Patents by Inventor Ahmed Chenna
Ahmed Chenna has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10538549Abstract: NNA-derived specific adducts represent an integrated biomarker of exposure to thirdhand smoke (THS) as NNA is unique to THS. The NNA-dG covalent binding adduct could serve as such a biomarker, and play a role in identifying individuals exposed to THS, thus providing critical information for early detection and prevention.Type: GrantFiled: March 16, 2015Date of Patent: January 21, 2020Assignee: The Regents of the University of CaliforniaInventors: Bo Hang, Ahmed Chenna
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Patent number: 8357277Abstract: The invention relates to an enhanced method of detecting and/or quantifying at least one analyte in a sample.Type: GrantFiled: November 26, 2008Date of Patent: January 22, 2013Assignee: Laboratory Corp. of America HoldingsInventors: Youssouf Badal, Ahmed Chenna, Syed Hasan Tahir, Yuping Tan
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Publication number: 20110180408Abstract: The invention relates to an enhanced method of detecting and/or quantifying at least one analyte in a sample.Type: ApplicationFiled: November 26, 2008Publication date: July 28, 2011Inventors: Youssouf Badal, Ahmed Chenna, Syed Hasan Tahir, Yuping Tang
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Publication number: 20060223107Abstract: The present invention is directed to a method of detecting pluralities of target nucleic acid sequences by forming and cleaving duplex structures with a pair of probes, one probe of each pair being labeled with an electrophoretic tag. Cleavage of the duplex structures releases electrophoretic tags that are then separated and identified to indicate the presence or quantity of the target sequences. The present invention is particularly useful in multiplex reactions wherein multiple target sequences are detected in one reaction. Kits useful in the detection of nucleic acids are also provided.Type: ApplicationFiled: June 5, 2006Publication date: October 5, 2006Inventors: Ahmed Chenna, Vivian Xiao, Sharat Singh
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Patent number: 7037654Abstract: Probe sets for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. Detection involves the release of identifying tags as a consequence of target recognition. The probe sets include electrophoretic tag probes or e-tag probes, comprising a detection region and a mobility-defining region called the mobility modifier, both linked to a target-binding moiety. The probes comprise interactive functionalities adjacent the cleaved portion positioned in the probes such that the interactive functionality does not form part of the e-tag reporters. Target antiligands are contacted with a set of e-tag probes and the contacted antiligands are treated with a selected cleaving agent resulting in a mixture of e-tag reporters and uncleaved and/or partially cleaved e-tag probes. The mixture is exposed to a capture agent effective to bind to uncleaved or partially cleaved e-tag probes, followed by electrophoretic separation.Type: GrantFiled: November 9, 2001Date of Patent: May 2, 2006Assignee: Aclara Biosciences, Inc.Inventors: Ahmed Chenna, Sharat Singh
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Patent number: 7001725Abstract: Kits for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. Detection involves the release of identifying tags as a consequence of target recognition. The kits include sets of electrophoretic tag probes or e-tag probes, a capture agent and optionally a cleaving agent. The e-tag probes comprise a detection region and a mobility-defining region called the mobility modifier, both linked to a target-binding moiety. In using the kits, target antiligands are contacted with a set of e-tag probes and the contacted antiligands are treated with a selected cleaving agent resulting in a mixture of e-tag reporters and uncleaved and/or partially cleaved e-tag probes. The mixture is exposed to a capture agent effective to bind to uncleaved or partially cleaved e-tag probes, followed by electrophoretic separation. In a multiplexed assay, different released e-tag reporters may be separated and detected providing for target identification.Type: GrantFiled: April 2, 2001Date of Patent: February 21, 2006Assignee: Aclara Biosciences, Inc.Inventors: Sharat Singh, Tracy Matray, Ahmed Chenna
