Abstract: Provided are methods, kits, and compositions for preparing platelet compositions suitable for infusion, including improved methods, compositions, and kits for pathogen inactivation of an apheresis-derived preparation of platelets.

Abstract: A circuit in an image-capturing device is provided. The circuit includes a light source controller configured to provide a signal to a light source when a cartridge mount has received, inside a dark chamber, a test cartridge. The circuit also includes a sensor array controller configured to activate at least one pixel in a sensor array when the light source is activated, and to receive a signal from the at least one pixel, the signal indicative of an optical intensity of a light emitted from the test cartridge. The circuit also includes a processor, configured to form a transmittable file with the signal from the at least one pixel, and a radio-frequency antenna configured to transmit the transmittable file to an external processor.

Abstract: A method for capturing images of a liquid immunoassay to determine a target analyte concentration in a fluid sample includes collecting, with a camera, multiple images of a fluid flow over an assay, the assay including a substrate having a surface that is functionalized with Abs, and the fluid flow including multiple particles, which become bound to functionalized surface when a target analyte is present, combining the images of the fluid flow to obtain a processed image, identifying, in the processed image, one or more particles bound to the substrate, and determining a target analyte concentration in the fluid flow based on a number of particles bound to the substrate. A system including a memory with instructions and a processor to execute the instructions and cause the system to perform the above method is also provided.

Abstract: Disclosed are various embodiments of a device comprising a synthetic polymeric substrate having a high quality finish upper surface, the upper surface having at least a bilayer coating comprising a first, reflective layer and a second, transparent layer. Also disclosed are kits containing embodiments of the disclosed device and detectable particles. Also disclosed are various embodiments of a method of using the disclosed device and various embodiments of a method of using the disclosed kit.

Abstract: Disclosed are various embodiments of a device comprising a synthetic polymeric substrate having a high quality finish upper surface, the upper surface having at least a bilayer coating comprising a first, reflective layer and a second, transparent layer. Also disclosed are kits containing embodiments of the disclosed device and detectable particles. Also disclosed are various embodiments of a method of using the disclosed device and various embodiments of a method of using the disclosed kit.

Abstract: A method to assess a subject diagnosis is provided. The method includes receiving an image from an image-capturing device, the image comprising an area of interest in a test cartridge, and finding a border of the area of interest of the test cartridge and applying a geometrical transformation on an area delimited by the border of the test cartridge to bring the image of the area of interest in the test cartridge to a selected size and a selected shape. The method also includes identifying a target region within the area of interest of the test cartridge, evaluating a quality of the image based on a characteristic feature of the target region, and providing commands to adjust an optical coupling in the image-capturing device when the quality of the image is lower than a selected threshold.

Abstract: A method to assess a subject diagnosis is provided. The method includes receiving an image from an image-capturing device, the image comprising an area of interest in a test cartridge, and finding a border of the area of interest of the test cartridge and applying a geometrical transformation on an area delimited by the border of the test cartridge to bring the image of the area of interest in the test cartridge to a selected size and a selected shape. The method also includes identifying a target region within the area of interest of the test cartridge, evaluating a quality of the image based on a characteristic feature of the target region, and providing commands to adjust an optical coupling in the image-capturing device when the quality of the image is lower than a selected threshold.

Abstract: Provided are systems and methods for treating a biological fluid, e.g., to inactivate pathogens.

Abstract: Provided are systems and methods for treating a biological fluid, e.g., to inactivate pathogens.

Abstract: A method for performing a lateral flow assay is provided. The method includes inserting a sample cartridge (201) in a dark chamber (220) and activating a light emitter (251) in the dark chamber (220). The method includes focusing an optical coupling mechanism (115a, 115b) in an image-capturing device (100a, 100b) to optimize an image of a sensitive area (202) in the sample cartridge (201) and capturing, with an image capturing device (100a, 100b), an image of a sensitive area (202) in the sample cartridge (201) after a selected period of time. The method also includes providing the image of the sensitive area (202) to a processor, wherein the processor comprises an image-capturing application (122). A system and a computer-implemented method to perform at least partially the above method are also provided.

