Abstract: The method of staining a biological sample includes: a first liquid application step of applying a first liquid onto one of a biological sample or a substrate on which the biological sample is placed, the first liquid containing a water-repellent component and being aqueous; a second liquid application step of applying a second liquid onto one of the biological sample or the substrate, the second liquid containing a thickening component and being aqueous; and a staining solution application step of applying a staining solution for staining the biological sample. The first liquid and the second liquid are applied so as to overlap each other at least partially, to thereby produce a mixed liquid. The first liquid application step and the second liquid application step include applying the first liquid and the second liquid so that the mixed liquid surrounds a region for holding the staining solution.

Abstract: Provided is a staining method by which a ligand protein having specificity for a target in a biological sample can be stably applied in an intended amount to an intended site through ejection by an inkjet system. The staining method includes ejecting, from a liquid ejection head of an inkjet system, a liquid composition containing a ligand protein having specificity for a target in a biological sample, and a surfactant represented by the following formula (1), to thereby apply the liquid composition to the biological sample.

Abstract: An aqueous ink that enables recording of an image with suppressed feathering and has high ejection stability can be provided. An aqueous ink for ink jet contains a pigment, a polyhydric alcohol derivative and a water-soluble organic solvent. The polyhydric alcohol derivative is a compound in which a proportion of {Y/(X+Y)}×100 (%) where X mole(s) of ethylene oxide group(s) and Y mole(s) of propylene oxide group(s) are added to polyhydric alcohol having three or more hydroxy groups is 55% or more to 95% or less. The water-soluble organic solvent includes a first water-soluble organic solvent having a relative dielectric constant of 10.0 or more to 40.0 or less.

Abstract: Provided is an ink jet recording method for recording an image on a recording medium by making use of an aqueous reaction liquid containing a reactant and first and second inks which are aqueous inks containing a coloring material. This method has a step of applying the reaction liquid to a first recording medium, a step of applying the first and second inks, in order of mention, to the first recording medium to form a first image and a step of bringing a porous layer of a liquid absorption member into contact with the first image and thereby absorbing a liquid component therefrom. The second image has brightness higher than that of the first ink.

Abstract: An ink jet recording method for recording an image on a recording medium by using an aqueous reaction liquid containing a reactant and a first ink and a second ink, each a water-based ink containing a coloring material, includes a reaction liquid applying step, an image formation step for forming a first image by applying the first ink and the second ink in this order to the transfer body so as to overlap at least partially with a region to which the reaction liquid has been applied, a liquid absorption step for bringing a porous layer possessed by a liquid absorption member into contact with the first image to absorb a liquid component therefrom, and after the liquid absorption step, a transfer step for transferring the first image on the transfer body to the recording medium, and the first ink has brightness higher than the brightness of the second ink.

Abstract: Provided is an ink jet recording method for recording an image on a recording medium by making use of an aqueous reaction liquid containing a reactant and first and second inks which are aqueous inks containing a coloring material. This method has a step of applying the reaction liquid to a first recording medium, a step of applying the first and second inks, in order of mention, to the first recording medium to form a first image and a step of bringing a porous layer of a liquid absorption member into contact with the first image and thereby absorbing a liquid component therefrom. The second image has brightness higher than that of the first ink.

Abstract: An aqueous ink that enables recording of an image with suppressed feathering and has high ejection stability can be provided. An aqueous ink for ink jet contains a pigment, a polyhydric alcohol derivative and a water-soluble organic solvent. The polyhydric alcohol derivative is a compound in which a proportion of {Y/(X+Y)}×100 (%) where X mole(s) of ethylene oxide group(s) and Y mole(s) of propylene oxide group(s) are added to polyhydric alcohol having three or more hydroxy groups is 55% or more to 95% or less. The water-soluble organic solvent includes a first water-soluble organic solvent having a relative dielectric constant of 10.0 or more to 40.0 or less.

Abstract: An image recording method including an ink application step of applying an ink containing a coloring material, an anionic water-soluble resin, resin particles and an inclusion compound to a recording medium; and a first liquid composition application step of applying a first liquid composition containing at least one of an organic acid and a polyvalent metal ion to the recording medium so as to at least partly overlap with an area where the ink is applied.

Abstract: A liquid composition including a fluorinated nonionic surfactant and a water-soluble inclusion compound. The liquid composition can be used for forming images on a recording medium.

Abstract: An image recording method including an ink application step of applying an ink containing a coloring material, an anionic water-soluble resin, resin particles and an inclusion compound to a recording medium; and a first liquid composition application step of applying a first liquid composition containing at least one of an organic acid and a polyvalent metal ion to the recording medium so as to at least partly overlap with an area where the ink is applied.

Abstract: An image recording method includes recording an intermediate image by applying an ink to an intermediate transfer body, and transferring the intermediate image onto a recording medium while heating the intermediate image. The ink contains a coloring material and polymer fine particles which have an interpenetrating polymer network structure constituted by a crystalline polymer and an amorphous polymer. In transferring the intermediate image, the intermediate image is heated to a temperature equal to or higher than a melting point of the crystalline polymer and equal to or higher than a glass transition point of the amorphous polymer.

Abstract: A charge controlling agent is provided as a compound in which an amide group is introduced together with a sulfonic group or a derivative thereof. The charge controlling agent is characterized by including at least one compound represented by the chemical formula (2) in which an amide group is introduced together with at least one of a sulfonic group, a sulfonic acid ester, a sulfonic acid salt, and a sulfonic acid halide.

Abstract: There is provided a polymer including a unit represented by Chemical Formula (1): wherein R represents -A1-SO2R1; R1w and R1x are each independently a halogen atom or a hydrogen atom; R1y is a CH3 group, a halogen atom or a hydrogen atom; A01 is a substituted or unsubstituted aromatic ring structure or a substituted or unsubstituted heterocyclic structure; A1 is a substituted or unsubstituted aliphatic hydrocarbon structure, a substituted or unsubstituted aromatic ring structure or a substituted or unsubstituted heterocyclic structure; R1 is OH, a halogen atom, ONa, OK or OR1a; R1a is a substituted or unsubstituted aliphatic hydrocarbon structure, a substituted or unsubstituted aromatic ring structure or a substituted or unsubstituted heterocyclic structure.

Abstract: There is provided a polymer including a unit represented by Chemical Formula (1): wherein R represents -A1-SO2R1; R1w and R1X are each independently a halogen atom or a hydrogen atom; R1y is a CH3 group, a halogen atom or a hydrogen atom; A01 is a substituted or unsubstituted aromatic ring structure or a substituted or unsubstituted heterocyclic structure; A1 is a substituted or unsubstituted aliphatic hydrocarbon structure, a substituted or unsubstituted aromatic ring structure or a substituted or unsubstituted heterocyclic structure; R1 is OH, a halogen atom, ONa, OK or OR1a; R1a is a substituted or unsubstituted aliphatic hydrocarbon structure, a substituted or unsubstituted aromatic ring structure or a substituted or unsubstituted heterocyclic structure.

Abstract: A charge controlling agent is provided as a compound in which an amide group is introduced together with a sulfonic group or a derivative thereof. The charge controlling agent is characterized by including at least one compound represented by the chemical formula (2) in which an amide group is introduced together with at least one of a sulfonic group, a sulfonic acid ester, a sulfonic acid salt, and a sulfonic acid halide.

Abstract: A polycarbonate resin composition comprising substantially:94.0-99.5% by weight of a polycarbonate resin, and0.5-6.0% by weight of zeolite having tannic acid adsorbed thereon.In this polycarbonate resin composition, the molecular weight reduction of polycarbonate resin is suppressed.