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Patent number: 6955874Abstract: Kits for the multiplexed detection of known, selected nucleotide target sequences are provided. Detection involves the release of identifying tags as a consequence of target recognition. The kits include sets of electrophoretic tag probes or e-tag probes, capture agent and optionally a nuclease. The e-tag probes comprise a detection region and a mobility-defining region called the mobility modifier, both linked to a target-binding moiety. In using the kits, the target-binding moiety of the e-tag probes hybridizes to complementary target sequences followed by nuclease cleavage of the e-tag probes and release of detectable e-tags or e-tag reporters. The mixture is exposed to a capture agent which binds uncleaved and/or partially cleaved e-tag probes, followed by electrophoretic separation. In a multiplexed assay, different released e-tag reporters may be separated and detected providing for target identification.Type: GrantFiled: April 2, 2001Date of Patent: October 18, 2005Assignee: Aclara Biosciences, Inc.Inventors: Sharat Singh, Tracy Matray, Ahmed Chenna
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Patent number: 6916612Abstract: Probe sets for the multiplexed detection of known, selected nucleotide target sequences are provided. Detection involves the release of identifying tags as a consequence of target recognition. The probe sets include electrophoretic tag probes or “e-tag probes”, comprising a detection region and a mobility-defining region called the mobility modifier, both linked to a target-binding moiety. The target-binding moiety of the e-tag probes hybridizes to complementary target sequences followed by nuclease cleavage of the e-tag probes and release of detectable e-tags or e-tag reporters. The mixture is exposed to a capture agent which binds uncleaved and/or partially cleaved e-tag probes, followed by electrophoretic separation. In a multiplexed assay, different released e-tag reporters may be separated and detected providing for target identification.Type: GrantFiled: April 2, 2001Date of Patent: July 12, 2005Assignee: Aclara Biosciences, Inc.Inventors: Sharat Singh, Tracy Matray, Ahmed Chenna
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Publication number: 20050053939Abstract: Probe sets for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. Detection involves the release of identifying tags as a consequence of target recognition. The probe sets include electrophoretic tag probes or e-tag probes, comprising a detection region and a mobility-defining region, both linked to a target-binding moiety. In a multiplexed assay, different released e-tag reporters may be separated and detected providing for target identification. The probes comprise interactive functionalities adjacent the cleaved portion positioned in the probes such that the interactive functionality does not form part of the e-tag reporters. Also described are biopolymers and nucleosides containing such interactive functionalities.Type: ApplicationFiled: November 8, 2002Publication date: March 10, 2005Inventors: Ahmed Chenna, Sharat Singh
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Publication number: 20050048553Abstract: Methods and kits are disclosed for determining, either in a homogeneous or heterogeneous assay format, one or more target analytes in a sample using binding compositions coupled to molecular tags by cleavable linkages. Generally, an assay mixture is formed comprising a sample and a reagent comprising multiple such binding compositions under conditions that permit stable complexes to form between the binding compositions and analytes. In one aspect of the invention, the interaction between the binding compositions and their respective binding sites brings a cleavage-inducing moiety into close proximity to cleavable linkages or provides a recognizable substrate for a cleavage-inducing moiety. In this way, one or more molecular tags for each of the analytes are released from the complexes. Released molecular tags are chromatographically separated and the presence and/or amount of the target analytes are determined based on the analysis of the released and separated molecular tags.Type: ApplicationFiled: August 16, 2004Publication date: March 3, 2005Inventors: Ahmed Chenna, Tracy Matray, Vincent Hernandez, Herbert Hooper, Sharat Singh
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Publication number: 20040265858Abstract: Probe sets for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. Detection involves the release of identifying tags as a consequence of target recognition. The probe sets include electrophoretic tag probes or e-tag probes, comprising a detection region and a mobility-defining region called the mobility modifier, both linked to a target-binding moiety. Target antiligands are contacted with a set of e-tag probes and the contacted antiligands are treated with a selected cleaving agent resulting in a mixture of e-tag reporters and uncleaved and/or partially cleaved e-tag probes. The mixture is exposed to a capture agent effective to bind to uncleaved or partially cleaved e-tag probes, followed by electrophoretic separation. In a multiplexed assay, different released e-tag reporters may be separated and detected providing for target identification.Type: ApplicationFiled: February 13, 2004Publication date: December 30, 2004Inventors: Sharat Singh, Tracy Matray, Ahmed Chenna
