Abstract: Provided is a botulinum toxin producing method which is simple achieves a high toxin yield, and obtains a toxin having high specific activity. This botulinum toxin producing method includes: (A) a step in which a botulinum toxin is produced from botulinum toxin-producing bacteria in a medium, and a mixture a is obtained which contains a botulinum toxin, a bacterial component, and a nucleic acid component derived from the botulinum toxin; (B) a step in which the mixture a is subjected to the removal of the bacterial component, and a mixture b is obtained which contains a nucleic acid component and a botulinum toxin; (C) a step in which an endonuclease is added to the mixture b and a mixture c is obtained which contains a nucleic acid degradation product and a botulinum toxin; and (D) a step in which the mixture c is subjected to removal of the nucleic acid degradation product, and an isolated botulinum toxin liquid d is obtained.

Abstract: A cooling control apparatus controls a plurality of cooling members. The apparatus has a monitor which monitors states of predetermined combinations of the cooling members and a controller which controls the cooling members based on the states monitored by the monitor.

Abstract: A portable valuables receptacle is disclosed which includes a mechanism for prevention of slippage of the receptacle out of a garment pocket in which it is positioned, and which further prevents removal thereof from the pocket by a pickpocket. A slide plate provided with a portion for reception of valuables is slidably mounted within a case and movable between two positions, one being a locked position and the second being a released position. In the released position the slide plate is moved outwardly of the case by spring action, thus causing the case and the slide plate to expand against the side margins of the garment pocket in which it is placed, thus placing a force against the side margins and thereby preventing removal thereof from the garment pocket. The valuables receptacle portion of the slide plate is arranged to receive valuable personal belongings such as a commutation ticket, driver's license, a key or the like.