Patents by Inventor Ai Hara
Ai Hara has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20210238659Abstract: An intraoral examination method for determining the state of periodontal disease is provided. The method is an intraoral examination method for measuring a signal intensity of a nucleic acid from an oral bacterial group present in an oral sample, calculating an abundance of the bacterial group from a measured value of the signal intensity, and determining the state of periodontal disease using the obtained calculated value as an index, wherein the abundance of the bacterial group shows a correlation between a bacterial load of a bacterial species that increases as a periodontal pocket value increases and a bacterial load of a bacterial species that decreases as a periodontal pocket value increases.Type: ApplicationFiled: November 2, 2018Publication date: August 5, 2021Applicant: GC CORPORATIONInventors: Naoyuki TOGAWA, Ai HARA, Shinya MURAKAMI, Takenori NOZAKI
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Publication number: 20210164028Abstract: A method for simply predicting the degree of inflammation of periodontal tissue such as an inflamed area (PISA or CAPRS value), and a device (e.g., a DNA chip) used for the method are provided. The method is a method for estimating a periodontal pocket inflammation area by detecting bacterial loads of two or more types of bacteria in saliva and using the obtained detection results as indexes, wherein bacteria to be detected include: a bacterium having a positive correlation between the bacterial load of the bacterium and the periodontal pocket inflammation area and a bacterium having a negative correlation between the bacterial load of the bacterium and the periodontal pocket inflammation area.Type: ApplicationFiled: November 2, 2018Publication date: June 3, 2021Applicant: GC CORPORATIONInventors: Naoyuki TOGAWA, Ai HARA, Shinya MURAKAMI, Takenori NOZAKI
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Publication number: 20210095335Abstract: A method for determining absolute amounts of two or more types of target nucleic acids in a sample, including: (a) mixing a sample with a known amount of a control nucleic acid; (b) co-amplifying all target nucleic acids and a specific nucleic acid group in the sample and the control nucleic acid; (c) determining a total amount of the all target nucleic acids and the specific nucleic acid group in the sample based on an indicator of a total amount of the amplified all target nucleic acids and the amplified specific nucleic acid group and an indicator of an amount of the amplified control nucleic acid; and (d) calculating an absolute amount of each target nucleic acid in the sample based on an occupancy of each target nucleic acid in the total of the all target nucleic acids and the specific nucleic acid group.Type: ApplicationFiled: November 20, 2020Publication date: April 1, 2021Applicant: Mitsubishi Chemical CorporationInventors: Kazuki Momose, Ai Hara, Naoyuki Togawa, Eiji Satou
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Patent number: 10487320Abstract: Provided is an improved nitrile hydratase with improved catalytic activity. Also provided are DNA for coding the improved nitrile hydratase, a recombinant vector that contains the DNA, a transformant that contains the recombinant vector, nitrile hydratase acquired from a culture of the transformant, and a method for producing the nitrile hydratase. Also provided is a method for producing an amide compound that uses the culture or a processed product of the culture. The improved nitrile hydratase contains an amino acid sequence represented by SEQ ID NO: 50 (GX1X2X3X4DX5X6R) in a beta subunit, and is characterized in that X4 is an amino acid selected from a group comprising cysteine, aspartic acid, glutamic acid, histidine, isoleucine, lysine, methionine, asparagine, proline, glutamine, serine and threonine.Type: GrantFiled: November 30, 2018Date of Patent: November 26, 2019Assignee: Mitsubishi Chemical CorporationInventors: Fumiaki Watanabe, Ai Hara, Takanori Ambo, Aya Kitahara
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Publication number: 20190153518Abstract: Provided are a method with which it is possible to assess the state of periodontal disease and caries by a simple method, a method with which it is possible to assess the severity of periodontal disease, therapeutic effect, and risk of exacerbation by detection of intraoral bacteria, and the like. The present invention is an intraoral examination method that, for example, assesses the state of periodontal disease and/or caries by measuring the levels of bacteria present in an intraoral sample from an intraoral sample collected from a subject.Type: ApplicationFiled: January 9, 2019Publication date: May 23, 2019Applicant: MITSUBISHI CHEMICAL CORPORATIONInventors: Ai HARA, Shinya Murakami, Takenori Nozaki
