Patents by Inventor Akimitsu Okamoto
Akimitsu Okamoto has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240009224Abstract: The present invention provides a nucleic acid having excellent cell membrane permeability.Type: ApplicationFiled: September 1, 2023Publication date: January 11, 2024Applicants: The University of Tokyo, AGC Inc.Inventors: Takashi OKAZOE, Kohsuke AIKAWA, Honoka WATANABE, Akimitsu OKAMOTO, Kunihiko MORIHIRO, Taiichi KAGEYAMA
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Publication number: 20230242554Abstract: It is an object of the present invention to provide a compound that has absorption in a near infrared region, has high efficiency of singlet oxygen generation (quantum yield), and also has high tumor toxicity when it is combined with an immunotoxin. According to the present invention, provided is a compound represented by the following formula (1) or a salt thereof: wherein L1 and L2 each independently represent a single bond, —O—, —CO—, an alkylene group containing 1 to 8 carbon atoms, a sugar chain, or a combination thereof; R1 and R2 each independently represent an alkyl group containing 1 to 8 carbon atoms, a carboxylic acid group, an amino group, a hydroxyl group, a thiol group, or a biotin residue; R3, R4, R5, R6, R7 and R8 each independently represent an alkyl group containing 1 to 8 carbon atoms, a phenyl group, a carboxylic acid group, an amino group, a hydroxyl group, a thiol group, or a biotin residue; and M represents Mg, Zn, Fe, P, Si, Cu, Sn, Al, Ti, Mo, or Ni.Type: ApplicationFiled: June 24, 2021Publication date: August 3, 2023Applicants: The University of Tokyo, PhotoQ3 Inc.Inventors: Akimitsu OKAMOTO, Wakako DEWA, Takao HAMAKUBO, Mikako HAMABE, Naoko TODA, Yukio SUDO
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Publication number: 20220235091Abstract: To provide a nucleic acid excellent in cell membrane permeability and a method for its production. A nucleic acid containing a C2-10 perfluoroalkyl group having from 1 to 5 ether-bonding oxygen atoms between carbon atoms; said nucleic acid wherein said perfluoroalkyl group is directly or indirectly bonded to the 5?- or 3?-end of the nucleic acid, or said perfluoroalkyl group is indirectly introduced between two nucleotides; or a nucleic acid medicine comprising, as an active ingredient, a nucleic acid containing a C2-10 perfluoroalkyl group, which may have from 1 to 5 ether-bonding oxygen atoms between carbon atoms.Type: ApplicationFiled: March 18, 2022Publication date: July 28, 2022Applicants: AGC Inc., THE UNIVERSITY OF TOKYOInventors: Takashi OKAZOE, Yuichiro ISHIBASHI, Akimitsu OKAMOTO, Kunihiko MORIHIRO, Kohsuke AIKAWA, Honoka WATANABE
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Publication number: 20180251815Abstract: The purpose of the present invention is to develop and provide a less expensive and novel oxidizing agent for 5-hydroxymethylcytosine, which can selectively oxidize 5-hydroxymethylcytosine on DNA into 5-formylcytosine while preventing the DNA structure from destabilization and suppressing side reactions, and a method for detecting a demethylation site at a high accuracy in demethylation of DNA with the use of the aforesaid oxidizing agent. Provided is an oxidizing agent for 5-hydroxymethylcytosine that comprises a nitroxyl radical molecule and a copper salt or a copper complex, and/or a nitroxyl radical molecule-copper complex.Type: ApplicationFiled: September 2, 2016Publication date: September 6, 2018Applicant: The University of TokyoInventors: Akimitsu OKAMOTO, Taku MATSUSHITA
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Patent number: 9499576Abstract: The present invention relates to a compound represented by Formulas 1, 2 or 3 as described herein, and a nucleic acid each of which contains a dye exhibiting an exciton effect, a method of producing such a nucleic acid by using the compound, and a kit for producing the nucleic acid.Type: GrantFiled: December 28, 2011Date of Patent: November 22, 2016Assignee: RIKENInventors: Akimitsu Okamoto, Shuji Ikeda
