Patents by Inventor Ajith Anand
Ajith Anand has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Publication number: 20240002870Abstract: Methods for transforming monocot leaf explants are provided.Type: ApplicationFiled: September 28, 2021Publication date: January 4, 2024Applicants: PIONEER HI-BRED INTERNATIONAL, INC., CORTEVA AGRISCIENCE LLCInventors: AJITH ANAND, WILLIAM JAMES GORDON-KAMM, LARISA A. RYAN, NAGESH SARDESAI, NING WANG, HUIXIA WU, XINLI EMILY WU
-
Publication number: 20230091338Abstract: Compositions and methods are provided for an inducible, high efficiency intra-genomic homologous recombination (IGHR) system in plants, that can be used for non-chimeric, heritable gene targeting. The advantage of IGHR approach to targeted integration is that every cell contains donor DNA and nuclease-encoding sequences, so that there are many potentially homology-directed targeting events that can occur during plant development.Type: ApplicationFiled: February 22, 2021Publication date: March 23, 2023Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: AJITH ANAND, MAREN L ARLING, PIERLUIGI BARONE, HYEON-JE CHO, WILLIAM JAMES GORDON-KAMM, SANDEEP KUMAR, BRIAN LENDERTS, KEITH S LOWE, SERGEI SVITASHEV, XINLI EMILY WU
-
Patent number: 11330776Abstract: The disclosure pertains to methods and compositions for the rapid and efficient transformation of plants. The disclosure further provides methods for producing a transgenic plant, comprising (a) transforming a cell of an explant with an expression construct comprising (i) a nucleotide sequence encoding a WUS/WOX homeobox polypeptide; (ii) a nucleotide sequence encoding a polypeptide comprising two AP2-DNA binding domains; or (iii) a combination of (i) and (ii); and (b) allowing expression of the polypeptide of (a) in each transformed cell to form a regenerable plant structure in the absence of exogenous cytokinin, wherein no callus is formed; and (c) germinating the regenerable plant structure to form the transgenic plant. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.Type: GrantFiled: August 26, 2016Date of Patent: May 17, 2022Inventors: Ajith Anand, Maren L. Arling, Alexandre Da Silva Conceicao, William James Gordon-Kamm, Craig E Hastings, George J Hoerster, Todd J Jones, Theodore Mitchell Klein, Carlos M La Rota, Keith S Lowe, Shiv Bahadur Tiwari, Ning Wang, Xinli Emily Wu
-
Publication number: 20220124998Abstract: The disclosure pertains to methods and compositions for the rapid and efficient transformation of plants. The disclosure further provides methods for producing a transgenic plant, comprising (a) transforming a cell of an explant with an expression construct comprising (i) a nucleotide sequence encoding a WUS/WOX homeobox polypeptide; (ii) a nucleotide sequence encoding a polypeptide comprising two AP2-DNA binding domains; or (iii) a combination of (i) and (ii); and (b) allowing expression of the polypeptide of (a) in each transformed cell to form a regenerable plant structure in the absence of exogenous cytokinin, wherein no callus is formed; and (c) germinating the regenerable plant structure to form the transgenic plant. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.Type: ApplicationFiled: January 7, 2022Publication date: April 28, 2022Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: AJITH ANAND, MAREN L ARLING, ALEXANDRE DA SILVA CONCEICAO, WILLIAM JAMES GORDON-KAMM, CRAIG HASTINGS, GEORGE J HOERSTER, TODD J JONES, THEODORE M KLEIN, CARLOS M LA ROTA, KEITH S LOWE, SHIV BAHADUR TIWARI, NING WANG, XINLI EMILY WU
-
Patent number: 11236347Abstract: Methods and compositions for Ochrobactrum-mediated transformation of plants are provided. Methods include but are not limited to using an Ochrobactrum strain to transfer a polynucleotide of interest to a plant cell. These include VirD2-dependent methods. Compositions include an Ochrobactrum strain, transfer DNAs, constructs and/or plasmids. These include Ochrobactrum strains having a plasmid comprising one or more virulence gene(s), border region, and/or origin of replication. Plant cells, tissues, plants, and seeds comprising a polynucleotide of interest produced by the methods are also provided.Type: GrantFiled: August 26, 2016Date of Patent: February 1, 2022Inventors: Ajith Anand, Steven Henry Bass, Sean M. Bertain, Hyeon-Je Cho, Anthony J. Kinney, Theodore M. Klein, Michael Lassner, Kevin E. McBride, York Moy, Barbara Ann Marie Rosen, Jun-Zhi Wei