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Publication number: 20190085313Abstract: Provided is an improved nitrile hydratase with improved catalytic activity. Also provided are DNA for coding the improved nitrile hydratase, a recombinant vector that contains the DNA, a transformant that contains the recombinant vector, nitrile hydratase acquired from a culture of the transformant, and a method for producing the nitrile hydratase. Also provided is a method for producing an amide compound that uses the culture or a processed product of the culture. The improved nitrile hydratase contains an amino acid sequence represented by SEQ ID NO: 50 (GX1X2X3X4DX5X6R) in a beta subunit, and is characterized in that X4 is an amino acid selected from a group comprising cysteine, aspartic acid, glutamic acid, histidine, isoleucine, lysine, methionine, asparagine, proline, glutamine, serine and threonine.Type: ApplicationFiled: November 30, 2018Publication date: March 21, 2019Applicant: Mitsubishi Chemical CorporationInventors: Fumiaki WATANABE, Ai Hara, Takanori Ambo, Aya Kitahara
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Patent number: 10221410Abstract: Provided is an improved nitrile hydratase with improved catalytic activity. Also provided are DNA for coding the improved nitrile hydratase, a recombinant vector that contains the DNA, a transformant that contains the recombinant vector, nitrile hydratase acquired from a culture of the transformant, and a method for producing the nitrile hydratase. Also provided is a method for producing an amide compound that uses the culture or a processed product of the culture. The improved nitrile hydratase contains an amino acid sequence represented by SEQ ID NO: 50 (GX1X2X3X4DX5X6R) in a beta subunit, and is characterized in that X4 is an amino acid selected from a group comprising cysteine, aspartic acid, glutamic acid, histidine, isoleucine, lysine, methionine, asparagine, proline, glutamine, serine and threonine.Type: GrantFiled: June 13, 2018Date of Patent: March 5, 2019Assignee: Mitsubishi Chemical CorporationInventors: Fumiaki Watanabe, Ai Hara, Takanori Ambo, Aya Kitahara
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Publication number: 20180282716Abstract: Provided is an improved nitrile hydratase with improved catalytic activity. Also provided are DNA for coding the improved nitrile hydratase, a recombinant vector that contains the DNA, a transformant that contains the recombinant vector, nitrile hydratase acquired from a culture of the transformant, and a method for producing the nitrile hydratase. Also provided is a method for producing an amide compound that uses the culture or a processed product of the culture. The improved nitrile hydratase contains an amino acid sequence represented by SEQ ID NO: 50 (GX1X2X3X4DX5X6R) in a beta subunit, and is characterized in that X4 is an amino acid selected from a group comprising cysteine, aspartic acid, glutamic acid, histidine, isoleucine, lysine, methionine, asparagine, proline, glutamine, serine and threonine.Type: ApplicationFiled: June 13, 2018Publication date: October 4, 2018Applicant: Mitsubishi Chemical CorporationInventors: Fumiaki WATANABE, Ai Hara, Takanori Ambo, Aya Kitahara
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Patent number: 10066225Abstract: Provided is an improved nitrile hydratase with improved catalytic activity. Also provided are DNA for coding the improved nitrile hydratase, a recombinant vector that contains the DNA, a transformant that contains the recombinant vector, nitrile hydratase acquired from a culture of the transformant, and a method for producing the nitrile hydratase. Also provided is a method for producing an amide compound that uses the culture or a processed product of the culture. The improved nitrile hydratase contains an amino acid sequence represented by SEQ ID NO: 50 (GX1X2X3X4DX5X6R) in a beta subunit, and is characterized in that X4 is an amino acid selected from a group comprising cysteine, aspartic acid, glutamic acid, histidine, isoleucine, lysine, methionine, asparagine, proline, glutamine, serine and threonine.Type: GrantFiled: September 14, 2015Date of Patent: September 4, 2018Assignee: Mitsubishi Chemical CorporationInventors: Fumiaki Watanabe, Ai Hara, Takanori Ambo, Aya Kitahara
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Patent number: 10053732Abstract: A probe or a probe set for evaluating influence of ultraviolet ray on the skin, which includes nucleic acids of (a), (b) or (c): (a) Nucleic acids including a base sequence constituting at least one kind of gene selected from the group consisting of GBA, GLB1, CAT, OLFM1, ASAH1, MMP14, MMP17 and COL18A1, (b) Nucleic acids including a base sequence complementary to the nucleic acids of aforesaid (a); (c) Nucleic acids which hybridize with nucleic acids including a base sequence complementary to the nucleic acids of aforesaid (a) or (b) under stringent conditions, and can detect a skin constitution-related gene.Type: GrantFiled: May 2, 2013Date of Patent: August 21, 2018Assignee: MITSUBISHI CHEMICAL CORPORATIONInventors: Kenjiro Ikuta, Ai Hara, Tatsunobu Fukushima
