Patents by Inventor Aki Iguchi

Aki Iguchi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 9359647
    Abstract: A polymorphism-detecting probe, an amplification primer and the use thereof are provided to enable simple and highly reliable determination of different polymorphisms in an EGFR gene.
    Type: Grant
    Filed: January 27, 2014
    Date of Patent: June 7, 2016
    Assignee: ARKRAY, Inc.
    Inventors: Aki Iguchi, Moeko Ijuin
  • Patent number: 9169518
    Abstract: The invention provides a primer set for detecting a polymorphism in EGFR exon 21 L858R. The primer set has a P1 oligonucleotide and a P2 oligonucleotide and can performing amplification by using a region including the 172792nd base of SEQ ID NO: 1 as a template. As a base that is complementary to the 172792nd base of SEQ ID NO: 1, the P1 oligonucleotide has cytosine and the P2 oligonucleotide has adenine. The melting temperature of the P1 oligonucleotide is higher than the melting temperature of the P2 oligonucleotide, and/or the P1 oligonucleotide is one or more bases longer than the P2 oligonucleotide. The invention further provides a polymorphism detection primer, a polymorphism detection method using the primer set, a method of evaluating a EGFR tyrosine kinase inhibitor using the primer set, a primer used in the polymorphism detection method, and a kit including the primer set.
    Type: Grant
    Filed: October 28, 2011
    Date of Patent: October 27, 2015
    Assignee: ARKRAY, Inc.
    Inventor: Aki Iguchi
  • Patent number: 9115391
    Abstract: The present invention provides a method for detecting a mutation capable of detecting a mutation with high sensitivity and high reliability in one reaction system. Using primers (Xmt) and (Xwt), a target nucleic acid sequence whose objective base to be detected is a mutant-type is amplified with amplification efficiency higher than a target nucleic acid sequence whose objective base to be detected is a normal-type. The (Xmt) is a primer that is complementary to a region including a mutant-type base in the template nucleic acid and has a base complementary to a mutant-type base at a 3? region, and the (Xwt) is a primer that is complementary to a region including a normal-type base in the template nucleic acid and has a base complementary to a normal-type base at a 3? region. It is preferable that amplification efficiency by the (Xmt) with reference to a mutant-type template nucleic acid is higher than that by the (Xwt) with reference to a normal-type template nucleic acid.
    Type: Grant
    Filed: July 2, 2009
    Date of Patent: August 25, 2015
    Assignee: ARKRAY, Inc.
    Inventors: Mitsuharu Hirai, Toshiya Hosomi, Aki Iguchi
  • Patent number: 9096894
    Abstract: The present invention provides a dry reagent capable of amplifying a nucleic acid even after being stored in a dry state at room temperature, a dry reagent kit, a reagent container, and a method for producing the dry reagent and methods of producing and using thereof.
    Type: Grant
    Filed: May 29, 2012
    Date of Patent: August 4, 2015
    Assignee: ARKRAY, Inc.
    Inventors: Aki Iguchi, Mariko Komori
  • Patent number: 9045799
    Abstract: Provided in the present disclosure is a probe for detecting polymorphism that enables a simple detection of polymorphism in the CYP3A gene with high sensitivity.
    Type: Grant
    Filed: March 9, 2012
    Date of Patent: June 2, 2015
    Assignee: ARKRAY, Inc.
    Inventors: Aki Iguchi, Mitsuharu Hirai
  • Publication number: 20140170657
    Abstract: A polymorphism-detecting probe, an amplification primer and the use thereof are provided to enable simple and highly reliable determination of different polymorphisms in an EGFR gene.
    Type: Application
    Filed: January 27, 2014
    Publication date: June 19, 2014
    Applicant: ARKRAY, INC.
