Patents by Inventor Aihong Wu

Aihong Wu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20230167196
    Abstract: The invention discloses an anti-phenacetin monoclonal antibody hybridoma cell strain AD, a preparation method and application thereof, and relates to the technical field of food safety immunodetection. The monoclonal antibody hybridoma cell strain is named monoclonal cell strain AD and the number CGMCC19681. The Phe-BA obtained by the hydrolysis of the reaction product of the phenacetin metabolite acetaminophen and ethyl 4-bromobutyrate is used as the hapten, and the hapten is coupled with the carrier protein to prepare the immunogen Phe-BA-BSA. After the mice were immunized with the immunogen Phe-BA-BSA, they were fused with myeloma cells by PEG method, screened by indirect competitive enzyme-linked immunosorbent assay and subcloned five times to obtain hybridoma cell lines. The monoclonal antibody secreted by the cell line can be made into a phenacetin detection kit, which has good affinity and detection sensitivity for phenacetin, and can be used for immunodetection of phenacetin residues in food.
    Type: Application
    Filed: November 9, 2021
    Publication date: June 1, 2023
    Inventors: Chuanlai XU, Jingjing YAO, Hua KUANG, Liguang XU, Maozhong SUN, Xiaoling WU, Liqiang LIU, Wei MA, Jianping ZHU, Changlong HAO, Shanshan SONG, Yongming HU, Aihong WU, Lingling GUO, Xinxin XU
  • Patent number: 10882896
    Abstract: A hybridoma cell strain secreting a nifursol residue marker monoclonal antibody prepared in the following way: BALB/c mice are subjected to the first immunization with a complete Freund's adjuvant, subjected to booster immunization with an incomplete Freund's adjuvant for four times, and subjected to rush immunization once with nifursol residue marker complete antigen without a Freund's adjuvant so that the BALB/c mice are immunized; the spleen cells of the immunized mice with high titer and low IC50 were fused with mouse myeloma cells by a PEG method, and the fused cells are screened through indirect competitive ELISA and subcloned three times. The monoclonal antibody secreted by this cell strain has good specificity and detection sensitivity (IC50 value of 2 ?g/L) to the nifursol residue marker and can be used for residue detection of the nifursol residual marker in food.
    Type: Grant
    Filed: July 21, 2020
    Date of Patent: January 5, 2021
    Assignee: JIANGNAN UNIVERSITY
    Inventors: Hua Kuang, Chuanlai Xu, Aihong Wu, Liguang Xu, Wei Ma, Liqiang Liu, Xiaoling Wu, Shanshan Song, Yongming Hu
  • Publication number: 20200347119
    Abstract: A hybridoma cell strain secreting a nifursol residue marker monoclonal antibody prepared in the following way: BALB/c mice are subjected to the first immunization with a complete Freund's adjuvant, subjected to booster immunization with an incomplete Freund's adjuvant for four times, and subjected to rush immunization once with nifursol residue marker complete antigen without a Freund's adjuvant so that the BALB/c mice are immunized; the spleen cells of the immunized mice with high titer and low IC50 were fused with mouse myeloma cells by a PEG method, and the fused cells are screened through indirect competitive ELISA and subcloned three times. The monoclonal antibody secreted by this cell strain has good specificity and detection sensitivity (IC50 value of 2 ?g/L) to the nifursol residue marker and can be used for residue detection of the nifursol residual marker in food.
    Type: Application
    Filed: July 21, 2020
    Publication date: November 5, 2020
    Inventors: Hua KUANG, Chuanlai XU, Aihong WU, Liguang XU, Wei MA, Liqiang LIU, Xiaoling WU, Shanshan SONG, Yongming HU
  • Patent number: 10759845
    Abstract: A hybridoma cell strain secreting a nifursol residue marker monoclonal antibody with CGMCC No. 18525 is prepared in the following way: BALB/c mice are subjected to the first immunization with a complete Freund's adjuvant, subjected to booster immunization with an incomplete Freund's adjuvant for four times, and subjected to rush immunization once with nifursol residue marker complete antigen without a Freund's adjuvant so that the BALB/c mice are immunized; the spleen cells of the immunized mice with high titer and low IC50 were fused with mouse myeloma cells by a PEG method, and the fused cells are screened through indirect competitive ELISA and subcloned three times. The monoclonal antibody secreted by this cell strain has good specificity and detection sensitivity (IC50 value of 2 ?g/L) to the nifursol residue marker and can be used for residue detection of the nifursol residual marker in food.
    Type: Grant
    Filed: December 17, 2018
    Date of Patent: September 1, 2020
    Inventors: Hua Kuang, Chuanlai Xu, Aihong Wu, Liguang Xu, Wei Ma, Liqiang Liu, Xiaoling Wu, Shanshan Song, Yongming Hu
  • Publication number: 20200048329
    Abstract: A hybridoma cell strain secreting a nifursolol residue marker monoclonal antibody with CGMCC No. 14698 is prepared in the following way: BALB/c mice are subjected to the first immunization with a complete Freund's adjuvant, subjected to booster immunization with an incomplete Freund's adjuvant for four times, and subjected to rush immunization once with nifursolol residue marker complete antigen without a Freund's adjuvant so that the BALB/c mice are immunized; the spleen cells of the immunized mice with high titer and low IC50 were fused with mouse myeloma cells by a PEG method, and the fused cells are screened through indirect competitive ELISA and subcloned three times. The monoclonal antibody secreted by this cell strain has good specificity and detection sensitivity (IC50 value of 2 ?g/L) to the nifursolol residue marker and can be used for residue detection of the nifursolol residual marker in food.
    Type: Application
    Filed: December 17, 2018
    Publication date: February 13, 2020
    Inventors: Hua KUANG, Chuanlai XU, Aihong WU, Liguang XU, Wei MA, Liqiang LIU, Xiaoling WU, Shanshan SONG, Yongming HU
  • Patent number: D852307
    Type: Grant
    Filed: September 29, 2017
    Date of Patent: June 25, 2019
    Inventor: Aihong Wu