Abstract: The present disclosure relates, according to some embodiments, to methods for preparing a library for sequencing. For example, a method may comprise (a) in a coupled reaction, (i) contacting a population of nucleic acid fragments with a tailing enzyme to produce tailed fragments, and (ii) ligating to the tailed fragments a sequencing adapter with a ligase to produce adapter-tagged fragments; and/or separating adapter-tagged fragments from the tailing enzyme and the ligase to produce separated adapter-tagged fragments and, optionally, separated tailing enzyme and/or separated ligase. In some embodiments, a tailing enzyme and/or a ligase used in library preparation may be immobilized enzymes.

Abstract: The present disclosure relates to the catalytic field, and discloses a catalyst system and a light hydrocarbon aromatization method, a carbon dioxide hydrogenation process and a method for enhancing the catalytic activity and/or lifetime of the catalyst during a heterogeneous catalysis process, the catalyst system comprising a porous material layer containing an active metal component and a molecular sieve layer. The catalyst system provided by the present disclosure exhibits desirable catalytic activity, stability, renewability and selectivity, thus has significant benefits.

Abstract: The present invention relates to a detachable pacifier, comprising a limiting device and a pressing device which can be disassembled and assembled with each other, wherein a nipple is arranged below the limiting device and a dustproof cover is arranged outside the pacifier. The limiting device comprises a limiting plate and an annular support portion, an upper edge of the annular support portion is provided with annular clamping portions at intervals; the pressing device comprises a lower end cover, an upper end cover and a pull ring which can be disassembled and assembled with each other. The parts of the detachable pacifier can be disassembled, mounted and cleaned at any time. Further, the clamping blocks at the notch of the annular support portion and a limiting block which is matching the clamping block is provided in the lower end cover, to prevent the detachable pacifier from being disassembled by infants.

Abstract: A catalyst for converting hydrocarbon, a method of making the same, and a method of using the same are provided. Such a catalyst includes a zeotype microporous material, a binder material, and a metal phosphide, which can be in a range of from 0.01% to 10% by weight of a total weight of the catalyst. For example, such a catalyst can be used to convert light alkene or alkane into aromatic hydrocarbon such as benzene, toluene, xylenes, and a combination thereof. The alkene may be ethylene, propylene, butylene, or a combination thereof. The alkene may be supplied directly or from a stream converted from light alkane such as methane, ethane, propane, butane, or a combination thereof.

Abstract: The present disclosure relates, according to some embodiments, to immobilized enzyme compositions and methods for cleaving polynucleotide molecules including, for example, double-stranded DNA. Immobilized enzymes may comprise, for example, an enzyme (e.g., a type IIS restriction endonuclease, an RNAP, a capping enzyme), a support (e.g., a magnetic bead), and optionally, a linker disposed between the enzyme and the support. In some embodiments, methods may include contacting an immobilized enzyme with a polynucleotide substrate to form reaction products, separating the immobilized enzyme from the reaction products, and optionally reusing the immobilized enzymes in one or more subsequent reactions. preparing a library for sequencing.

Abstract: The present disclosure relates to the catalytic field, and discloses a catalyst system and a light hydrocarbon aromatization method, a carbon dioxide hydrogenation process and a method for enhancing the catalytic activity and/or lifetime of the catalyst during a heterogeneous catalysis process, the catalyst system comprising a porous material layer containing an active metal component and a molecular sieve layer. The catalyst system provided by the present disclosure exhibits desirable catalytic activity, stability, renewability and selectivity, thus has significant benefits.

Abstract: Disclosed is a detachable pacifier, comprising a limiting device and a pressing device which can be disassembled and assembled with each other, wherein a nipple is arranged below the limiting device, a dustproof cover is arranged outside the pacifier, the limiting device comprises a limiting plate and an annular support portion extending upwards along the limiting plate, an upper edge of the annular support portion is provided with annular clamping portions at intervals, the pressing device comprises a lower end cover, an upper end cover and a pull ring which can be disassembled and assembled with each other, an upper portion of the lower end cover and the lower end cover body form an annular connecting wall, a groove is arranged in the annular connecting wall in the central symmetrical direction, and the pull ring is arranged in the groove.

Abstract: The present disclosure provides a modified catalyst, and preparation method and a method for producing aromatic hydrocarbons by aromatization of olefins using the modified catalyst. The modified catalyst comprises an acidic molecular sieve and an olefin aromatization active metal component, the total acid amount of the catalyst as measured by NH3-TPD method is not higher than 0.35 mmol/g, and ratio of the strong acid to weak acid is within a range of 0.8-1.2.

Abstract: A cigarette device management system includes an information management module, and a maintenance prediction module, a device state acquisition module and a purchase statistics module which are in communication connection with it. The maintenance prediction module is built by an artificial neural network model and is configured to predict a failure interval. The device state acquisition module acquires a device operating parameter and an operating duration of the device in real time, and compares the device operating parameter with a safety parameter range. In accordance with the system, the maintenance timing of the device can be predicted, the purchase quantity of the spare part and the purchase timing of the spare part also can be predicted.

