Abstract: A method comprising liquefying a gas to thereby obtain a liquefied gas; injecting a sample into a mobile phase containing a solvent and a supercritical fluid formed from the liquefied gas; passing this mobile phase through a column so that the mobile phase containing desired substance is divided into the solvent and the gas; and separating the desired substance from the solvent, wherein when the pressure of the gas divided from the mobile phase is higher than the pressure of the gas fed for formation of the liquefied gas from gas supply device, the gas divided from the mobile phase is liquefied. Further, there is provided a vapor liquid separator for use in the method.

Abstract: A method comprising liquefying a gas to thereby obtain a liquefied gas; injecting a sample into a mobile phase containing a solvent and a supercritical fluid formed from the liquefied gas; passing this mobile phase through a column so that the mobile phase containing desired substance is divided into the solvent and the gas; and separating the desired substance from the solvent, wherein when the pressure of the gas divided from the mobile phase is higher than the pressure of the gas fed for formation of the liquefied gas from gas supply device, the gas divided from the mobile phase is liquefied. Further, there is provided a vapor liquid separator for use in the method.

Abstract: A method comprising liquefying a gas to thereby obtain a liquefied gas; injecting a sample into a mobile phase containing a solvent and a supercritical fluid formed from the liquefied gas; passing this mobile phase through a column so that the mobile phase containing desired substance is divided into the solvent and the gas; and separating the desired substance from the solvent, wherein when the pressure of the gas divided from the mobile phase is higher than the pressure of the gas fed for formation of the liquefied gas from gas supply device, the gas divided from the mobile phase is liquefied. Further, there is provided a vapor liquid separator for use in the method.

Abstract: A supercritical fluid chromatography using a column using a column having an optical isomer separating agent containing a polysaccharide derivative capable of optical isomer separation, wherein use is made of a mobile phase containing a supercritical fluid and wherein as the optical isomer separating agent received in the column to conduct optical isomer separation, an optical separating agent containing a polysaccharide derivative capable of optical isomer separation in an amount of 50% by mass or more based on the entirety of the optical isomer separating agent is used to thereby, even in the use of optical isomer separating agent with a multiplicity of identification sites, enable accomplishing excellent separation of optical isomers.

Abstract: A method comprising liquefying a gas to thereby obtain a liquefied gas; injecting a sample into a mobile phase containing a solvent and a supercritical fluid formed from the liquefied gas; passing this mobile phase through a column so that the mobile phase containing desired substance is divided into the solvent and the gas; and separating the desired substance from the solvent, wherein when the pressure of the gas divided from the mobile phase is higher than the pressure of the gas fed for formation of the liquefied gas from gas supply device, the gas divided from the mobile phase is liquefied. Further, there is provided a vapor liquid separator for use in the method.

Abstract: In a chromatography using a pressurized mobile phase, the mobile phase flowing through a feed pipe (31) is introduced into one end of a bypass pipe (32) where a sample is sealed through a two-way valve (v2), a two-way valve (v3) is opened and a two-way valve (v1) is closed when the sample is pressurized by the introduction of the mobile phase, all the pressurized sample is thereby injected into the feed pipe (31), and further the pressure variation of the mobile phase in the feed pipe is suppressed when the sample is injected.