Abstract: Methods and systems for detecting particles. In an exemplary method, a sample fluid including the particles may be driven from a sample inlet channel, through a confluence region, and into a sample outlet channel defining a longitudinal axis. Focusing fluid may be introduced into the confluence region from at least two focusing channels along respective introduction axes. Introducing may be rotationally asymmetrical about the longitudinal axis. The introduction axes and the longitudinal axis may collectively extend in three dimensions. The particles may be passed through an interrogation zone of the sample outlet channel. The interrogation zone may be irradiated with light. Optical radiation may be detected from the interrogation zone.

Abstract: Valve assemblies and related systems are disclosed. An apparatus includes or comprises a system including or comprising an imaging system, a flow cell interface having a corresponding flow cell receptacle, a stage assembly, and a valve assembly including or comprising a valve and a valve drive assembly. The stage assembly moves the flow cell interface relative to the imaging system and the valve drive assembly is to drive the valve using shaped input signals to reduce vibration imposed on at least one of the stage assembly or another component of the apparatus based on movement of the valve.