Patents by Inventor Aki Tomiki
Aki Tomiki has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230399632Abstract: The present invention relates to glucoamylase variants having improved thermostability and compositions comprising such variants. The present invention further relates to polynucleotides encoding such variants, vectors and host cells comprising genes encoding such variants, which may also enable the production of such variants. The present invention also relates to methods of liquefying starch-containing materials using or applying the variants or compositions, as well as the saccharification thus produced by the method. The present invention also relates to methods of saccharifying starch-containing materials using or applying the variants or compositions, as well as the saccharides thus produced by the method. The present invention further relates to processes for producing fermentation products from starch-containing or cellulosic-containing material, as well as an enzyme blend or composition, or a recombinant host cell or fermenting organism suitable for use in processes of the invention.Type: ApplicationFiled: November 2, 2021Publication date: December 14, 2023Applicant: Novozymes A/SInventors: Yuma Kurakata, Aki Tomiki-Hashizume
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Patent number: 11447763Abstract: The present invention relates to protease variants, having improved properties compared to the parent protease, in particular variants of a serine protease belonging to family 53 derived from a strain of Meripilus giganteus. The variants according to the invention have in particular increased thermo-stability, e.g., increased residual activity after 30 min at a temperature in the range from 55 to 60° C. and/or increased thermal denaturation temperature, compared to the parent Meripilus giganteus protease. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: GrantFiled: January 14, 2021Date of Patent: September 20, 2022Assignee: NOVOZYMES A/SInventors: Keiichi Ayabe, Tomoko Matsui, Aki Tomiki, Yuma Kurakata, Esben P. Friis, Jens E. Nielsen, Roland Alexander Pache
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Patent number: 11427811Abstract: The present invention relates to a variant pullulanase, wherein the pullulanase comprises at least the following combination of substitutions: N368G+N393A+Q431E+L432F+A492A,S+N610R+G624S+T631S+S632C, and optionally further comprises N222P+Q252A+Q256R; wherein the variant has pullulanase activity, and wherein the variants have at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide of SEQ ID NO: 3. Further aspect the present invention relates to a process for liquefying starch-containing material at a temperature above the initial gelatinization temperature using an alpha-amylase and a thermo-stable pullulanase of the invention.Type: GrantFiled: July 11, 2016Date of Patent: August 30, 2022Assignee: Novozymes A/SInventors: Tomoko Matsui, Suzanne Clark, Aki Tomiki
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Publication number: 20220154226Abstract: The present invention relates to a variant pullulanase, having increased thermo-stability and/or increased thermo-activity compared to a parent pullulanase, comprising a substitution at least a one position selected from a position corresponding to positions 432, 486, 370, 17, 77, 103, 106, 107, 190, 196, 197, 262, 279, 283, 321, 367, 375, 382, 399, 401, 402, 411, 412, 434, 435, 443, 446, 459, 460, 479, 490, 498, 514, 529, 531, 533, 541, 545, 581, 583, 595, 649, 665, 688, 700, 709, 804, 811 of SEQ ID NO: 1, and optionally a deletion of one or more, e.g., all amino acids at positions 821, 822, 823, 824, 825, 826, 827, and 828, wherein the variant has pullulanase activity, and wherein the variant has at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to a parent alpha amylase selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5.Type: ApplicationFiled: March 17, 2020Publication date: May 19, 2022Applicant: Novozymes A/SInventors: Takashi Nakanishi, Aki Tomiki, Yuma Kurakata
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Publication number: 20220090041Abstract: The present invention relates to protease variants, having improved properties compared to the parent protease, in particular variants of a serine protease belonging to family 53 derived from a strain of Meripilus giganteus. The variants according to the invention have in particular increased thermo-stability, e.g., increased residual activity after 30 min at a temperature in the range from 55 to 60° C. and/or increased thermal denaturation temperature, compared to the parent Meripilus giganteus protease. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: December 9, 2021Publication date: March 24, 2022Applicant: Novozymes A/SInventors: Keiichi Ayabe, Tomoko Matsui, Aki Tomiki-Hashizume, Yuma Kurakata, Esben Peter Friis, Jens Erik Nielsen, Roland Alexander Pache
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Publication number: 20210139876Abstract: The present invention relates to protease variants, having improved properties compared to the parent protease, in particular variants of a serine protease belonging to family 53 derived from a strain of Meripilus giganteus. The variants according to the invention have in particular increased thermo-stability, e.g., increased residual activity after 30 min at a temperature in the range from 55 to 60° C. and/or increased thermal denaturation temperature, compared to the parent Meripilus giganteus protease. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: January 14, 2021Publication date: May 13, 2021Applicant: NOVOZYMES A/SInventors: Keiichi Ayabe, Tomoko Matsui, Aki Tomiki, Yuma Kurakata, Esben P. Friis, Jens E. Nielsen, Roland Alexander Pache
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Patent number: 10927361Abstract: The present invention relates to protease variants, having improved properties compared to the parent protease, in particular variants of a serine protease belonging to family 53 derived from a strain of Meripilus giganteus. The variants according to the invention have in particular increased thermo-stability, e.g., increased residual activity after 30 min at a temperature in the range from 55 to 60° C. and/or increased thermal denaturation temperature, compared to the parent Meripilus giganteus protease. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: GrantFiled: July 14, 2017Date of Patent: February 23, 2021Assignee: NOVOZYMES A/SInventors: Keiichi Ayabe, Tomoko Matsui, Aki Tomiki, Yuma Kurakata, Esben P. Friis, Jens E. Nielsen, Roland Alexander Pache
