Abstract: The present disclosure describes methods and systems directed towards automating tournaments within a gaming environment. Data regarding a first set of players that registered for a tournament may be received by a tournament server. The players are placed in a tournament queue. If the number of players in the tournament queue exceeds a certain threshold, then one or more tournaments are created based on a model tournament, the maximum size of the tournament, and the number of players in the tournament queue.

Abstract: CRISPR-based diagnostic methods and compositions are provided. One embodiment provides the use of DNA-barcoded antibodies or peptide-MHC (pMHC) tetramers (e.g., Kb-OVA257-264, Db-GP10025-33, Db-GP33-41) and CRISPR-Cas protein, and a guided DNA endonuclease, to achieve ultrasensitive detection of soluble and cell surface proteins. The disclosed embodiments can use type V: Cas12a; type VI: Cas13a, or Cas13b. Combining DNA encoding with CRISPR-Cas protein recognition is a sensitive system because barcodes can be isothermally amplified and Cas, for example Cas12a, enzymatically cleaves DNA reporters upon barcode detection, providing two rounds of amplification and enabling measurement of protein concentration by sample fluorescence or using by paper-based assays.

Abstract: The disclosure provides methods of treating cancer, tumors, and neoplasias by administering ionizing radiation and an anti-DLL4 antibody or a DLL4-binding fragment.

Abstract: A system for conducting wireless site surveys of a wireless environment is provided. A wireless interface controller controls a wireless interface during a scan of a wireless frequency band. A user interface module receives a set of wireless network design criteria, and a measurement module obtains a set of wireless signal information based on a set of wireless signals received during the scan of the wireless frequency band. An analysis module compares the set of wireless signal information to the set of wireless network design criteria and automatically determines whether the set of wireless network design criteria is, at least in part, satisfied. The user interface module provides an indicator that indicates whether the set of wireless network design criteria is, at least in part, satisfied.

Abstract: The disclosure provides methods of treating cancer, tumors, and neoplasias by administering ionizing radiation and an anti-DLL4 antibody or a DLL4-binding fragment.

Abstract: The present invention relates to a method for assessing a hypoxia phenotype of a tumour of a subject in which the gene expression of between 3 and 50 hypoxia-related genes of a sample obtained from said tumour of the subject is determined, thereby obtaining a sample expression profile of said hypoxia-related genes. The sample gene expression profile is then compared with a reference expression profile of said hypoxia-related genes. The hypoxia-related genes comprise at least SLC2A1, VEGFA and PGAM1. Probes, arrays and kits for use in the method are also disclosed.

Abstract: Identified herein is the location of the MN protein binding site, and MN proteins/polypeptides that compete for attachment to vertebrate cells with immobilized MN protein. Such MN proteins/polypeptides prevent cell-cell adhesion and the formation of intercellular contacts. The MN protein binding site is a therapeutic target that can be blocked by organic or inorganic molecules, preferably organic molecules, more preferably proteins/polypeptides that specifically bind to that site. Therapeutic methods for inhibiting the growth of preneoplastic/neoplastic vertebrate cells that abnormally express MN protein are disclosed. Vectors are provided that encode the variable domains of MN-specific antibodies and a flexible linker polypeptide separating those domains. Further vectors are disclosed that encode a cytotoxic protein/polypeptide operatively linked to the MN gene promoter or a MN gene promoter fragment comprising the HIF-1 consensus binding sequence, and which vectors preferably further encode a cytokine.

Abstract: The MN/CA IX protein is identified herein as a hypoxia marker. The MN/CA9 gene promoter is characterized, and the location of the HIF-1 binding site within the MN/CA9 promoter is identified. Further, the hypoxia inducibility of the MN/CA9 gene and the uses of such inducibility are disclosed. In one aspect, the invention provides diagnostic/prognostic tools for determining the presence of hypoxia in a tissue in a vertebrate, preferably a human, and for measuring the relative degree of hypoxia in said vertebrate. In another aspect, the invention provides methods using tumor biopsies to predict the radioresistance of a preneoplastic/neoplastic tissue in a vertebrate subject, preferably a human patient, for diseases in which MN/CA IX levels can be used to indicate radiobiologically relevant tumor hypoxia. Such predictive methods can be used as an aid in patient therapy selection.