification of reaction mixtures.
    Type: Application
    Filed: June 3, 2019
    Publication date: July 8, 2021
    Inventors: Aditya RAJAGOPAL, Alexander DICKINSON, Forest BASKETT
  • Publication number: 20210189473
    Abstract: The present disclosure provides methods and compositions for identifying and discriminating between one or more target nucleic acids within a sample. The disclosed methods may be useful for detecting single nucleotide polymorphisms (SNPs). The disclosed methods may be useful in identifying or detecting the presence or absence of a nucleotide mutation. The use of mutation-specific oligonucleotide probes with universal tails enables specific detection of a SNP target present in a sample.
    Type: Application
    Filed: September 6, 2018
    Publication date: June 24, 2021
    Inventors: Aditya RAJAGOPAL, Lucien JACKY, Karen L. MENGE
  • Publication number: 20210169441
    Abstract: An enhanced stethoscope device and method for operating the enhanced stethoscope are provided. The enhanced stethoscope device generally operates by providing stethoscope sensors, ultrasonic sensors, and other sensors to obtain a series of measurements about a subject. The series of measurements may be correlated, such as by machine learning, to extract clinically relevant information. Also described are systems and methods for ultrasonic beamsteering by interference of an audio signal with an ultrasonic signal.
    Type: Application
    Filed: July 13, 2020
    Publication date: June 10, 2021
    Inventors: Aditya RAJAGOPAL, Alaina Ann Brinley RAJAGOPAL
  • Publication number: 20210098076
    Abstract: This disclosure provides methods, systems, compositions, and kits for the multiplexed detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, systems, compositions, and kits wherein multiple analytes may be detected in a single sample volume by acquiring a cumulative measurement or measurements of at least one quantifiable component of a signal. In some cases, additional components of a signal, or additional signals (or components thereof) are also quantified. Each signal or component of a signal may be used to construct a coding scheme which can then be used to determine the presence or absence of any analyte.
    Type: Application
    Filed: May 1, 2020
    Publication date: April 1, 2021
    Inventors: Emil P. Kartalov, Aditya Rajagopal, Axel Scherer
  • Publication number: 20210087607
    Abstract: The present disclosure provides methods and compositions for nucleic acid detection. Nucleic acids may be derived from any source including, for example, viruses, bacterial cells, and eukaryotic cells. The methods of the present disclosure may be used to detect the presence of at least one member of a plurality of nucleic acids in a sample. The methods of the present disclosure may be used to detect the presence of both a first and second member of a plurality of nucleic acids in a sample. Nucleic acids may be detected by the generation of one or more signals.
    Type: Application
    Filed: May 7, 2020
    Publication date: March 25, 2021
    Inventors: Aditya RAJAGOPAL, Christopher MACDONALD
  • Publication number: 20210076954
    Abstract: Some implementations of the disclosure describe a blood pressure measurement apparatus and method that enable continuous, non-invasive blood pressure measurement using sound and ultrasound transducers. In one implementation, a blood pressure measurement device includes: a first transducer configured to emit multiple soundwaves having multiple frequencies, the soundwaves configured to cause a blood vessel of a subject to vibrate; a second transducer configured to capture one or more ultrasound images of the blood vessel; and a processing device configured to: determine, based on the one or more ultrasound images, a wall thickness, a radius, and a resonant frequency of the blood vessel; and calculate, based on the wall thickness, the radius, and the resonant frequency, a blood pressure of the subject.
    Type: Application
    Filed: September 14, 2020
    Publication date: March 18, 2021
    Applicant: California Institute of Technology
    Inventors: Aditya Rajagopal, Dominic Yurk, Yaser Abu-Mostafa, Alaina Ann Brinley Rajagopal
  • Publication number: 20210012860
    Abstract: This disclosure provides methods, systems, compositions, and kits for the multiplexed detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, systems, compositions, and kits wherein multiple analytes may be detected in a single sample volume by acquiring a cumulative measurement or measurements of at least one quantifiable component of a signal. In some cases, additional components of a signal, or additional signals (or components thereof) are also quantified. Each signal or component of a signal may be used to construct a coding scheme which can then be used to determine the presence or absence of any analyte.
    Type: Application
    Filed: July 23, 2020
    Publication date: January 14, 2021
    Inventors: Emil P. Kartalov, Aditya Rajagopal, Axel Scherer
  • Patent number: 10889863
    Abstract: FRET-based analytes detection and related methods and systems are described where a pair of FRET labeled primers and/or oligonucleotides are used that are specific for target sequences located at a distance up to four time the Förster distance of the FRET chromophores presented on the FRET labeled primers and/or oligonucleotides one with respect to the other in one or more polynucleotide analyte; in particular the pair of FRET labeled primers and/or oligonucleotides is combined with a sample and subjected to one or more polynucleotide amplification reactions before measuring FRET signals from at least one FRET chromophore.