Abstract: A circuit in an image-capturing device is provided. The circuit includes a light source controller configured to provide a signal to a light source when a cartridge mount has received, inside a dark chamber, a test cartridge. The circuit also includes a sensor array controller configured to activate at least one pixel in a sensor array when the light source is activated, and to receive a signal from the at least one pixel, the signal indicative of an optical intensity of a light emitted from the test cartridge. The circuit also includes a processor, configured to form a transmittable file with the signal from the at least one pixel, and a radio-frequency antenna configured to transmit the transmittable file to an external processor.

Abstract: A method to assess a subject diagnosis is provided. The method includes receiving an image from an image-capturing device, the image comprising an area of interest in a test cartridge, and finding a border of the area of interest of the test cartridge and applying a geometrical transformation on an area delimited by the border of the test cartridge to bring the image of the area of interest in the test cartridge to a selected size and a selected shape. The method also includes identifying a target region within the area of interest of the test cartridge, evaluating a quality of the image based on a characteristic feature of the target region, and providing commands to adjust an optical coupling in the image-capturing device when the quality of the image is lower than a selected threshold.

Abstract: The present invention relates to the preparation and use in recipients of CAR-T cell-derived effector cells which are modified to limit their proliferation within the recipient. This is accomplished through the introduction of adducts into the nucleic acids of CAR-T cell-derived effector cells following expansion in vitro to provide expanded and activated CAR-T cell-derived effector cells that retain immunologic function, including the expression of one ore more cytokines.

Abstract: Provided are systems and methods for treating a biological fluid, e.g., to inactivate pathogens.

Abstract: The present disclosure relates to the preparation and use of CAR-NK cells which are modified by a nucleic acid targeting compound.

Abstract: Provided are methods, kits, and compositions for preparing platelet compositions suitable for infusion, including improved methods, compositions, and kits for pathogen inactivation of an apheresis-derived preparation of platelets.

Abstract: The present disclosure relates to improved methods for the preparation and platelet products for administration to subjects in need thereof

Abstract: The present invention relates to the preparation and use in recipients of CAR-T cell-derived effector cells which are modified to limit their proliferation within the recipient. This is accomplished through the introduction of adducts into the nucleic acids of CAR-T cell-derived effector cells following expansion in vitro to provide expanded and activated CAR-T cell-derived effector cells that retain immunologic function, including the expression of one ore more cytokines.

Abstract: Methods are provided for improved quenching of undesired side reactions upon treating a red blood cell composition with a pathogen inactivating compound comprising a nucleic acid binding ligand and a functional group which is, or which is capable of forming, an electrophilic group. In some embodiments, the improved methods use a suitably high concentration of quencher that comprises a nucleophilic functional group that is capable of covalently reacting with the electrophilic group, wherein the treatment occurs within a desired pH range to provide sufficient quenching. Preferred quenchers for use in some of the methods include thiols, such as glutathione, which have been suitably neutralized such that addition to a red blood cell composition results in the desired concentration of quencher at a desirable pH range of 6.8 to 8.5.

Abstract: Methods are provided for quenching undesired side reactions of pathogen inactivating compounds in biological materials comprising red blood cells. In a particular embodiment, methods are provided for quenching undesired side reactions of a pathogen inactivating compound that includes a functional group which is, or which is capable of forming, an electrophilic group. In this embodiment, the material is treated with the pathogen inactivating compound and a quencher, wherein the quencher comprises a nucleophilic functional group that is capable of covalently reacting with the electrophilic group. The electrophilic group on the pathogen inactivating compound is preferably a non-radical cationic group. In one embodiment, the pathogen inactivating compound includes a nucleic acid binding ligand and a mustard group, wherein the mustard group is capable of reacting in situ to form the electrophilic group. Preferred quenchers are thiols, such as glutathione.