Patents by Inventor Agnes Ullmann
Agnes Ullmann has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20030175735Abstract: The invention concerns a recombinant adenylcyclase, comprising at least a polypeptide sequence including one or several cleavage site of at least a molecule with site-specific proteolytic activity, said polypeptide sequence being inserted in the catalytic domain of an adenylcyclase while preserving its enzymatic activity. The invention also concerns methods for screening molecules with proteolytic activity using said recombinant adenylcyclase.Type: ApplicationFiled: December 18, 2002Publication date: September 18, 2003Inventors: Gouzel Karimova, Daniel Ladant, Agnes Ullmann, Nathalie Dautin
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Publication number: 20020106783Abstract: A signal amplification system comprises a bacterial multi-hybrid system, and more preferably a two-hybrid system, of at least two chimeric polypeptides containing a first chimeric polypeptide corresponding to a first fragment of an enzyme and a second chimeric polypeptide corresponding to a second fragment of an enzyme or a modulating substance capable of activating said enzyme. The first fragment is fused to a molecule of interest and the second fragment or the modulating substance is fused to a target ligand. The activity of the enzyme is restored by the in vivo interaction between the molecule of interest and the target ligand. Signal amplification is generated and, for example, triggers transcriptional activation. The signal amplification system is useful in a method of selecting a molecule of interest, which is capable of binding to target ligand, wherein the interaction between the molecule of interest and the target ligand is detected with the signal amplification system as a kit therefor.Type: ApplicationFiled: October 10, 2001Publication date: August 8, 2002Applicant: Institut PasteurInventors: Daniel Ladant, Gouzel Karimova, Agnes Ullmann
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Publication number: 20020045237Abstract: Two bacterial strains deficient in endogenous adenylate cyclase activity are provided. The strains, designated BTH101 and DHM1, are useful in a signal amplification system comprising a bacterial multi-hybrid system, and more preferably a two-hybrid system, of at least two chimeric polypeptides containing a first chimeric polypeptide corresponding to a first fragment of an enzyme and a second chimeric polypeptide corresponding to a second fragment of an enzyme or a modulating substance capable of activating said enzyme as described in published PCT application WO 99/28746. The signal amplification system is useful in a method of selecting a molecule of interest, which is capable of binding to target ligand, wherein the interaction between the molecule of interest and the target ligand is detected with the signal amplification system, and the kit therefor, as described in WO 99/28746.Type: ApplicationFiled: March 28, 2001Publication date: April 18, 2002Inventors: Gouzel Karimova, Daniel Ladant, Agnes Ullmann, Luc Selig, Pierre Legrain
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Patent number: 6333154Abstract: A signal amplification system comprises a bacterial multi-hybrid system, and more preferably a two-hybrid system, of at least two chimeric polypeptides containing a first chimeric polypeptide corresponding to a first fragment of an enzyme and a second chimeric polypeptide corresponding to a second fragment of an enzyme or a modulating substance capable of activating said enzyme. The first fragment is fused to a molecule of interest and the second fragment or the modulating substance is fused to a target ligand. The activity of the enzyme is restored by the in vivo interaction between the molecule of interest and the target ligand. Signal amplification is generated and, for example, triggers transcriptional activation. The signal amplification system is useful in a method of selecting a molecule of interest, which is capable of binding to target ligand, wherein the interaction between the molecule of interest and the target ligand is detected with the signal amplification system as a kit therefor.Type: GrantFiled: December 1, 1998Date of Patent: December 25, 2001Assignee: Institut PasteurInventors: Daniel Ladant, Gouzel Karimova, Agnes Ullmann
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Patent number: 6017711Abstract: A test kit for the identification of various bacterial species groupings comprises a DNA probe which can identify palindromic units specific to particular bacterial species or species groupings. Such probes are highly specific for particular species or species subgroups. Accordingly, specific identification of bacteria may be achieved.Type: GrantFiled: August 21, 1998Date of Patent: January 25, 2000Assignee: Institut PasteurInventors: Eric Gilson, Jean-Marie Clement, David Perrin, Agnes Ullmann, Maurice Hofnung
