Abstract: Methods and products are disclosed for adapting fragmented nucleic acids for next generation sequencing, including providing numerical codes based on variable adapter lengths for the strand and the fragment, respectively, of the fragmented nucleic acids. The methods and products allow for the amplification of the fragmented nucleic acids when there is a low yield of isolated fragmented nucleic acids and also for efficient and reliable detection of low-frequency mutations including in subpopulations of cells within a subject.

Abstract: Methods and products are disclosed for adapting fragmented nucleic acids for next generation sequencing, including providing numerical codes based on variable adapter lengths for the strand and the fragment, respectively, of the fragmented nucleic acids. The methods and products allow for the amplification of the fragmented nucleic acids when there is a low yield of isolated fragmented nucleic acids and also for efficient and reliable detection of low-frequency mutations including in subpopulations of cells within a subject.

Abstract: Methods are disclosed for adding adapters to fragmented nucleic acids for next generation sequencing, including providing numerical codes based on variable adapter molecular barcode lengths on both sides of the fragmented nucleic acids and identifying reads from the same fragment based on both barcodes. The methods and products allow for the amplification of the fragmented nucleic acids when there is a low yield of isolated fragmented nucleic acids and also for efficient and reliable detection of low-frequency mutations including in subpopulations of cells within a subject.

Abstract: A method for analyzing various regions of a genome at different resolutions, is disclosed herein, wherein the method comprises: producing, a whole-genome sequencing (WGS) library, wherein the WGS library is created from at least one of: DNA, RNA, and TNA; enriching, the WGS library, for each of one or more regions of interest; producing, a final sequencing library, wherein a first grouping of genomic regions are represented at a higher coverage than a second grouping of genomic regions; applying, NGS sequencing, to the final sequencing library, creating genetic data; and analyzing, the genetic data, to identify genetic markers.

Abstract: The present invention is directed to the probes for detecting known and unknown fusion genes, related methods of detection of fusion genes, uses and kits related thereto. In particular, the invention relates to methods of diagnosing and monitoring of a cancer.

Abstract: The present invention is directed to the probes for detecting known and unknown fusion genes, related methods of detection of fusion genes, uses and kits related thereto. In particular, the invention relates to methods of diagnosing and monitoring of a cancer.

Abstract: The present invention is directed to the probes for detecting known and unknown fusion genes, related methods of detection of fusion genes, uses and kits related thereto. In particular, the invention relates to methods of diagnosing and monitoring of a cancer.

Abstract: Methods are disclosed for adding adapters to fragmented nucleic acids for next generation sequencing, including providing numerical codes based on variable adapter molecular barcode lengths on both sides of the fragmented nucleic acids and identifying reads from the same fragment based on both barcodes. The methods and products allow for the amplification of the fragmented nucleic acids when there is a low yield of isolated fragmented nucleic acids and also for efficient and reliable detection of low-frequency mutations including in subpopulations of cells within a subject.