Abstract: The present disclosure relates, according to some embodiments, to methods for preparing a library for sequencing. For example, a method may comprise (a) in a coupled reaction, (i) contacting a population of nucleic acid fragments with a tailing enzyme to produce tailed fragments, and (ii) ligating to the tailed fragments a sequencing adapter with a ligase to produce adapter-tagged fragments; and/or separating adapter-tagged fragments from the tailing enzyme and the ligase to produce separated adapter-tagged fragments and, optionally, separated tailing enzyme and/or separated ligase. In some embodiments, a tailing enzyme and/or a ligase used in library preparation may be immobilized enzymes.

Abstract: The present disclosure provides a modified catalyst, and preparation method and a method for producing aromatic hydrocarbons by aromatization of olefins using the modified catalyst. The modified catalyst comprises an acidic molecular sieve and an olefin aromatization active metal component, the total acid amount of the catalyst as measured by NH3-TPD method is not higher than 0.35mmo1/g, and ratio of the strong acid to weak acid is within a range of 0.8-1.2.

Abstract: Provided herein is a thermolabile proteinase and methods of using the same. In some embodiments, the thermolabile proteinase may comprise an amino acid sequence that is at least 90% identical to any of SEQ ID NOs:1-11 and at least one amino acid substitution in helix 3. The thermolabile proteinase is active at a temperature in the range of 4° C.-40° C. and is inactivated by raising the temperature to above 50° C., where the proteinase is substantially inactive at 65° C.

Abstract: The present disclosure relates, according to some embodiments, to methods for preparing a library for sequencing. For example, a method may comprise (a) in a coupled reaction, (i) contacting a population of nucleic acid fragments with a tailing enzyme to produce tailed fragments, and (ii) ligating to the tailed fragments a sequencing adapter with a ligase to produce adapter-tagged fragments; and/or separating adapter-tagged fragments from the tailing enzyme and the ligase to produce separated adapter-tagged fragments and, optionally, separated tailing enzyme and/or separated ligase. In some embodiments, a tailing enzyme and/or a ligase used in library preparation may be immobilized enzymes.

Abstract: A catalyst for converting hydrocarbon, a method of making the same, and a method of using the same are provided. Such a catalyst includes a zeotype microporous material, a binder material, and a metal phosphide, which can be in a range of from 0.01% to 10% by weight of a total weight of the catalyst. For example, such a catalyst can be used to convert light alkene or alkane into aromatic hydrocarbon such as benzene, toluene, xylenes, and a combination thereof. The alkene may be ethylene, propylene, butylene, or a combination thereof. The alkene may be supplied directly or from a stream converted from light alkane such as methane, ethane, propane, butane, or a combination thereof.

Abstract: The present disclosure relates, according to some embodiments, to methods for preparing a library for sequencing. For example, a method may comprise (a) in a coupled reaction, (i) contacting a population of nucleic acid fragments with a tailing enzyme to produce tailed fragments, and (ii) ligating to the tailed fragments a sequencing adapter with a ligase to produce adapter-tagged fragments; and/or separating adapter-tagged fragments from the tailing enzyme and the ligase to produce separated adapter-tagged fragments and, optionally, separated tailing enzyme and/or separated ligase. In some embodiments, a tailing enzyme and/or a ligase used in library preparation may be immobilized enzymes.

Abstract: Integrated systems are provided for the production of higher hydrocarbon compositions, for example liquid hydrocarbon compositions, from methane using an oxidative coupling of methane system to convert methane to ethylene, followed by conversion of ethylene to selectable higher hydrocarbon products. Integrated systems and processes are provided that process methane through to these higher hydrocarbon products.

Abstract: The present disclosure provides petrochemical processing methods and systems, including ethylene conversion processes and systems, for the production of higher hydrocarbon compositions, for example liquid hydrocarbon compounds, with reduced amount of unsaturated hydrocarbons.

Abstract: Integrated systems are provided for the production of higher hydrocarbon compositions, for example liquid hydrocarbon compositions, from methane using an oxidative coupling of methane system to convert methane to ethylene, followed by conversion of ethylene to selectable higher hydrocarbon products. Integrated systems and processes are provided that process methane through to these higher hydrocarbon products.

Abstract: Provided herein is a thermolabile proteinase and methods of using the same. In some embodiments, the thermolabile proteinase may comprise an amino acid sequence that is at least 90% identical to any of SEQ ID NOs:1-11 and at least one amino acid substitution in helix 3. The thermolabile proteinase is active at a temperature in the range of 4° C.-40° C. and is inactivated by raising the temperature to above 50° C., where the proteinase is substantially inactive at 65° C.