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Publication number: 20040166529Abstract: Probe sets for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. Detection involves the release of identifying tags as a consequence of target recognition. The probe sets include electrophoretic tag probes or e-tag probes, comprising a detection region and a mobility-defining region called the mobility modifier, both linked to a target-binding moiety. Target antiligands are contacted with a set of e-tag probes and the contacted antiligands are treated with a selected cleaving agent resulting in a mixture of e-tag reporters and uncleaved and/or partially cleaved e-tag probes. The mixture is exposed to a capture agent effective to bind to uncleaved or partially cleaved e-tag probes, followed by electrophoretic separation. In a multiplexed assay, different released e-tag reporters may be separated and detected providing for target identification.Type: ApplicationFiled: April 21, 2004Publication date: August 26, 2004Inventors: Sharat Singh, Tracy Matray, Ahmed Chenna
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Patent number: 6770439Abstract: Probe sets for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. Detection involves the release of identifying tags as a consequence of target recognition. The probe sets include electrophoretic tag probes or e-tag probes, comprising a detection region and a mobility-defining region called the mobility modifier, both linked to a target-binding moiety. Target antiligands are contacted with a set of e-tag probes and the contacted antiligands are treated with a selected cleaving agent resulting in a mixture of e-tag reporters and uncleaved and/or partially cleaved e-tag probes. The mixture is exposed to a capture agent effective to bind to uncleaved or partially cleaved e-tag probes, followed by electrophoretic separation. In a multiplexed assay, different released e-tag reporters may be separated and detected providing for target identification.Type: GrantFiled: April 2, 2001Date of Patent: August 3, 2004Inventors: Sharat Singh, Tracy Matray, Ahmed Chenna
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Publication number: 20040096825Abstract: Probe sets for the multiplexed detection of the binding of, or interaction between, one or more ligands and target antiligands are provided. Detection involves the release of identifying tags as a consequence of target recognition. The probe sets include electrophoretic tag probes or e-tag probes, comprising a detection region and a mobility-defining region called the mobility modifier, both linked to a target-binding moiety. The probes comprise interactive functionalities adjacent the cleaved portion positioned in the probes such that the interactive functionality does not form part of the e-tag reporters. Target antiligands are contacted with a set of e-tag probes and the contacted antiligands are treated with a selected cleaving agent resulting in a mixture of e-tag reporters and uncleaved and/or partially cleaved e-tag probes. The mixture is exposed to a capture agent effective to bind to uncleaved or partially cleaved e-tag probes, followed by electrophoretic separation.Type: ApplicationFiled: November 9, 2001Publication date: May 20, 2004Applicant: Aclara BioSciences, Inc.Inventors: Ahmed Chenna, Sharat Singh
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Publication number: 20040067498Abstract: The present invention is directed to a method of detecting pluralities of target nucleic acid sequences by forming and cleaving duplex structures with a pair of probes, one probe of each pair being labeled with an electrophoretic tag. Cleavage of the duplex structures releases electrophoretic tags that are then separated and identified to indicate the presence or quantity of the target sequences. The present invention is particularly useful in multiplex reactions wherein multiple target sequences are detected in one reaction. Kits useful in the detection of nucleic acids are also provided.Type: ApplicationFiled: November 6, 2002Publication date: April 8, 2004Inventors: Ahmed Chenna, Vivian Xiao, Sharat Singh
