Abstract: A method for producing lipid particles, including: injecting molten lipids directly into a liquid at a temperature lower than a melting point of the lipids through a liquid supply port of a two-fluid nozzle while injecting a gas directly into the liquid through a gas supply port of the two-fluid nozzle, so that the molten lipids are dispersed and atomized into particles in the liquid due to the gas and the particles are solidified to form lipid particles. The lipids have a water solubility of 10 g/L or less at 25° C. and are solid at 25° C. The two-fluid nozzle is heated to a temperature at least 10° C. higher than the melting point of the lipids. A ratio D50/Nd of a volume median diameter D50 of the lipid particles to an orifice diameter Nd of the liquid supply port of the two-fluid nozzle is 0.0017 or more and 0.17 or less.

Abstract: Stable and economical methods for producing coenzyme Q10A are described. These methods include concentrating a culture suspension of a coenzyme Q10-producing microorganism efficiently, while minimizing a loss of coenzyme Q10 prior to an extraction step or a homogenization process. The methods for producing coenzyme Q10 may comprise a filtration step of passing a culture suspension of a coenzyme Q10-producing microorganism through a porous membrane in a state where the culture suspension is heated to a heating temperature of 35° C. or higher. In order to improve the average permeation flux, a regenerating step may be performed, preferably at least once, which involves closing a filtration device, applying pressure, and then releasing the pressure; or a regeneration treatment of allowing a medium to flow in from a filtrate outlet side and passing the medium through the porous membrane for a certain time, prior to normal filtration.

Abstract: A method of producing coenzyme Q10 includes contacting an extract from a coenzyme Q10-producing microorganism with an adsorbent (A) such that the adsorbent (A) adsorbs a component of the extract other than coenzyme Q10, and that coenzyme Q10 is obtained. The adsorbent (A) includes aluminum silicate at a content of 50% or more.

Abstract: A method of producing coenzyme Q10 includes contacting an extract from a coenzyme Q10-producing microorganism with an adsorbent (A) such that the adsorbent (A) adsorbs a component of the extract other than coenzyme Q10, and that coenzyme Q10 is obtained. The adsorbent (A) includes aluminum silicate at a content of 50% or more.

Abstract: A method of producing coenzyme Q10 includes contacting an extract from a coenzyme Q10-producing microorganism with an adsorbent (A) such that the adsorbent (A) adsorbs a component of the extract other than coenzyme Q10, and that coenzyme Q10 is obtained. The adsorbent (A) includes aluminum silicate at a content of 50% or more.

Abstract: A process for producing on an industrial scale the oxidized coenzyme Q10, includes culturing a reduced coenzyme Q10-producing microorganism selected from the group consisting of the genus Rhodobacter, the genus Saitoella, the genus Schizosaccharomyces and the genus Trichosporon, to obtain microbial cells containing reduced coenzyme Q10 at a ratio of not less than 70 mole % among the entire coenzymes Q10; and one of: (a) oxidizing thus-obtained reduced coenzyme Q10 to oxidized coenzyme Q10 and then extracting the oxidized coenzyme Q10 by an organic solvent; or (b) extracting reduced coenzyme Q10 by an organic solvent and oxidizing the extracted reduced coenzyme Q10 to oxidized coenzyme Q10.

Abstract: A process for producing on an industrial scale the oxidized coenzyme Q10, includes culturing a reduced coenzyme Q10-producing microorganism selected from the group consisting of the genus Rhodobacter, the genus Saitoella, the genus Schizosaccharomyces and the genus Trichosporon, to obtain microbial cells containing reduced coenzyme Q10 at a ratio of not less than 70 mole % among the entire coenzymes Q10; and one of: (a) oxidizing thus-obtained reduced coenzyme Q10 to oxidized coenzyme Q10 and then extracting the oxidized coenzyme Q10 by an organic solvent; or (b) extracting reduced coenzyme Q10 by an organic solvent and oxidizing the extracted reduced coenzyme Q10 to oxidized coenzyme Q10.

Abstract: The present invention relates to a process for producing reduced coenzyme Q10 which comprises obtaining microbial cells containing reduced coenzyme Q10 at a ratio of not less than 70 mole % among the entire coenzymes Q10, optionally disrupting the cells and recovering thus produced reduced coenzyme Q10. The present invention also relates to a process for producing oxidized coenzyme Q10 which comprises either recovering oxidized coenzyme Q10 after oxidizing the above-mentioned microbial cells or disrupted product thereof, or recovering reduced coenzyme Q10 from the above-mentioned microbial cells or disrupted product thereof to oxidize thus-obtained reduced coenzyme Q10 thereafter. According to the processes of the present invention, reduced coenzyme Q10 and oxidized coenzyme Q10 can be produced simply on the industrial scale.