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Patent number: 5935580Abstract: This invention relates to a recombinant plasmid comprising the cyaC and the cyaA genes of Bordetella which directs the expression of Bordetella adenylate cyclase in a transformed host cell. This invention also relates to a recombinat DNA molecule comprising the Bordetella cyaA gene containing at least one insertion of a heterologous DNA sequence at at least one permissive site. This invention further relates to recombinant Bordetella adenylate cyclase comprising a heterologous epitope at a permissive site as well as methods of inducing a specific B cell, helper T cell, and CTL cell immune response.Type: GrantFiled: February 27, 1995Date of Patent: August 10, 1999Assignee: Institut PasteurInventors: Daniel Ladant, Claude Leclerc, Peter Sebo, Agnes Ullmann
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Patent number: 5863721Abstract: A test kit for the identification of various bacterial species groupings comprises a DNA probe which can identify palindromic units specific to particular bacterial species or species groupings. Such probes are highly specific for particular species or species subgroups. Accordingly, specific identification of bacteria may be achieved.Type: GrantFiled: June 7, 1995Date of Patent: January 26, 1999Assignee: Institut PasteurInventors: Eric Gilson, Jean-Marie Clement, David Perrin, Agnes Ullmann, Maurice Hofnung
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Patent number: 5837455Abstract: Fragments of nucleic acids derived from an appropriate mycobacteria genome, particularly Mycobacterium tuberculosis, their applications in the diagnosis of mycobacteria infections, as well as plasmides containing said fragments. The nucleotidic sequence is comprised of a nucleotidic sequence repeated in the genome of a mycobacterium and specific of the bacillus of tuberculosis and is characterized by a strong hybridation with M. tuberculosis.Type: GrantFiled: June 7, 1995Date of Patent: November 17, 1998Assignee: Institut PasteurInventors: Jean-Luc Guesdon, Dominique Thierry, Agnes Ullmann, Brigitte Gicquel, Anne Brisson-Noel
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Patent number: 5679784Abstract: A recombinant plasmid comprises the cyaC and the cyaA genes of Bordetella which directs the expression of Bordetella, adenylate cyclase in a transformed host cell. A recombinant DNA molecule can comprise the Bordetella cyaA gene containing at least one insertion of a heterologous DNA sequence at at least one permissive site. In addition, a recombinant Bordetella adenylate cyclase comprises a heterologous epitope at a permissive site. Methods of inducing a specific B cell, helper T cell, and CTL cell immune response are provided.Type: GrantFiled: June 7, 1995Date of Patent: October 21, 1997Assignee: Institut PasteurInventors: Daniel Ladant, Claude Leclerc, Peter Sebo, Agnes Ullmann
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Patent number: 5503829Abstract: A recombinant plasmid comprises the cyaC and the cyaA genes of Bordetella which directs the expression of Bordetella, adenylate cyclase in a transformed host cell. A recombinant DNA molecule can comprise the Bordetella cyaA gene containing at least one insertion of a heterologous DNA sequence at at least one permissive site. In addition, a recombinant Bordetella adenylate cyclase comprises a heterologous epitope at a permissive site. Methods of inducing a specific B cell, helper T cell, and CTL cell immune response are provided.Type: GrantFiled: November 7, 1994Date of Patent: April 2, 1996Assignee: Institut PasteurInventors: Daniel Ladant, Claude Leclerc, Peter Sebo, Agnes Ullmann
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Patent number: 5492811Abstract: A test kit for the identification of various bacterial species groupings comprises a DNA probe which can identify palindromic units specific to particular bacterial species or species groupings. Such probes are highly specific for particular species or species subgroups. Accordingly, specific identification of bacteria may be achieved.Type: GrantFiled: December 10, 1993Date of Patent: February 20, 1996Assignee: Institut PasteurInventors: Eric Gilson, Jean-Marie Clement, David Perrin, Agnes Ullmann, Maurice Hofnung
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Patent number: 5183745Abstract: The adenyl cyclase derivatives are atoxic derivatives in which one or several amino acids are different from those present in the toxic protein, the antibodies formed against these derivatives recognize, however, the toxic protein. These derivatives can be used as vaccines.Type: GrantFiled: October 25, 1989Date of Patent: February 2, 1993Assignee: Institut PasteurInventors: Antoine Danchin, Philippe Glaser, Evelyne Krin, Octavien Barzu, Daniel Ladant, Agnes Ullmann