Abstract: The invention relates to a method for decellularization of tissue samples to produce decellularized extracellular matrix (dECM) by performing one or more wash cycles in a reactor using a reagent after at least partially submerging the tissue sample in the reagent, wherein the reactor comprising a tissue chamber in fluid communication with a reactor chamber. The method further comprising the steps of agitating the tissue sample and/or the reagent such that the tissue sample circulates in the reagent while retaining the tissue sample in a tissue chamber or a decellularization reactor, monitoring at least one parameter of the reagent and/or at least one parameter of the tissue sample, and adjusting one or more wash cycles based on said monitoring. The invention further relates to a decellularization reactor and a decellularization system for decellularizing a tissue sample.

Abstract: Disclosed is a device for cell culture, the device includes a first chamber having an area for containing cells, such as isolated cells, for growth; a second chamber for containing culture medium; a semi permeable membrane forming at least part of a divider between the first chamber and the second chamber; wherein the area for containing cells for growth is adjustable via a moveable wall disposed within the first chamber. The device is especially suited for culturing of semi-adherent and non-adherent cells.