Patents by Inventor Akihiko Kondo

Akihiko Kondo has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20210171935
    Abstract: The present invention provides a method of modifying a targeted site of a double stranded DNA, including a step of contacting a complex wherein a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a selected double stranded DNA and DNA glycosylase with sufficiently low reactivity with a DNA having an unrelaxed double helix structure (unrelaxed DNA) are bonded, with the double stranded DNA, to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site, without cleaving at least one strand of the double stranded DNA in the targeted site.
    Type: Application
    Filed: February 12, 2021
    Publication date: June 10, 2021
    Applicant: National University Corporation Kobe University
    Inventors: Keiji NISHIDA, Akihiko KONDO
  • Patent number: 10920215
    Abstract: The present invention provides a method of modifying a targeted site of a double stranded DNA, including a step of contacting a complex wherein a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a selected double stranded DNA and DNA glycosylase with sufficiently low reactivity with a DNA having an unrelaxed double helix structure (unrelaxed DNA) are bonded, with the double stranded DNA, to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site, without cleaving at least one strand of the double stranded DNA in the targeted site.
    Type: Grant
    Filed: November 2, 2015
    Date of Patent: February 16, 2021
    Assignee: National University Corporation Kobe University
    Inventors: Keiji Nishida, Akihiko Kondo
  • Publication number: 20210017528
    Abstract: One or more embodiments of the present invention are to provide a new means of improving the productivity of a target protein. The present inventors have identified a novel protein consisting of the amino acid sequence set forth in SEQ ID NO: 2 through an exhaustive analysis of the nucleotide sequence of chromosomal DNA of a yeast belonging to the genus Komagataella. Activation of a gene encoding the novel protein provides a cell having an improved productivity of a target protein.
    Type: Application
    Filed: September 25, 2020
    Publication date: January 21, 2021
    Applicants: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY, KANEKA CORPORATION
    Inventors: Yoichiro Ito, Jun Ishii, Yasuyuki Nakamura, Akihiko Kondo, Teruyuki Nishi
  • Publication number: 20200377910
    Abstract: The present invention provides a method of modifying a targeted site of a double-stranded DNA, comprising a step of introducing a complex wherein a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a double-stranded DNA and PmCDA1 are bonded, into a cell containing the double-stranded DNA, and culturing the cell at a low temperature at least temporarily to convert the targeted site, i.e., the target nucleotide sequence and nucleotides in the vicinity thereof, to other nucleotides, or delete the targeted site, or insert nucleotide into the site.
    Type: Application
    Filed: April 21, 2017
    Publication date: December 3, 2020
    Applicant: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY
    Inventors: Keiji NISHIDA, Akihiko KONDO, Takayuki ARAZOE, Zenpei SHIMATANI
  • Patent number: 10767173
    Abstract: The invention provides a method of modifying a targeted site of gram-positive bacterium of a double stranded DNA. The method includes contacting the double-stranded DNA with a complex of a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a given double stranded DNA and a nucleic acid base converting enzyme to convert, delete, or insert one or more nucleotides in the targeted site without cleaving at least one strand of the double stranded DNA in the targeted site, by introducing the nucleic acid encoding the complex into the gram-positive bacterium. The invention also provide a nucleic acid-modifying enzyme complex of a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a double stranded DNA of a gram-positive bacterium and a nucleic acid base converting enzyme bonded to each other, which complex is used for the method.
    Type: Grant
    Filed: September 9, 2016
    Date of Patent: September 8, 2020
    Assignees: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY, NIPPON SHOKUBAI CO., LTD.
    Inventors: Masaharu Mukoyama, Eita Ichige, Keiji Nishida, Akihiko Kondo
  • Publication number: 20200248174
    Abstract: The invention provides a method of modifying a targeted site of a double stranded DNA, including a step of contacting a complex wherein a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a selected double stranded DNA and a nucleic acid base converting enzyme are linked, with the double stranded DNA, to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site, without cleaving at least one strand of the double stranded DNA in the targeted site.
    Type: Application
    Filed: April 2, 2020
    Publication date: August 6, 2020
    Inventors: Keiji Nishida, Akihiko Kondo, Satomi Kojima
  • Publication number: 20200208138
    Abstract: The invention provides a method of modifying a targeted site of a double stranded DNA, including a step of contacting a complex wherein a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a selected double stranded DNA and a nucleic acid base converting enzyme are linked, with the double stranded DNA, to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site, without cleaving at least one strand of the double stranded DNA in the targeted site.
    Type: Application
    Filed: February 14, 2020
    Publication date: July 2, 2020
    Inventors: Keiji Nishida, Akihiko Kondo, Satomi Kojima
  • Patent number: 10655123
    Abstract: The invention provides a method of modifying a targeted site of a double stranded DNA, including a step of contacting a complex wherein a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a selected double stranded DNA and a nucleic acid base converting enzyme are linked, with the double stranded DNA, to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site, without cleaving at least one strand of the double stranded DNA in the targeted site.
