Abstract: Provided is a method of detecting analytes in a cell or tissue sample, the method comprising: a) introducing into the cell or tissue sample at least one tagging moiety, wherein the tagging moieties can interact with specific proteins of interest; b) detecting analytes and tagging moieties in the cell or tissue sample; c) spatially detecting proteins in the cell or tissue sample; and d) constructing a map of the analytes in the cell or tissue sample based on the data from steps b) and c). Also provided is a microfluidic chip using the method of detecting analytes, and methods of monitoring an in situ model of a tumor, methods of detecting cancer, and methods of determining response of a subject to a treatment protocol using the microfluidic chip.

Abstract: Disclosed herein are methods of detecting multiple protein interactions within a cell or tissue sample. This can be done by multiplexed imaging. Also disclosed are systems and kits for carrying this out.

Abstract: A system of barcoding isotopically encoded particles in combination with elemental analyses and imaging that includes a particulate matrix, at least one isotope label contained in the particulate matrix, where the isotope label operates as i) an elemental identifier, ii) a mass identifier, or iii) an elemental identifier and a mass identifier, where the matrix operates as multi-digit particulate barcodes, at least i) a mass-based imager, ii) an elemental analyzer, iii) or the mass-based imager and the elemental analyzer, and a debarcoding algorithm and an automated machine learning analysis algorithm programmed on a computer to computational extract the multi-digit particulate barcodes for quantification of spatial nanotag distributions in ion beam imaging areas.

Abstract: Disclosed herein are methods and systems for analyzing visual data from multiple rounds of hybridization interactions where the same molecular target is detected by probes with different detectable labels. In particular, disclosed herein are methods and systems for analyzing sequential hybridization images for molecular profiling, where the images are obtained using multiplex fluorescence in situ hybridization (FISH).

Abstract: An imaging device uses a fiber optic faceplate (FOF) with a compressive sampling algorithm for the fluorescent imaging of a sample over an large field-of-view without the need for any lenses or mechanical scanning. The imaging device includes a sample holder configured to hold a sample and a prism or hemispherical glass surface disposed adjacent the sample holder on a side opposite the lower surface of the sample holder. A light source is configured to illuminate the sample via the prism or the hemispherical surface, wherein substantially all of the light is subject to total internal reflection at the lower surface of the sample holder. The FOF is disposed adjacent to the lower surface of the sample holder, the fiber optic array having an input side and an output side. The device includes an imaging sensor array disposed adjacent to the output side of the fiber optic array.

Abstract: Disclosed herein are methods and systems for analyzing visual data from multiple rounds of hybridization interactions where the same molecular target is detected by probes with different detectable labels. In particular, disclosed herein are methods and systems for analyzing sequential hybridization images for molecular profiling, where the images are obtained using multiplex fluorescence in situ hybridization (FISH).

Abstract: An allergy testing system for use with a mobile electronic device having a camera includes a housing that can be attached to the mobile electronic device. First and second light sources within the housing are configured to illuminate, respectively, a test sample and a control sample. A colorimetric assay is performed on the test sample and the control sample. The first light source and the second light source are activated and the camera of the mobile electronic device captures images of transmitted light. The relative intensity of transmitted light is then used by software loaded on the mobile electronic device to determine a relative absorbance value. The relative absorbance value is used, together with a calibration curve, to measure the concentration of a particular allergen within the test sample. Based on the concentration of the allergen the test sample can be labeled as either “positive” or “negative.

Abstract: An imaging device uses a fiber optic faceplate (FOF) with a compressive sampling algorithm for the fluorescent imaging of a sample over an large field-of-view without the need for any lenses or mechanical scanning. The imaging device includes a sample holder configured to hold a sample and a prism or hemispherical glass surface disposed adjacent the sample holder on a side opposite the lower surface of the sample holder. A light source is configured to illuminate the sample via the prism or the hemispherical surface, wherein substantially all of the light is subject to total internal reflection at the lower surface of the sample holder. The FOF is disposed adjacent to the lower surface of the sample holder, the fiber optic array having an input side and an output side. The device includes an imaging sensor array disposed adjacent to the output side of the fiber optic array.