    Type: Grant
    Filed: August 13, 2018
    Date of Patent: January 12, 2021
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Emil P. Kartalov, Aditya Rajagopal, Axel Scherer, Mark D. Goldberg
  • Publication number: 20210002732
    Abstract: Medical systems for detecting a genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, and the first primer and the second primer are specific for the polynucleotide analyte. The primers are configured to amplify the polynucleotide analyte having the genetic variation and a corresponding polynucleotide analyte lacking the generic variation. There is a detectable difference between a change in signal generated by the fluorophore and quencher, and measured by a sensor of the medical system, when using the first and second primers to amplify the polynucleotide analyte with the genetic variation, and a change in signal generated by the fluorophore and quencher, and measured by the sensor of the medical system, when using the first and second primers to amplify the corresponding polynucleotide analyte lacking the genetic variation.
    Type: Application
    Filed: March 23, 2020
    Publication date: January 7, 2021
    Inventors: Aditya Rajagopal, Mark D. Goldberg, Erika F. Garcia, Xiomara L. Madero, Thomas A. Tombrello, Axel Scherer
  • Publication number: 20200399677
    Abstract: Methods for differentially quantifying nucleic acids in a biological sample using differentially methylated loci include forming a digested biological sample in a solution of a methylation-sensitive restriction enzyme to cleave an unmethylated group of nucleic acids, forming a reaction mixture, amplifying the reaction mixture to generate a first signal from the a group of detection probes and a second signal from a second group of detection probes, determining a ratio comprising a first value derived from the first signal to a second value derived from the second signal, and identifying the proportion of nucleic acid in the biological sample originating from the first plurality of nucleic acids.
    Type: Application
    Filed: June 24, 2020
    Publication date: December 24, 2020
    Inventors: Christopher MacDonald, Lucien Jacky, Aditya Rajagopal
  • Patent number: 10770170
    Abstract: This disclosure provides methods, systems, compositions, and kits for the multiplexed detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, systems, compositions, and kits wherein multiple analytes may be detected in a single sample volume by acquiring a cumulative measurement or measurements of at least one quantifiable component of a signal. In some cases, additional components of a signal, or additional signals (or components thereof) are also quantified. Each signal or component of a signal may be used to construct a coding scheme which can then be used to determine the presence or absence of any analyte.
    Type: Grant
    Filed: March 7, 2018
    Date of Patent: September 8, 2020
    Assignee: California Institute of Technology
    Inventors: Emil P. Kartalov, Aditya Rajagopal, Axel Scherer
  • Patent number: 10751025
    Abstract: An enhanced stethoscope device and method for operating the enhanced stethoscope are provided. The enhanced stethoscope device generally operates by providing stethoscope sensors, ultrasonic sensors, and other sensors to obtain a series of measurements about a subject. The series of measurements may be correlated, such as by machine learning, to extract clinically relevant information. Also described are systems and methods for ultrasonic beamsteering by interference of an audio signal with an ultrasonic signal.
    Type: Grant
    Filed: November 20, 2019
    Date of Patent: August 25, 2020
    Assignee: California Institute of Technology
    Inventors: Aditya Rajagopal, Alaina Ann Brinley Rajagopal
  • Publication number: 20200138400
    Abstract: An enhanced stethoscope device and method for operating the enhanced stethoscope are provided. The enhanced stethoscope device generally operates by providing stethoscope sensors, ultrasonic sensors, and other sensors to obtain a series of measurements about a subject. The series of measurements may be correlated, such as by machine learning, to extract clinically relevant information. Also described are systems and methods for ultrasonic beamsteering by interference of an audio signal with an ultrasonic signal.
    Type: Application
    Filed: November 20, 2019
    Publication date: May 7, 2020
    Inventors: Aditya Rajagopal, Alaina Ann Brinley Rajagopal
  • Patent number: 10597737
    Abstract: Methods and kits for detecting a genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, and the first primer and the second primer are specific for the polynucleotide analyte. At least one of the primers is configured to hybridize to a region of the polynucleotide analyte encoding the genetic variation. The primers are configured to amplify the polynucleotide analyte having the genetic variation and a corresponding polynucleotide analyte lacking the generic variation. There is a detectable difference between a change in signal generated by the fluorophore and quencher when using the first and second primers to amplify the polynucleotide analyte with the genetic variation, and a change in signal generated by the fluorophore and quencher when using the first and second primers to amplify the corresponding polynucleotide analyte lacking the genetic variation.
    Type: Grant
    Filed: August 22, 2018
    Date of Patent: March 24, 2020
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Aditya Rajagopal, Mark D. Goldberg, Erika F. Garcia, Xiomara L. Madero, Thomas A. Tombrello, Axel Scherer