Abstract: The invention, in part, includes compositions comprising quadruplet decoding tRNAs and their encoding sequences. The invention also includes assay methods to assess quadruplet decoding as well as methods of preparing quadruplet decoding suppression tRNAs.

Abstract: One or more example relate, generally, to generating radio frequency (RF) signals. An apparatus may include a signal generator, an amplification stage, and a feedback control loop. The signal generator may generate a pulsed radio frequency (RF) signal at least partially responsive to a digital pulsed waveform defined by one or more commands. The amplification stage may amplify the pulsed RF signal. The feedback control loop may be coupled to the amplification stage to regulate a power level of respective steps of the pulsed RF signal.

Abstract: A method includes receiving condition data including one or more of temperature measurements, pressure measurements, humidity measurements, location coordinates, or a weather forecast for a future time period. The method includes applying, to a predictive machine learning model, the condition data and predictive data generated from a mathematical model of the physical operation of the environment system. The predictive machine learning model is configured to output optimal commands to operational components of the environmental system. The method includes applying the optimal commands to a failure machine learning model. The failure machine learning module is trained to output modified commands that address potential failures in the operational components of the environmental system. The method includes transmitting the modified commands to the operational components of the environmental system.

Abstract: A computer-implemented method, system and computer program product for recommending a template or a dataset. A descriptor (input descriptor) of the input, corresponding to a dataset or a dashboard template, is extracted or fetched. The input descriptor may be the descriptor of the dataset which includes topics and concept combinations. Alternatively, the input descriptor may be the descriptor of the dashboard template which includes topics, concept combinations and column-to-visualization mappings. Scores are then generated for the candidate targets, corresponding to dashboard templates or datasets, based on one or more of the following: matching topics in the input descriptor with topics in the candidate target descriptors, matching concept combinations in the input descriptor with concept combinations in the candidate target descriptors, and determining compatibility of the datasets with the dashboard templates.

Abstract: Engineered transcriptional activator-like effectors (TALEs) are versatile tools for genome manipulation with applications in research and clinical contexts. One current drawback of TALEs is that the 5? nucleotide of the target is specific for thymine (T). TALE domains with alternative 5? nucleotide specificities could expand the scope of DNA target sequences that can be bound by TALEs. Another drawback of TALEs is their tendency to bind and cleave off-target sequence, which hampers their clinical application and renders applications requiring high-fidelity binding unfeasible. This disclosure provides methods and strategies for the continuous evolution of proteins comprising DNA-binding domains, e.g., TALE domains. In some aspects, this disclosure provides methods and strategies for evolving such proteins under positive selection for a desired DNA-binding activity and/or under negative selection against one or more undesired (e.g., off-target) DNA-binding activities.

Abstract: An implant system for the fusion of the occipitocervical (O-C1) joint is provided. The system has a plate including a horizontal panel, a right vertical panel and a left vertical panel. The horizontal panel has at least two openings. The right vertical panel has at least two openings. The left vertical panel has at least two openings. The system also includes at least four bone fixation fasteners to fix the panels to the occipital condyle and C1 lateral mass using the openings in the panels.

Abstract: Some aspects of this disclosure provide methods for phage-assisted continuous evolution (PACE) of proteases. Some aspects of this invention provide methods for evaluating and selecting protease inhibitors based on the likelihood of the emergence of resistant proteases as determined by the protease PACE methods provided herein. Some aspects of this disclosure provide strategies, methods, and reagents for protease PACE, including fusion proteins for translating a desired protease activity into a selective advantage for phage particles encoding a protease exhibiting such an activity and improved mutagenesis-promoting expression constructs. Evolved proteases that recognize target cleavage sites which differ from their canonical cleavage site are also provided herein.

Abstract: Radio frequency (RF) generators are disclosed. A RF generator may include a modulator configured to receive an arbitrary waveform and an RF carrier, and generate a pulsed radio frequency (RF). The arbitrary waveform may be generated via an analog signal generator external to the RF generator. Further, the RF generator may include an amplification stage configured to amplify the pulsed RF signal. RF generation systems and methods of generating a pulsed RF signal also disclosed.

Abstract: Engineered transcriptional activator-like effectors (TALEs) are versatile tools for genome manipulation with applications in research and clinical contexts. One current drawback of TALEs is that the 5? nucleotide of the target is specific for thymine (T). TALE domains with alternative 5? nucleotide specificities could expand the scope of DNA target sequences that can be bound by TALEs. Another drawback of TALEs is their tendency to bind and cleave off-target sequence, which hampers their clinical application and renders applications requiring high-fidelity binding unfeasible. This disclosure provides methods and strategies for the continuous evolution of proteins comprising DNA-binding domains, e.g., TALE domains. In some aspects, this disclosure provides methods and strategies for evolving such proteins under positive selection for a desired DNA-binding activity and/or under negative selection against one or more undesired (e.g., off-target) DNA-binding activities.