    Type: Grant
    Filed: March 4, 2015
    Date of Patent: May 19, 2020
    Assignee: National University Corporation Kobe University
    Inventors: Keiji Nishida, Akihiko Kondo, Satomi Kojima
  • Publication number: 20200010856
    Abstract: Provided is a method for altering a targeted site of a DNA in a cell, including a step of stimulating the cell with a factor inducing a DNA modifying enzyme endogenous to the cell, and bringing a complex of a nucleic acid sequence-recognizing module specifically binding to a target nucleotide sequence in a given DNA and a DNA modifying enzyme-binding module bonded to each other into contact with the DNA to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site.
    Type: Application
    Filed: March 20, 2018
    Publication date: January 9, 2020
    Applicant: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY
    Inventors: Keiji NISHIDA, Akihiko KONDO, Takayuki ARAZOE, Shin YOSHIOKA
  • Publication number: 20190345527
    Abstract: The object of the present invention is to provide a method for synthesizing a double-stranded DNA fragment using a PCR method, the method being capable of accurately, easily, efficiently, and quickly synthesizing a double-stranded DNA fragment of interest, regardless of the sequence thereof. The present invention is a method for synthesizing a double-stranded DNA by connecting short double-stranded DNAs to each other by overlap extension PCR to obtain a double-stranded DNA fragment of interest, the method having a plurality of stages of annealing temperature setting in a PCR cycle of the overlap extension PCR.
    Type: Application
    Filed: November 9, 2018
    Publication date: November 14, 2019
    Inventors: Kenji TSUGE, Shunsuke TAKAHASHI, Akihiko KONDO
  • Patent number: 10351882
    Abstract: There is provided a method for generating an oil/fat component, in which salt-tolerant algae are cultured in a culture medium of which the salt concentration has been increased in a stepwise manner.
    Type: Grant
    Filed: March 17, 2014
    Date of Patent: July 16, 2019
    Assignees: National University Corporation KOBE University, Inter-University Research Institute Corporation National Institutes of Natural Sciences, DIC Corporation
    Inventors: Akihiko Kondo, Tomohisa Hasunuma, Shih-hsin Ho, Jun Minagawa, Haruo Nishie, Hiroyuki Taroda, Jo-Shu Chang
  • Publication number: 20190203198
    Abstract: The invention provides a method of modifying a targeted site of gram-positive bacterium of a double stranded DNA. The method includes contacting the double-stranded DNA with a complex of a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a given double stranded DNA and a nucleic acid base converting enzyme to convert, delete, or insert one or more nucleotides in the targeted site without cleaving at least one strand of the double stranded DNA in the targeted site, by introducing the nucleic acid encoding the complex into the gram-positive bacterium. The invention also provide a nucleic acid-modifying enzyme complex of a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a double stranded DNA of a gram-positive bacterium and a nucleic acid base converting enzyme bonded to each other, which complex is used for the method.
    Type: Application
    Filed: September 9, 2016
    Publication date: July 4, 2019
    Applicants: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY, NIPPON SHOKUBAI CO., LTD.
    Inventors: Masaharu MUKOYAMA, Eita ICHIGE, Keiji NISHIDA, Akihiko KONDO
  • Publication number: 20190085342
    Abstract: The present invention provides a method of modifying a targeted site of a double stranded DNA of a monocot cell, comprising a step of contacting a complex wherein a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in the given double stranded DNA and a nucleic acid base converting enzyme are bonded, with said double stranded DNA, to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into said targeted site, without cleaving at least one strand of said double stranded DNA in the targeted site, wherein the double stranded DNA is contacted with the complex by introducing a nucleic acid encoding the complex into the monocot cell.
    Type: Application
    Filed: November 25, 2016
    Publication date: March 21, 2019
    Applicant: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY
    Inventors: Keiji NISHIDA, Zenpei SHIMATANI, Akihiko KONDO
  • Patent number: 10214756
    Abstract: There is provided a method for generating an oil/fat component by means of culturing algae, in which marine algae belonging to chlamydomonas are cultured in a culture medium containing sea salt.
    Type: Grant
    Filed: March 17, 2014
    Date of Patent: February 26, 2019
    Assignees: National University Corporation KOBE University, Inter-University Research Institute Corporation National Institutes of Natural Sciences, DIC Corporation
    Inventors: Akihiko Kondo, Tomohisa Hasunuma, Shih-hsin Ho, Jun Minagawa, Haruo Nishie, Hiroyuki Taroda, Jo-Shu Chang
  • Publication number: 20190024098
    Abstract: The present invention provides a method of modifying a targeted site of a double stranded DNA in a host cell, the method including introducing (a) a DNA encoding a crRNA containing a sequence complementary to a target strand of a target nucleotide sequence in the given double stranded DNA, and (b) a DNA encoding a protein group constituting Cascade and a nucleic acid base converting enzyme, in which the nucleic acid base converting enzyme is constituted in a form capable of forming a complex with any protein in the protein group, into the host cell to convert one or more nucleotides in the targeted site to other one or more nucleotides, or delete one or more nucleotides, or insert one or more nucleotides into said targeted site, without cleaving the double stranded DNA in the targeted site.