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Publication number: 20180148769Abstract: The object of the present invention is to provide a bacterial flora analysis method for quantitatively evaluating bacterial flora and to provide a device used in the method. The device relating to the present invention is a device mounted with (a) a probe consisting of a nucleic acid hybridizing to 16SrRNA specific to each of 1 kind or 2 or more kinds of bacteria to be a subject of detection, and at least one of (b) a total amount indicator probe and (c) one or more kinds of an absolute amount indicator probe.Type: ApplicationFiled: November 25, 2016Publication date: May 31, 2018Applicant: Mitsubishi Chemical CorporationInventors: Ai HARA, Hiroyuki OSHIMA
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Patent number: 9738885Abstract: Provided is an improved nitrile hydratase with improved catalytic activity. Also provided are DNA for coding the improved nitrile hydratase, a recombinant vector that contains the DNA, a transformant that contains the recombinant vector, nitrile hydratase acquired from a culture of the transformant, and a method for producing the nitrile hydratase. Also provided is a method for producing an amide compound that uses the culture or a processed product of the culture. The improved nitrile hydratase contains an amino acid sequence represented by SEQ ID NO: 50 (GX1X2X3X4DX5X6R) in a beta subunit, and is characterized in that X4 is an amino acid selected from a group comprising cysteine, aspartic acid, glutamic acid, histidine, isoleucine, lysine, methionine, asparagine, proline, glutamine, serine and threonine.Type: GrantFiled: September 14, 2015Date of Patent: August 22, 2017Assignee: Mitsubishi Chemical CorporationInventors: Fumiaki Watanabe, Ai Hara, Takanori Ambo, Aya Kitahara
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Patent number: 9388403Abstract: Improving wild nitrile hydratase enables the provision of a protein which has nitrile hydratase activity and which has further improved heat resistance, amide compound resistance and high temperature accumulation properties. Use protein (A) or (B), (A) being a protein characterized by having nitrile hydratase activity and by including an amino acid sequence in which a specific amino acid residue in an amino acid sequence in wild nitrile hydratase has been substituted by another amino acid residue, and (B) being a protein characterized by having nitrile hydratase activity and by including an amino acid sequence in which one or several amino acid residues in the amino acid sequence of protein (A), other than the abovementioned specific amino acid residue, is deleted, substituted and/or added.Type: GrantFiled: May 30, 2012Date of Patent: July 12, 2016Assignee: Mitsubishi Rayon Co., Ltd.Inventors: Fumiaki Watanabe, Takanori Ambo, Ai Hara
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Patent number: 9382528Abstract: Improving wild nitrile hydratase enables the provision of a protein which has nitrile hydratase activity and which has further improved heat resistance, amide compound resistance and high temperature accumulation properties. Use protein (A) or (B), (A) being a protein characterised by having nitrile hydratase activity and by including an amino acid sequence in which a specific amino acid residue in an amino acid sequence in wild nitrile hydratase has been substituted by another amino acid residue, and (B) being a protein characterised by having nitrile hydratase activity and by including an amino acid sequence in which one or several amino acid residues in the amino acid sequence of protein (A), other than the abovementioned specific amino acid residue, is deleted, substituted and/or added.Type: GrantFiled: April 17, 2015Date of Patent: July 5, 2016Assignee: Mitsubishi Rayon Co., Ltd.Inventors: Fumiaki Watanabe, Takanori Ambo, Ai Hara
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Publication number: 20160097046Abstract: Provided is an improved nitrile hydratase with improved catalytic activity. Also provided are DNA for coding the improved nitrile hydratase, a recombinant vector that contains the DNA, a transformant that contains the recombinant vector, nitrile hydratase acquired from a culture of the transformant, and a method for producing the nitrile hydratase. Also provided is a method for producing an amide compound that uses the culture or a processed product of the culture. The improved nitrile hydratase contains an amino acid sequence represented by SEQ ID NO: 50 (GX1X2X3X4DX5X6R) in a beta subunit, and is characterized in that X4 is an amino acid selected from a group comprising cysteine, aspartic acid, glutamic acid, histidine, isoleucine, lysine, methionine, asparagine, proline, glutamine, serine and threonine.Type: ApplicationFiled: September 14, 2015Publication date: April 7, 2016Applicant: MITSUBISHI RAYON CO., LTD.Inventors: FUMIAKI WATANABE, Ai HARA, TAKANORI AMBO, AYA KITAHARA