-
Publication number: 20210277409Abstract: Spectinomycin resistant or streptomycin resistant transgenic plants and methods of making such plants are provided.Type: ApplicationFiled: June 25, 2019Publication date: September 9, 2021Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: AJITH ANAND, HYEON-JE CHO, THEODORE M KLEIN
-
Publication number: 20210238614Abstract: Methods and compositions are provided for the improvement of homology-directed repair of a double strand break in a plant cell, via the use of a polynucleotide comprising sequences homologous to the target site. In some aspects, the double strand break is created by an RNA-guided Cas endonuclease. The homology-directed repair of the double-strand break may include incorporation of a heterologous polynucleotide, for example a gene encoding a trait of agronomic importance. The homology-directed repair of the double-strand break may occur as a result of template-directed repair using a polynucleotide repair template.Type: ApplicationFiled: May 7, 2019Publication date: August 5, 2021Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: AJITH ANAND, WILLIAM JAMES GORDON-KAMM, SANDEEP KUMAR, ZHAN-BIN LIU, SERGI SVITASHEV
-
Publication number: 20210062203Abstract: The methods disclosed herein use a morphogenic gene or a repressor gene to provide a transgenic plant containing a single copy of a transgenic event wherein the morphogenic gene or the repressor gene does not integrate into the genome of the plant and the plant does not contain vector (plasmid) backbone.Type: ApplicationFiled: March 11, 2019Publication date: March 4, 2021Applicant: Pioneer Hi-Bred International, Inc.Inventors: Ajith ANAND, William James GORDON-KAMM, George J. HOERSTER, Keith S. LOWE, Kevin E. MCBRIDE
-
Publication number: 20190078106Abstract: The present disclosure provides methods and compositions for the vectors comprising vir genes. The present disclosure further provides a vector comprising: (a) an origin of replication for propagation and stable maintenance in Escherichia coli; (b) an origin of replication for propagation and stable maintenance in Agrobacterium spp.; (c) a selectable marker gene; and (d) Rhizobiaceae virulence genes virB1-B11 or r-virB1-B11, virC1-C2 or r-virC1-C2, virD1-D2 or r-virD1-D2, and virG or r-virG, or variants and derivatives thereof, wherein the vector comprising the virulence genes r-virB1-B11, r-virC1-C2, r-virD1-D2, and r-virG further comprises a r-galls virulence gene, or variants and derivatives thereof. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.Type: ApplicationFiled: August 26, 2016Publication date: March 14, 2019Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: AJITH ANAND, STEVEN HENRY BASS, HYEON-JE CHO, THEODORE MITCHELL KLEIN, MICHAEL LASSNER, KEVIN E. MCBRIDE
-
Publication number: 20180265877Abstract: The present disclosure comprises methods and compositions comprising a plant transforming bacterium of the Order Rhizobiales comprising conditional negative selectable marker genes.Type: ApplicationFiled: October 11, 2016Publication date: September 20, 2018Applicants: PIONEER HI-BRED INTERNATIONAL, INC., E. I. DU PONT DE NEMOURS AND COMPANYInventors: AJITH ANAND, WILLIAM JAMES GORDON-KAMM, LOWE S KEITH, PAMELA L. SHARPE
-
Publication number: 20180216123Abstract: Methods and compositions for Ochrobactrum-mediated transformation of plants are provided. Methods include but are not limited to using an Ochrobactrum strain to transfer a polynucleotide of interest to a plant cell. These include VirD2-dependent methods. Compositions include an Ochrobactrum strain, transfer DNAs, constructs and/or plasmids. These include Ochrobactrum strains having a plasmid comprising one or more virulence gene(s), border region, and/or origin of replication. Plant cells, tissues, plants, and seeds comprising a polynucleotide of interest produced by the methods are also provided.Type: ApplicationFiled: August 26, 2016Publication date: August 2, 2018Applicants: PIONEER HI-BRED INTERNATIONAL, INC., E. I. DU PONT DE NEMOURS AND COMPANYInventors: AJITH ANAND, STEVEN HENRY BASS, SEAN M. BERTAIN, HYEON-JE CHO, ANTHONY J. KINNEY, THEODORE M. KLEIN, MICHAEL LASSNER, KEVIN E. MCBRIDE, YORK MOY, BARBARA ANN MARIE ROSEN, JUN-ZHI WEI