    Type: Application
    Filed: September 8, 2016
    Publication date: January 24, 2019
    Applicant: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY
    Inventors: Keiji NISHIDA, Satomi KOJIMA, Akihiko KONDO
  • Publication number: 20190002931
    Abstract: The production method of the present invention includes: a first fermentation step of adding at least one microorganism selected from yeast, Escherichia coli, and bacteria of the genus Corynebacterium to a first concentrated sugar solution to ferment the first concentrated sugar solution and subjecting the resulting first fermented solution to solid-liquid separation, the first concentrated sugar solution being obtained by using a nanofiltration membrane and/or a reverse osmosis membrane to concentrate a sugar solution obtained using a herbaceous plant of the family Gramineae or Cucurbitaceae as a raw material; and a second fermentation step of adding a second concentrated sugar solution to a solid component obtained in the first fermentation step to ferment the second concentrated sugar solution with the microorganism used in the first fermentation step, and subjecting the resulting second fermented solution to solid-liquid separation.
    Type: Application
    Filed: August 10, 2016
    Publication date: January 3, 2019
    Applicants: NITTO DENKO CORPORATION, NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY
    Inventors: Kengo SASAKI, Chiaki OGINO, Akihiko KONDO, Toshiyuki KAWASHIMA, Masahiko HIROSE, Takahisa KONISHI
  • Publication number: 20180044710
    Abstract: The present invention aims to provide a yeast that is genetically modified so as to more highly produce glutathione, and a method of producing glutathione utilizing the yeast. The present invention provides a method of producing glutathione, including culturing a yeast whose thiol oxidase activity is increased as compared to the parent strain in a culture medium to produce glutathione, and recovering glutathione from the cultured broth obtained.
    Type: Application
    Filed: March 4, 2016
    Publication date: February 15, 2018
    Applicants: KANEKA CORPORATION, NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY
    Inventors: Kiyotaka HARA, Akihiko KONDO, Akira IWASAKI, Yuichi IWAMOTO
  • Publication number: 20170321210
    Abstract: The present invention provides a method of modifying a targeted site of a double stranded DNA, including a step of contacting a complex wherein a nucleic acid sequence-recognizing module that specifically binds to a target nucleotide sequence in a selected double stranded DNA and DNA glycosylase with sufficiently low reactivity with a DNA having an unrelaxed double helix structure (unrelaxed DNA) are bonded, with the double stranded DNA, to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site, without cleaving at least one strand of the double stranded DNA in the targeted site.
    Type: Application
    Filed: November 2, 2015
    Publication date: November 9, 2017
    Applicant: National University Corporation Kobe University
    Inventors: Keiji NISHIDA, Akihiko KONDO
  • Patent number: 9751917
    Abstract: Disclosed is a polynucleotide for cell surface expression. The polynucleotide of the invention comprises a promoter, a secretion signal sequence, a sequence encoding an intended protein, and a sequence encoding a cell surface-localized protein or a cell membrane-binding domain thereof, wherein the promoter is a promoter of a gene encoding the cell surface-localized protein. Provided is a polynucleotide for cell surface expression allowing the production of yeast displaying an enzyme on the cell surface with a high activity.
    Type: Grant
    Filed: March 25, 2014
    Date of Patent: September 5, 2017
    Assignee: National University Corporation Kobe University
    Inventors: Akihiko Kondo, Tomohisa Hasunuma, Kentaro Inokuma
  • Publication number: 20170218382
    Abstract: An expression vector is disclosed which contains a promoter DNA; a DNA encoding a peptide having a defined amino acid sequence and having secretion signal activity; and a DNA encoding an intended protein or a cloning site for insertion of the DNA encoding an intended protein. An expression vector is also disclosed which contains a promoter DNA; a DNA encoding any peptide having a defined amino acid sequence and having secretion signal activity; a DNA encoding an intended protein or a cloning site for insertion of the DNA encoding an intended protein; and a DNA encoding an anchor domain. The peptide having secretion signal activity allows for secretory production and cell surface display of a protein with high activity, in yeast.
    Type: Application
    Filed: July 30, 2015
    Publication date: August 3, 2017
    Applicant: National University Corporation Kobe University
    Inventors: Akihiko KONDO, Tomohisa HASUNUMA, Kentaro INOKUMA