    Inventors: AKI IGUCHI, MOEKO IJUIN
  • Patent number: 8748590
    Abstract: An oligonucleotide for a detection test of a polymorphism of EGFR exon 19, the oligonucleotide being at least one selected from the group consisting of a P1 oligonucleotide and a P1? oligonucleotide, the P1 oligonucleotide having a 3? end subjected to an extension inhibition treatment, which has an identity of at least 80% with respect to a base sequence including at least the 115th to the 123rd bases of the base sequence indicated in SEQ ID NO: 1 and has a length of from 9 to 80 bases; and the P1? oligonucleotide having a 3? end subjected to an extension inhibition treatment, which hybridizes under stringent conditions with a complementary strand of a base sequence including at least the 115th to the 123rd bases of the base sequence indicated in SEQ ID NO: 1 and having a length of from 9 to 80 bases.
    Type: Grant
    Filed: May 1, 2012
    Date of Patent: June 10, 2014
    Assignee: ARKRAY, Inc.
    Inventor: Aki Iguchi
  • Patent number: 8624017
    Abstract: A probe for detecting polymorphism in the ABCG2 gene is constituted by including, for example, an oligonucleotide which is complementary to a base sequence including the 301st to the 311th bases of the base sequence indicated in SEQ ID NO:1 and having a length of from 11 bases to 50 bases, and has an identity of at least 80%, and in which a base corresponding to the 311th base has been labeled with a fluorescent dye.
    Type: Grant
    Filed: April 27, 2012
    Date of Patent: January 7, 2014
    Assignee: ARKRAY, Inc.
    Inventors: Aki Iguchi, Kaoru Kurose
  • Patent number: 8624018
    Abstract: A probe for detecting polymorphism in the ABCC2 gene is constituted by including, for example, an oligonucleotide which is complementary to a base sequence including the 207th to the 217th bases of the base sequence indicated in SEQ ID NO:1 and having a length of from 11 bases to 60 bases, and has an identity of at least 80%, and in which a base corresponding to the 217th base has been labeled with a fluorescent dye.
    Type: Grant
    Filed: April 27, 2012
    Date of Patent: January 7, 2014
    Assignee: ARKRAY, Inc.
    Inventor: Aki Iguchi
  • Publication number: 20130029861
    Abstract: The present disclosure includes a method for simultaneously detecting a plurality of nucleotide polymorphisms, comprising detecting a plurality of nucleotide polymorphisms at a single wavelength by using a plurality of oligonucleotides labeled with a dye, each of which hybridizes to a region containing each of the plurality of nucleotide polymorphisms.
    Type: Application
    Filed: May 31, 2012
    Publication date: January 31, 2013
    Applicant: ARKRAY, Inc.
    Inventor: Aki Iguchi
  • Publication number: 20120301943
    Abstract: The present invention provides a dry reagent capable of amplifying a nucleic acid even after being stored in a dry state at room temperature, a dry reagent kit, a reagent container, and a method for producing the dry reagent and methods of producing and using thereof.
    Type: Application
    Filed: May 29, 2012
    Publication date: November 29, 2012
    Applicant: ARKRAY, INC.
    Inventors: AKI IGUCHI, MARIKO KOMORI
  • Publication number: 20120288859
    Abstract: The present disclosure relates to probes for detecting a polymorphism of HGF gene.
    Type: Application
    Filed: May 9, 2012
    Publication date: November 15, 2012
    Inventors: Aki Iguchi, Mitsuharu Hirai
  • Publication number: 20120282610
    Abstract: An oligonucleotide for a detection test of a polymorphism of EGFR exon 19, the oligonucleotide being at least one selected from the group consisting of a P1 oligonucleotide and a P1? oligonucleotide, the P1 oligonucleotide having a 3? end subjected to an extension inhibition treatment, which has an identity of at least 80% with respect to a base sequence including at least the 115th to the 123rd bases of the base sequence indicated in SEQ ID NO: 1 and has a length of from 9 to 80 bases; and the P1? oligonucleotide having a 3? end subjected to an extension inhibition treatment, which hybridizes under stringent conditions with a complementary strand of a base sequence including at least the 115th to the 123rd bases of the base sequence indicated in SEQ ID NO: 1 and having a length of from 9 to 80 bases.
