Abstract: A method for obtaining a transgenic plant that over-expresses a soluble isoform AGPPase enzyme. The method includes a step of transforming a plant with a vector comprising a polynucleotide of SEQ ID NO: 7 linked to a promoter that promotes expression of the polynucleotide in the plant whereby to form the transgenic plant. The transgenic plant has a reduced starch content and a higher resistance to salinity than the plant before the transforming step.

Abstract: Bacterial ADPglucose pyrophosphatase, method of production, use in the manufacture of testing devices and in the production of transgenic plants and bacteria. AGPPase is an enzyme that catalyzes the hydrolysis of ADPG (adenosine diphosphate glucose). The enzyme obtained from microbial extracts is used in testing devices for determining levels of ADPG, based on the G1P (glucose-1-phosphate) released by the reaction catalyzed by AGPPase. Partial amino acid sequences of the enzyme, the sequence of the gene that codes for it and the derived protein are also described. Finally, the production of transgenic plants and bacteria that overexpress the gene of AGPPase is described. The bacteria do not accumulate glycogen, whereas the plants obtained possess a high content of soluble sugars, low starch content and high resistance to high concentrations of salts and to high temperatures.

Abstract: Plant ADPglusose pyrophosphatases, process for production thereof, use in the production of assay devices and in obtaining transgenic plants.