Abstract: Provided is a production method of a lipophilic bioactive substance, which includes mixing an aqueous suspension of a microbial cell containing the lipophilic bioactive substance or a microbial cell homogenate thereof and an organic solvent in the presence of a particular surfactant, and extracting the lipophilic bioactive substance into the organic solvent phase. This production method enables extraction without using special dehydrating, drying facility, and without causing a decrease in the yield due to degraded separability between solvent and fungus body component, as well as efficient industrial production.

Abstract: Provided is a production method of a lipophilic bioactive substance, which includes mixing an aqueous suspension of a microbial cell containing the lipophilic bioactive substance or a microbial cell homogenate thereof and an organic solvent in the presence of a particular surfactant, and extracting the lipophilic bioactive substance into the organic solvent phase. This production method enables extraction without using special dehydrating, drying facility, and without causing a decrease in the yield due to degraded separability between solvent and fungus body component, as well as efficient industrial production.

Abstract: A method for production of fine microcapsules which encapsulate a hydrophilic bioactive substance at a high content and can be used in wide range of applications such as foods and medical drugs, which method enabling efficient industrial production, is provided.

Abstract: The objective of the present invention is to provide a method for easily producing a porous cellulose particle which can be used as an adsorbent for various substances in a safe manner without using a highly toxic solvent such as a calcium thiocyanate solution. In addition, the objective of the present invention is to provide a porous particle produced by the production method, an adsorbent which contains the porous particle and by which a highly pure protein can be efficiently purified in a safe manner, and a method for purifying a protein by using the adsorbent.

Abstract: The present invention relates to a process for producing reduced coenzyme Q10 which comprises obtaining microbial cells containing reduced coenzyme Q10 at a ratio of not less than 70 mole % among the entire coenzymes Q10, optionally disrupting the cells and recovering thus produced reduced coenzyme Q10. The present invention also relates to a process for producing oxidized coenzyme Q10 which comprises either recovering oxidized coenzyme Q10 after oxidizing the above-mentioned microbial cells or disrupted product thereof, or recovering reduced coenzyme Q10 from the above-mentioned microbial cells or disrupted product thereof to oxidize thus-obtained reduced coenzyme Q10 thereafter. According to the processes of the present invention, reduced coenzyme Q10 and oxidized coenzyme Q10 can be produced simply on the industrial scale.

Abstract: The present invention relates to a process for producing reduced coenzyme Q10 which comprises obtaining microbial cells containing reduced coenzyme Q10 at a ratio of not less than 70 mole % among the entire coenzymes Q10, optionally disrupting the cells and recovering thus-produced reduced coenzyme Q10. The present invention also relates to a process for producing oxidized coenzyme Q10 which comprises either recovering oxidized coenzyme Q10 after oxidizing the above-mentioned microbial cells or disrupted product thereof, or recovering reduced coenzyme Q10 from the above-mentioned microbial cells or disrupted product thereof to oxidize thus-obtained reduced coenzyme Q10 thereafter. According to the processes of the present invention, reduced coenzyme Q10 and oxidized coenzyme Q10 can be produced simply on the industrial scale.

Abstract: A method for production of fine microcapsules which encapsulate a hydrophilic bioactive substance at a high content and can be used in wide range of applications such as foods and medical drugs, which method enabling efficient industrial production, is provided.

Abstract: The present invention relates to a process for producing reduced coenzyme Q10 which comprises obtaining microbial cells containing reduced coenzyme Q10 at a ratio of not less than 70 mole % among the entire coenzymes Q10, optionally disrupting the cells and recovering thus-produced reduced coenzyme Q10. The present invention also relates to a process for producing oxidized coenzyme Q10 which comprises either recovering oxidized coenzyme Q10 after oxidizing the above-mentioned microbial cells or disrupted product thereof, or recovering reduced coenzyme Q10 from the above-mentioned microbial cells or disrupted product thereof to oxidize thus-obtained reduced coenzyme Q10 thereafter. According to the processes of the present invention, reduced coenzyme Q10 and oxidized coenzyme Q10 can be produced simply on the industrial scale.

Abstract: The present invention relates to a process for producing reduced coenzyme Q10 which comprises obtaining microbial cells containing reduced coenzyme Q10 at a ratio of not less than 70 mole % among the entire coenzymes Q10, optionally disrupting the cells and recovering thus-produced reduced coenzyme Q10. The present invention also relates to a process for producing oxidized coenzyme Q10 which comprises either recovering oxidized coenzyme Q10 after oxidizing the above-mentioned microbial cells or disrupted product thereof, or recovering reduced coenzyme Q10 from the above-mentioned microbial cells or disrupted product thereof to oxidize thus-obtained reduced coenzyme Q10 thereafter. According to the processes of the present invention, reduced coenzyme Q10 and oxidized coenzyme Q10 can be produced simply on the industrial scale.