Abstract: The present invention relates to a non-destructive process for the preparation of amoebocyte lysate from the haemolymph of horseshoe crab, characterized by the steps of firstly, means for acclimatizing a live specimen of horseshoe crab in treated seawater under controlled conditions; then, means for cleaning the live specimen, including cleaning the base of the last pair of thoracic appendages of the live specimen; next, means for withdrawing the haemolymph through the base of the last pair of thoracic appendage under aseptic condition; then, means for transferring the collected haemolymph into a pre-cooled disposable centrifuge tube; then, means for centrifuging the collected haemolymph to separate amoebocyte cells from lymph; then, means for decanting the lymph out of the disposable centrifuge tube; then, means for washing the white amoebocyte cells and centrifuging; then, means for sonicating the amoebocyte cells for lysing of the amoebocyte cells; then, means for storing the sonicated amoebocyte cells for