Abstract: The present inventors succeeded in culturing thick three-dimensional muscle tissue in which the muscle fibers are oriented in the same direction by providing channels for transporting a culture medium inside a hydrogel containing skeletal myoblasts. The three-dimensional muscle tissue produced according to the invention is expected to have a steak-like texture. Additionally, in regenerative medicine applications, damage in extensive and thick muscle tissue is expected to be healed with a single treatment by using thick three-dimensional muscle tissue.

Abstract: The present invention provides a production method for a three-dimensional muscle tissue including the steps of: preparing an approximately rectangular first cell module containing skeletal myoblasts in a hydrogel, and having a plurality of approximately rectangular holes parallel to each other, and an approximately rectangular second cell module containing skeletal myoblasts in a hydrogel, and having a plurality of approximately rectangular holes parallel to each other at positions different from those of the first cell module in a vertical direction; alternately stacking the prepared first cell module and the prepared second cell module to obtain a stack; subjecting the skeletal myoblasts contained in the obtained stack to proliferation culture; and inducing the proliferated skeletal myoblasts to differentiate into myotubes.

Abstract: An array includes a plurality of tubes disposed in contact with each other such that axial directions are parallel to each other; and a target substance disposed inside at least one of the plurality of tubes, in which the plurality of tubes are made of anionic hydrogel, and surfaces on which the plurality of tubes are in contact with each other are bonded with an adhesive including nanoparticles having surfaces coated with a cationic water-soluble polymer.