Abstract: Systems and methods for heteroaromatic silicon fluoride compounds as radiopharmaceuticals with theranostic properties are described. The theranostic heteroaromatic silicon fluoride compounds can be conjugated with various disease binding ligands and/or chelators for imaging and therapeutic applications.

Abstract: Some aspects of this disclosure provide methods for phage-assisted continuous evolution (PACE) of proteases. Some aspects of this invention provide methods for evaluating and selecting protease inhibitors based on the likelihood of the emergence of resistant proteases as determined by the protease PACE methods provided herein. Some aspects of this disclosure provide strategies, methods, and reagents for protease PACE, including fusion proteins for translating a desired protease activity into a selective advantage for phage particles encoding a protease exhibiting such an activity and improved mutagenesis-promoting expression constructs. Evolved proteases that recognize target cleavage sites which differ from their canonical cleavage site are also provided herein.

Abstract: The disclosure provides amino acid sequence variants of Bacillus thuringiensis (Bt) toxins and methods of producing the same. Some aspects of this disclosure provide methods for generating Bt toxin variants by continuous directed evolution. Some aspects of this disclosure provide compositions and methods for pest control using the disclosed variant Bt toxins.

Abstract: One or more example relate, generally, to generating radio frequency (RF) signals. An apparatus may include a signal generator, an amplification stage, and a feedback control loop. The signal generator may generate a pulsed radio frequency (RF) signal at least partially responsive to a digital pulsed waveform defined by one or more commands. The amplification stage may amplify the pulsed RF signal. The feedback control loop may be coupled to the amplification stage to regulate a power level of respective steps of the pulsed RF signal.

Abstract: A method, system, and computer program product for implementing machine learning visual code and action generation is provided. The method includes receiving from a plurality of hardware and software sources, digital description data associated with visual presentations and an action for execution. A resulting code-based class for each portion of the digital description data is generated with respect to the visual presentation. Self learning software code is executed and a type of visual presentation is selected with respect to associated visual features and the code-based class. Additionally, a visual presentation is selected and an action is executed resulting in hardware and software of a server hardware device being operationally modified. The visual presentation is presented to a user.

Abstract: Radio frequency (RF) generators are disclosed. A RF generator may include a modulator configured to receive an arbitrary waveform and an RF carrier, and generate a pulsed radio frequency (RF). The arbitrary waveform may be generated via an analog signal generator external to the RF generator. Further, the RF generator may include an amplification stage configured to amplify the pulsed RF signal. RF generation systems and methods of generating a pulsed RF signal also disclosed.

Abstract: Some aspects of this disclosure relate to systems, apparatuses, compositions (e.g., isolated nucleic acids and vectors), and methods for improving the stability and/or solubility of proteins evolved using phage-assisted continuous evolution (PACE). In some embodiments, vectors described herein comprise nucleic acids encoding selection systems (e.g., positive and/or negative selection systems) that link expression of genes required for production of infectious phage particles to a desirable physiochemical (e.g., stability or solubility) and/or desired function of an evolved protein.

Abstract: A heat exchanger (4) has fluid flow channels (6) with at least one heat exchanging surface (10) which has an undulating surface section for which the surface profile varies along a predetermined direction such that at a first edge (E1) the surface profile follows a first transverse wave (20), at a second edge (E)2 the surface profile follows a second transverse wave (22) and at an intermediate point I between the edges the surface profile follows a third transverse wave (24). The third transverse wave (24) has a different phase, frequency or amplitude to the first and second transverse waves so that chevron-shaped ridges and valleys are formed. This improves the mixing of fluid passing through the channel and hence the heat exchange efficiency.

Abstract: Radio frequency (RF) generators are disclosed. A RF generator may include an analog signal generator configured to generate a pulsed analog signal responsive to a digital pulsed waveform defined by one or more commands. The RF generator may also include a modulator configured to generate a pulsed radio frequency (RF) signal by modulating an RF carrier using the pulsed analog signal as a modulating signal. Further, the RF generator may include an amplification stage configured to amplify the pulsed RF signal output by the modulator. RF generation systems and methods of generating a pulsed RF signal also disclosed.

Abstract: The disclosure provides amino acid sequence variants of Bacillus thuringiensis (Bt) toxins and methods of producing the same. Some aspects of this disclosure provide methods for generating Bt toxin variants by continuous directed evolution. Some aspects of this disclosure provide compositions and methods for pest control using the disclosed variant Bt toxins.

Abstract: A method, system, and computer program product for implementing machine learning visual code and action generation is provided. The method includes receiving from a plurality of hardware and software sources, digital description data associated with visual presentations and an action for execution. A resulting code-based class for each portion of the digital description data is generated with respect to the visual presentation. Self learning software code is executed and a type of visual presentation is selected with respect to associated visual features and the code-based class. Additionally, a visual presentation is selected and an action is executed resulting in hardware and software of a server hardware device being operationally modified. The visual presentation is presented to a user.