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Publication number: 20030235832Abstract: Methods and kits are disclosed for determining, either in a homogeneous or heterogeneous assay format, one or more target analytes in a sample using binding compositions coupled to molecular tags by cleavable linkages. Generally, an assay mixture is formed comprising a sample and a reagent comprising multiple such binding compositions under conditions that permit stable complexes to form between the binding compositions and analytes. In one aspect of the invention, the interaction between the binding compositions and their respective binding sites brings a cleavage-inducing moiety into close proximity to cleavable linkages or provides a recognizable substrate for a cleavage-inducing moiety. In this way, one or more molecular tags for each of the analytes are released from the complexes. Released molecular tags are chromatographically separated and the presence and/or amount of the target analytes are determined based on the analysis of the released and separated molecular tags.Type: ApplicationFiled: November 8, 2002Publication date: December 25, 2003Inventors: Ahmed Chenna, Tracy Matray, Vincent Hernandez, Herbert Hooper, Sharat Singh
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Patent number: 6649351Abstract: The invention provides a method for detecting a target analyte, by: (a) contacting one or more target analytes with a set of first and second binding reagents under conditions sufficient for binding of a target analyte with the first and second binding reagents, each of the first binding reagents containing a cleavage-inducing moiety and a target binding moiety, each of the second binding reagents containing a tagged probe having a mass modifier region attached to a target binding moiety by a cleavable linkage, the cleavable linkage being susceptible to cleavage when in proximity to an activated cleavage-inducing moiety; (b) activating the cleavage-inducing moiety to release a tag reporter, and (c) detecting a mass of the tag reporter, the mass uniquely corresponding to a known target analyte.Type: GrantFiled: November 9, 2001Date of Patent: November 18, 2003Assignee: Aclara Biosciences, Inc.Inventors: Tracy J. Matray, Vincent S. Hernandez, Ahmed Chenna, Herbert Hooper, Sharat Singh
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Publication number: 20030211565Abstract: Cytochrome P-450 assay methods and kits for the methods are provided employing a cytochrome P-450 enzyme, substrates characterized by having an oxidizable methylene group oxidized to an aldehyde and a fluorescent hydrazine. A fluorescent hydrazine is added to the reaction mixture and the resulting hydrazone analyzed by capillary electrophoresis. The method finds use in evaluating compounds for enzyme modulating activity.Type: ApplicationFiled: June 19, 2003Publication date: November 13, 2003Inventors: Sharat Singh, Ahmed Chenna, Hossein Salimi-Moosavi, Ian Gibbons
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Patent number: 6632630Abstract: Cytochrome P-450 assay methods and kits for the methods are provided employing a cytochrome P-450 enzyme, substrates characterized by having an oxidizable methylene group oxidized to an aldehyde and a fluorescent hydrazine. A fluorescent hydrazine is added to the reaction mixture and the resulting hydrazone analyzed by capillary electrophoresis. The method finds use in evaluating compounds for enzyme modulating activity.Type: GrantFiled: March 28, 2001Date of Patent: October 14, 2003Assignee: Aclara Biosciences, Inc.Inventors: Sharat Singh, Ahmed Chenna, Hossein Salimi-Moosari, Ian Gibbons
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Publication number: 20030092012Abstract: The invention provides a method for detecting a target nucleic acid sequence. The method involves contacting one or more target nucleic acid sequences with a set of tagged probes under conditions sufficient for hybridization of a target nucleic acid sequence with a tagged probe, the tagged probes comprising a mobility modifier attached to a nucleic acid target binding moiety by a bond that is cleavable by a nuclease, the nucleic acid target binding moiety containing at least one bond resistant to said nuclease; treating the tagged probe hybridized to the target nucleic acid with a nuclease under conditions sufficient for cleavage of the nuclease-cleavable bond to release a tag reporter; separating a tag reporter using a chromatographic method, and detecting a tag reporter corresponding to a known target sequence.Type: ApplicationFiled: November 9, 2001Publication date: May 15, 2003Inventors: Ahmed Chenna, Tracy J. Matray, Vincent S. Hernandez, Herbert Hooper, Sharat Singh