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Publication number: 20190352629Abstract: The present invention relates to protease variants, having improved properties compared to the parent protease, in particular variants of a serine protease belonging to family 53 derived from a strain of Meripilus giganteus. The variants according to the invention have in particular increased thermo-stability, e.g., increased residual activity after 30 min at a temperature in the range from 55 to 60° C. and/or increased thermal denaturation temperature, compared to the parent Meripilus giganteus protease. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: July 14, 2017Publication date: November 21, 2019Applicant: NOVOZYMES A/SInventors: Keiichi Ayabe, Tomoko Matsui, Aki Tomiki, Yuma Kurakata, Esben Peter Friis, Jens Erik Nielsen, Roland Alexander Pache
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Patent number: 10472617Abstract: The present invention relates to asparaginase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. The invention further relates to a process of producing a fermentation product, comprising: liquefying a starch-containing material to dextrins with an alpha-amylase in the presence of an asparaginase of the invention; saccharifying the dextrins to a sugar with a glucoamylase; and fermenting the sugar using a fermenting organism.Type: GrantFiled: September 25, 2017Date of Patent: November 12, 2019Assignee: Novozymes A/SInventors: Tomoko Matsui, Aki Tomiki, Allan Svendsen, Hanne Vang Hendriksen, Mary Ann Stringer, Keiichi Ayabe
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Publication number: 20180208917Abstract: The present invention relates to a variant pullulanase, wherein the pullulanase comprises at least the following combination of substitutions: N368G+N393A+Q431E+L432F+A492A,S+N610R+G624S+T631S+S632C, and optionally further comprises N222P+Q252A+Q256R; wherein the variant has pullulanase activity, and wherein the variants have at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide of SEQ ID NO: 3. Further aspect the present invention relates to a process for liquefying starch-containing material at a temperature above the initial gelatinization temperature using an alpha-amylase and a thermo-stable pullulanase of the invention.Type: ApplicationFiled: July 11, 2016Publication date: July 26, 2018Applicant: NOVOZYMES A/SInventors: Tomoko Matsui, Suzanne Clark, Aki Tomiki
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Publication number: 20180030428Abstract: The present invention relates to asparaginase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. The invention further relates to a process of producing a fermentation product, comprising: liquefying a starch-containing material to dextrins with an alpha-amylase in the presence of an asparaginase of the invention; saccharifying the dextrins to a sugar with a glucoamylase; and fermenting the sugar using a fermenting organism.Type: ApplicationFiled: September 25, 2017Publication date: February 1, 2018Applicant: Novozymes A/SInventors: Tomoko Matsui, Aki Tomiki, Allan Svendsen, Hanne Vang Hendriksen, Mary Ann Stringer, Keiichi Ayabe
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Patent number: 9828595Abstract: The present invention relates to asparaginase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. The invention further relates to a process of producing a fermentation product, comprising: liquefying a starch-containing material to dextrins with an alpha-amylase in the presence of an asparaginase of the invention; saccharifying the dextrins to a sugar with a glucoamylase; and fermenting the sugar using a fermenting organism.Type: GrantFiled: August 15, 2013Date of Patent: November 28, 2017Assignee: Novozymes A/SInventors: Tomoko Matsui, Aki Tomiki, Allan Svendsen, Hanne Vang Hendriksen, Mary Ann Stringer, Keiichi Ayabe
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Patent number: 9765316Abstract: The present invention relates to variants of alpha amylase. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: GrantFiled: January 11, 2016Date of Patent: September 19, 2017Assignee: NOVOZYMES A/SInventors: Tomoko Matsui, Aki Tomiki, Guillermo Coward-Kelly
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Publication number: 20160130572Abstract: The present invention relates to variants of alpha amylase. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: January 11, 2016Publication date: May 12, 2016Applicant: NOVOZYMES A/SInventors: Tomoko Matsui, Aki Tomiki, Guillermo Coward-Kelly
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Patent number: 9267124Abstract: The present invention relates to variants of alpha amylase. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: GrantFiled: July 6, 2012Date of Patent: February 23, 2016Assignee: NOVOZYMES A/SInventors: Tomoko Matsui, Aki Tomiki, Guillermo Coward-Kelly
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Publication number: 20150210995Abstract: The present invention relates to asparaginase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. The further relates to a process of producing a fermentation product, comprising: liquefying a starch-containing material to dextrins with an alpha-amylase in the presence of an asparaginase of the invention; saccharifying the dextrins to a sugar with a glucoamylase; and fermenting the sugar using a fermenting organism.Type: ApplicationFiled: August 15, 2013Publication date: July 30, 2015Inventors: Tomoko Matsui, Aki Tomiki, Allan Svendsen, Hanne Vang Hendriksen, Mary Ann Stringer, Keiichi Ayabe
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Publication number: 20140147893Abstract: The present invention relates to variants of alpha amylase. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: July 6, 2012Publication date: May 29, 2014Applicant: NOVOZYMES A/SInventors: Tomoko Matsui, Aki Tomiki, Guillermo Coward-Kelly