Abstract: Provided is a method for measuring an anti-drug antibody that can be performed more simply and inexpensively than conventional methods. Provided is a double antigen bridging immunoassay using a capture nucleic acid and a tracer nucleic acid. By using the capture nucleic acid and the tracer nucleic acid in the double antigen bridging immunoassay, an anti-drug antibody can be measured simply and inexpensively. Furthermore, by using the tracer nucleic acid, it becomes possible to adopt a high sensitivity detection method utilizing a nucleic acid.

Abstract: The present invention provides a method of detecting and a method of quantifying oligonucleotides with more excellent specificity and quantitativity as compared to conventional signal amplification (PALSAR) measurement methods. In the present invention, the problem is solved by hybridizing a target oligonucleotide to be measured with a complementary nucleic acid probe (3?-complementary sequence of target sequence-5?), or hybridizing the target oligonucleotide to be measured having given bases such as poly(A) added thereto with a complementary nucleic acid probe (3?-complementary sequence of target oligonucleotide+complementary sequence of given bases-5?), decomposing and removing an incomplete hybridization product by using a single-strand-specific nuclease such as Si nuclease, and measuring the nucleic acid probe contained in a remaining complete hybridization product by a PALSAR method.

Abstract: An object of the present invention is to provide a more sensitive detection method and quantification method in a method for detecting a target nucleic acid using the PALSAR method.

Abstract: The present invention provides a method of detecting and a method of quantifying oligonucleotides with more excellent specificity and quantitativity as compared to conventional signal amplification (PALSAR) measurement methods. In the present invention, the problem is solved by hybridizing a target oligonucleotide to be measured with a complementary nucleic acid probe (3?-complementary sequence of target sequence-5?), or hybridizing the target oligonucleotide to be measured having given bases such as poly(A) added thereto with a complementary nucleic acid probe (3?-complementary sequence of target oligonucleotide+complementary sequence of given bases-5?), decomposing and removing an incomplete hybridization product by using a single-strand-specific nuclease such as Si nuclease, and measuring the nucleic acid probe contained in a remaining complete hybridization product by a PALSAR method.