-
Publication number: 20170240911Abstract: Methods and compositions for targeting the insertion of a polynucleotide of interest into a specific chromosomal site in the genome of a plant cell using bacteria-mediated transformation methods are provided. The methods include providing a plant cell with at least one Transfer Cassette comprising in operable linkage a least one polynucleotide construct and at least one recombinase construct, and optionally at least one cell proliferation factor construct. Compositions provided include plant cells, plants or seeds having a polynucleotide of interest inserted at a specific chromosomal site.Type: ApplicationFiled: August 26, 2016Publication date: August 24, 2017Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: AJITH ANAND, WILLIAM JAMES GORDON-KAMM, XINLI EMILY WU
-
Publication number: 20170198266Abstract: Compositions and transformation methods to increase the frequency of plants in a population of transformed plants which have a single copy of the target polynucleotide of interest are provided. The frequency of plants in a population of transformed plants containing no contaminating vector backbone sequence may be increased. The methods and compositions provide for a greater number of transgenic events having single copy inserts and no contaminating vector backbone sequence.Type: ApplicationFiled: June 5, 2015Publication date: July 13, 2017Applicant: Pioneer HI Breed Internaional Inc.Inventors: Ajith Anand, Myeong-Je Cho, William Gordon-Kamm
-
Publication number: 20170121722Abstract: The disclosure pertains to methods and compositions for the rapid and efficient transformation of plants. The disclosure further provides methods for producing a transgenic plant, comprising (a) transforming a cell of an explant with an expression construct comprising (i) a nucleotide sequence encoding a WUS/WOX homeobox polypeptide; (ii) a nucleotide sequence encoding a polypeptide comprising two AP2-DNA binding domains; or (iii) a combination of (i) and (ii); and (b) allowing expression of the polypeptide of (a) in each transformed cell to form a regenerable plant structure in the absence of exogenous cytokinin, wherein no callus is formed; and (c) germinating the regenerable plant structure to form the transgenic plant. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.Type: ApplicationFiled: August 26, 2016Publication date: May 4, 2017Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: AJITH ANAND, MAREN L. ARLING, ALEXANDRE DA SILVA CONCEICAO, WILLIAM JAMES GORDON-KAMM, CRAIG E. HASTINGS, GEORGE M. HOERSTER, THEODORE MITCHELL KLEIN, CARLOS M. LA ROTA, KEITH S. LOWE, SHIV BAHADUR TIWARI, NING WANG, XINLI EMILY WU
-
Patent number: 8053638Abstract: The invention provides methods and compositions for enhancing the efficiency of Agrobacterium-mediated transformation of host cells such as plant cells. Plant expression constructs comprising a gene encoding a VIP2 or VIP2-like polypeptide are provided, as well as methods for utilizing such constructs to enhance Agrobacterium-mediated transformation efficiency.Type: GrantFiled: August 7, 2007Date of Patent: November 8, 2011Assignee: The Samuel Roberts Noble FoundationInventors: Ajith Anand, Kirankumar Mysore
-
Publication number: 20090271896Abstract: The invention provides methods and compositions for enhancing the efficiency of Agrobacterium-mediated transformation of host cells such as plant cells. Plant expression constructs comprising a gene encoding a VIP2 or VIP2-like polypeptide are provided, as well as methods for utilizing such constructs to enhance Agrobacterium-mediated transformation efficiency.Type: ApplicationFiled: August 7, 2007Publication date: October 29, 2009Inventors: Ajith Anand, Kirankumar Mysore