    Type: Application
    Filed: May 1, 2012
    Publication date: November 8, 2012
    Inventor: Aki Iguchi
  • Publication number: 20120282608
    Abstract: A probe for detecting polymorphism in the ABCC2 gene is constituted by including, for example, an oligonucleotide which is complementary to a base sequence including the 207th to the 217th bases of the base sequence indicated in SEQ ID NO:1 and having a length of from 11 bases to 60 bases, and has an identity of at least 80%, and in which a base corresponding to the 217th base has been labeled with a fluorescent dye.
    Type: Application
    Filed: April 27, 2012
    Publication date: November 8, 2012
    Inventor: Aki Iguchi
  • Publication number: 20120282609
    Abstract: A gene mutation detection probe for detection of a target gene mutation in a base sequence encoding a gene of interest that includes the target gene mutation and a non-target gene mutation, the probe including at least one oligonucleotide selected from the group consisting of oligonucleotides P1, P1-1, P1?, and P 1?-1.
    Type: Application
    Filed: April 27, 2012
    Publication date: November 8, 2012
    Inventors: Mitsuharu Hirai, Kaoru Kurose, Aki Iguchi
  • Publication number: 20120282607
    Abstract: A probe for detecting polymorphism in the ABCG2 gene is constituted by including, for example, an oligonucleotide which is complementary to a base sequence including the 301st to the 311th bases of the base sequence indicated in SEQ ID NO:1 and having a length of from 11 bases to 50 bases, and has an identity of at least 80%, and in which a base corresponding to the 311th base has been labeled with a fluorescent dye.
    Type: Application
    Filed: April 27, 2012
    Publication date: November 8, 2012
    Inventors: Aki Iguchi, Kaoru Kurose
  • Publication number: 20120276533
    Abstract: A probe for detection of at least 1 type of genetic polymorphism of the ALDH2 gene rs671 and the ADH2 gene rs1229984, a kit therefore, and methods of detecting the polymorphism(s).
    Type: Application
    Filed: April 27, 2012
    Publication date: November 1, 2012
    Applicant: ARKRAY, Inc.
    Inventors: Mitsuharu Hirai, Aki Iguchi
  • Publication number: 20120270215
    Abstract: Provided in the present disclosure is a probe for detecting polymorphism that enables a simple detection of polymorphism in the CYP3A gene with high sensitivity.
    Type: Application
    Filed: March 9, 2012
    Publication date: October 25, 2012
    Inventors: Aki Iguchi, Mitsuharu Hirai
  • Publication number: 20120252024
    Abstract: The present invention provides probes that can identify different polymorphisms in a c-kit gene easily with high reliability and use thereof.
    Type: Application
    Filed: December 13, 2010
    Publication date: October 4, 2012
    Applicant: ARKRAY, Inc.
    Inventors: Mariko Komori, Aki Iguchi
  • Publication number: 20120107816
    Abstract: The invention provides a primer set for detecting a polymorphism in EGFR exon 21 L858R. The primer set has a P1 oligonucleotide and a P2 oligonucleotide and can performing amplification by using a region including the 172792nd base of SEQ ID NO: 1 as a template. As a base that is complementary to the 172792nd base of SEQ ID NO: 1, the P1 oligonucleotide has cytosine and the P2 oligonucleotide has adenine. The melting temperature of the P1 oligonucleotide is higher than the melting temperature of the P2 oligonucleotide, and/or the P1 oligonucleotide is one or more bases longer than the P2 oligonucleotide. The invention further provides a polymorphism detection primer, a polymorphism detection method using the primer set, a method of evaluating a EGFR tyrosine kinase inhibitor using the primer set, a primer used in the polymorphism detection method, and a kit including the primer set.
    Type: Application
    Filed: October 28, 2011
    Publication date: May 3, 2012
    Applicant: ARKRAY, Inc.
    Inventor: Aki Iguchi