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Publication number: 20160068833Abstract: Provided is an improved nitrile hydratase with improved catalytic activity. Also provided are DNA for coding the improved nitrile hydratase, a recombinant vector that contains the DNA, a transformant that contains the recombinant vector, nitrile hydratase acquired from a culture of the transformant, and a method for producing the nitrile hydratase. Also provided is a method for producing an amide compound that uses the culture or a processed product of the culture. The improved nitrile hydratase contains an amino acid sequence represented by SEQ ID NO: 50 (GX1X2X3X4DX5X6R) in a beta subunit, and is characterized in that X4 is an amino acid selected from a group comprising cysteine, aspartic acid, glutamic acid, histidine, isoleucine, lysine, methionine, asparagine, proline, glutamine, serine and threonine.Type: ApplicationFiled: September 14, 2015Publication date: March 10, 2016Applicant: Mitsubishi Rayon Co., Ltd.Inventors: Fumiaki WATANABE, Ai Hara, Takanori Ambo, Aya Kitahara
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Publication number: 20150337287Abstract: Improving wild nitrile hydratase enables the provision of a protein which has nitrile hydratase activity and which has further improved heat resistance, amide compound resistance and high temperature accumulation properties. Use protein (A) or (B), (A) being a protein characterised by having nitrile hydratase activity and by including an amino acid sequence in which a specific amino acid residue in an amino acid sequence in wild nitrile hydratase has been substituted by another amino acid residue, and (B) being a protein characterised by having nitrile hydratase activity and by including an amino acid sequence in which one or several amino acid residues in the amino acid sequence of protein (A), other than the abovementioned specific amino acid residue, is deleted, substituted and/or added.Type: ApplicationFiled: April 17, 2015Publication date: November 26, 2015Applicant: Mitsubishi Rayon Co., Ltd.Inventors: Fumiaki WATANABE, Takanori Ambo, Ai Hara
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Patent number: 9193966Abstract: Provided is an improved nitrile hydratase with improved catalytic activity. Also provided are DNA for coding the improved nitrile hydratase, a recombinant vector that contains the DNA, a transformant that contains the recombinant vector, nitrile hydratase acquired from a culture of the transformant, and a method for producing the nitrile hydratase. Also provided is a method for producing an amide compound that uses the culture or a processed product of the culture. The improved nitrile hydratase contains an amino acid sequence represented by SEQ ID NO: 50 (GX1X2X3X4DX5X6R) in a beta subunit, and is characterized in that X4 is an amino acid selected from a group comprising cysteine, aspartic acid, glutamic acid, histidine, isoleucine, lysine, methionine, asparagine, proline, glutamine, serine and threonine.Type: GrantFiled: June 7, 2012Date of Patent: November 24, 2015Assignee: Mitsubishi Rayon Co., Ltd.Inventors: Fumiaki Watanabe, Ai Hara, Takanori Ambo, Aya Kitahara
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Publication number: 20150133328Abstract: Provided are a probe or a probe set which can evaluate what the skin condition or a skin cell is, and what kind of influence an external stimulation (particularly ultraviolet ray) to the skin or a skin cell has on the skin condition, such as elasticity and wrinkles, and a nucleic acid microarray which is loaded with the probe or the probe set. The invention is an invention related to a probe or a probe set for evaluating the influence of ultraviolet ray on the skin, which comprises nucleic acids of (a), (b) or (c) described below.Type: ApplicationFiled: May 2, 2013Publication date: May 14, 2015Applicant: MITSUBISHI RAYON CO., LTD.Inventors: Kenjiro Ikuta, Ai Hara, Tatsunobu Fukushima
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Publication number: 20140220644Abstract: Provided is an improved nitrile hydratase with improved catalytic activity. Also provided are DNA for coding the improved nitrile hydratase, a recombinant vector that contains the DNA, a transformant that contains the recombinant vector, nitrile hydratase acquired from a culture of the transformant, and a method for producing the nitrile hydratase. Also provided is a method for producing an amide compound that uses the culture or a processed product of the culture. The improved nitrile hydratase contains an amino acid sequence represented by SEQ ID NO: 50 (GX1X2X3X4DX5X6R) in a beta subunit, and is characterized in that X4 is an amino acid selected from a group comprising cysteine, aspartic acid, glutamic acid, histidine, isoleucine, lysine, methionine, asparagine, proline, glutamine, serine and threonine.Type: ApplicationFiled: June 7, 2012Publication date: August 7, 2014Applicant: Mitsubishi Rayon Co., LtdInventors: Fumiaki Watanabe, Ai Hara, Takanori Ambo, Aya Kitahara