Abstract: The present invention relates to a method of identifying a gene encoding a polypeptide or polypeptide isoform which is substantially more active in either a proanthocyanidin (PA) or anthocyanin (ANT) pathway of a plant, said method including providing material from said plant; and an oligonucleotide probe capable of hybridizing with RNA from a gene encoding a polypeptide which is active in a flavonoid biosynthetic pathway; extracting RNA from said plant material; hybridizing the oligonucleotide probe with the RNA to generate an expression profile; measuring PA and/or ANT levels in said plant material to generate a metabolic profile; comparing said expression profile with said metabolite profile to identify said gene encoding a polypeptide or polypeptide isoform which is substantially active in either a PA or ANT pathway.

Abstract: Genetic constructs capable of manipulating fructan biosynthesis in photosynthetic cells of a plant include a promoter, or functionally active fragment or variant thereof, operatively linked to a nucleic acid encoding a bacterial FT enzyme, or a functionally active fragment or variant thereof. Such constructs can be used in the modification of fructan biosynthesis in plants and, more particularly, to methods of manipulating fructan biosynthesis in photosynthetic cells, for increasing plant biomass and, more particularly, to methods of enhancing biomass yield and/or yield stability, including shoot and/or root growth in a plant, and for enhancing the productivity of biochemical pathways.

Abstract: The present invention relates to nucleic acids encoding flavonoid biosynthetic enzymes, flavonoid-regulating transcription factors and a flavonoid-specific membrane transporter in plants, and the use thereof for the modification of flavonoid biosynthesis in plants. The present invention also relates to constructs and vectors including such nucleic acids, and related polypeptides. More particularly, the protein involved in flavonoid biosynthesis is selected from the group consisting of TRANSPARENT TESTA 12 (TT12), TRANSPARENT TESTA GLABRA 1 (TTG1), TRANSPARENT TESTA 2 (TT2), TRANSPARENT TESTA 8 (TT8), leucoanthocyanidin dioxygenase (LDOX), cinnamate-4-hydroxylase (C4H), 4-coumaroyl:CoA-ligase (4CL); and functionally active fragments and variants thereof.

Abstract: The present invention relates to the modification of fructan biosynthesis in plants and, more particularly, to methods of manipulating fructan biosynthesis in photosynthetic cells, and to related nucleic acids and constructs. The present invention also relates to increasing plant biomass and, more particularly, to methods of enhancing biomass yield and/or yield stability, including shoot and/or root growth in a plant, and to related nucleic acids and constructs. The present invention also relates to methods of enhancing the productivity of biochemical pathways and, more particularly, to fusion proteins in plants, and to related nucleic acids and constructs.

Abstract: The present invention relates to nucleic acids encoding flavonoid biosynthetic enzymes, more particularly flavonoid biosynthesis-regulating transcription factors, and the use thereof for the modification of flavonoid biosynthesis in plants. The present invention also relates to constructs and vectors including such nucleic acids, and related polypeptides. More particularly, the present invention relates to a PURPLE ANTHOCYANIN PIGMENT 1 (PAP1) or PAP1-like transcription factor.

Abstract: The present invention relates to nucleic acids encoding flavonoid biosynthetic enzymes, flavonoid-regulating transcription factors and a flavonoid-specific membrane transporter in plants, and the use thereof for the modification of flavonoid biosynthesis in plants. The present invention also relates to constructs and vectors including such nucleic acids, and related polypeptides. More particularly, the protein involved in flavonoid biosynthesis is selected from the group consisting of TRANSPARENT TESTA 12 (TT12), TRANSPARENT TESTA GLABRA 1 (TTG1), TRANSPARENT TESTA 2 (TT2), TRANSPARENT TESTA 8 (TT8), leucoanthocyanidin dioxygenase (LDOX), cinnamate-4-hydroxylase (C4H), 4-coumaroyl:CoA-ligase (4CL); and functionally active fragments and variants thereof.

Abstract: The present invention relates to the modification of lignin biosynthesis in plants, to enzymes involved in the lignin biosynthetic pathway and nucleic acids encoding such enzymes and, more particularly, to methods of modifying lignin biosynthesis via sense suppression and to related nucleic acids and constructs.

Abstract: Genetic constructs capable of manipulating fructan biosynthesis in photosynthetic cells of a plant, said genetic constructs include a promoter operatively linked to a nucleic acid encoding a bacterial FT enzyme. These constructs can be used in modification of fructan biosynthesis in plants and, more particularly, for manipulating fructan biosynthesis in photosynthetic cells. The constructs can also be used for increasing plant biomass and, more particularly, for enhancing biomass yield and/or yield stability, including shoot and/or root growth in a plant, and for enhancing the productivity of biochemical pathways.

Abstract: The present invention relates to nucleic acids encoding flavonoid biosynthetic enzymes, flavonoid-regulating transcription factors and a flavonoid-specific membrane transporter in plants, and the use thereof for the modification of flavonoid biosynthesis in plants. The present invention also relates to constructs and vectors including such nucleic acids, and related polypeptides. More particularly, the protein involved in flavonoid biosynthesis is selected from the group consisting of: MADS box factor, WRKY box factor, MYC factor, TT1, HLH factor, MYB factor, FMT, UG3E, GST, OMT, RT, CYTb5, laccase, and ABC transporter proteins, and functionally active fragments and variants thereof.

Abstract: The present invention relates to nucleic acids encoding flavonoid biosynthetic enzymes, flavonoid-regulating transcription factors and a flavonoid-specific membrane transporter in plants, and the use thereof for the modification of flavonoid biosynthesis in plants. The present invention also relates to constructs and vectors including such nucleic acids, and related polypeptides. More particularly, the protein involved in flavonoid biosynthesis is selected from the group consisting of TRANSPARENT TESTA 12 (TT12), TRANSPARENT TESTA GLABRA 1 (TTG1), TRANSPARENT TESTA 2 (TT2), TRANSPARENT TESTA 8 (TT8), leucoanthocyanidin dioxygenase (LDOX), cinnamate-4-hydroxylase (C4H), 4-coumaroyl:CoA-ligase (4CL); and functionally active fragments and variants thereof.

Abstract: The present invention relates to nucleic acids encoding flavonoid biosynthetic enzymes, more particularly flavonoid biosynthesis-regulating transcription factors, and the use thereof for the modification of flavonoid biosynthesis in plants. The present invention also relates to constructs and vectors including such nucleic acids, and related polypeptides. More particularly, the present invention relates to a PURPLE ANTHOCYANIN PIGMENT 1 (PAP1) or PAP1-like transcription factor.

Abstract: The present invention relates to the modification of lignin biosynthesis in plants and, more particularly, to enzymes involved in the lignin biosynthetic pathway and nucleic acids encoding such enzymes.

Abstract: The present invention provides methods of transforming plants using electroporation of explants, and methods of producing transgenic plants via electroporation of explants. The methods of the invention are also useful for generating transgenic plants free of marker genes. Also provided are plants produced by the methods of the invention.