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Patent number: 8895244Abstract: Provided are a method and a kit for detecting 5-hydroxymethylcytosine in a nucleic acid. The method is a method for detecting 5-hydroxymethylcytosine in a nucleic acid, comprising the steps of: (1) oxidizing 5-hydroxymethylcytosine in a nucleic acid sample by treating the nucleic acid sample with a tungstic acid-based oxidizing agent comprising peroxotungstic acid, tungstic acid, a salt thereof, or a combination thereof with a reoxidizing agent; and (2) determining the position of the oxidized 5-hydroxymethylcytosine in the nucleic acid sample.Type: GrantFiled: April 16, 2012Date of Patent: November 25, 2014Assignee: RikenInventors: Akimitsu Okamoto, Kaori Sugizaki, Akiko Nakamura, Hiroyuki Yanagisawa, Shuji Ikeda
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Publication number: 20140045183Abstract: Provided are a method and a kit for detecting 5-hydroxymethylcytosine in a nucleic acid. The method is a method for detecting 5-hydroxymethylcytosine in a nucleic acid, comprising the steps of: (1) oxidizing 5-hydroxymethylcytosine in a nucleic acid sample by treating the nucleic acid sample with a tungstic acid-based oxidizing agent comprising peroxotungstic acid, tungstic acid, a salt thereof, or a combination thereof with a reoxidizing agent; and (2) determining the position of the oxidized 5-hydroxymethylcytosine in the nucleic acid sample.Type: ApplicationFiled: April 16, 2012Publication date: February 13, 2014Applicant: RIKENInventors: Akimitsu Okamoto, Kaori Sugizaki, Akiko Nakamura, Hiroyuki Yanagisawa, Shuji Ikeda
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Publication number: 20130289263Abstract: A compound is represented by the following formula (1), (2), or (3) (where, in the above formulae (1), (2), and (3), Z11 and Z12 independently have a fluorescent property and are an uncharged atomic group exhibiting an exciton effect).Type: ApplicationFiled: December 28, 2011Publication date: October 31, 2013Applicant: RIKENInventors: Akimitsu Okamoto, Shuji Ikeda
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Patent number: 8383792Abstract: The present invention provides, for example, a labeling substance that allows the double helix structure of a nucleic acid to be detected effectively. The present invention provides a compound having a structure derived from mononucleoside or mononucleotide, with the structure being represented by the following formula (1), (1b), or (1c), a tautomer or stereoisomer thereof, or a salt thereof. In the above formulae, B is an atomic group having a nucleobase skeleton, E is an atomic group having a deoxyribose skeleton, a ribose skeleton, or a structure derived from either one of them, or an atomic group having a peptide structure or a peptoid structure, and Z11 and Z12 each are a hydrogen atom, a protecting group, or an atomic group that exhibits fluorescence and may be identical to or different from each other.Type: GrantFiled: March 6, 2008Date of Patent: February 26, 2013Assignee: RikenInventors: Akimitsu Okamoto, Shuji Ikeda, Takeshi Kubota
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Patent number: 8354497Abstract: The present invention aims to provide a tool etc. capable of detecting a methylated region of a DNA in a short time, in a labor-saving manner and without being limited by nucleotide sequences, and further capable of quantifying the methylation. The present invention provides a peptide containing a metal finger motif and a tyrosine derivative in a helix forming part of the motif, which recognizes and binds to a methylated region of a double stranded DNA.Type: GrantFiled: October 15, 2010Date of Patent: January 15, 2013Assignee: RikenInventors: Akimitsu Okamoto, Akiko Nomura
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Publication number: 20120238038Abstract: The present invention aims to provide a tool etc. capable of detecting a methylated region of a DNA in a short time, in a labor-saving manner and without being limited by nucleotide sequences, and further capable of quantifying the methylation. The present invention provides a peptide containing a metal finger motif and a tyrosine derivative in a helix forming part of the motif, which recognizes and binds to a methylated region of a double stranded DNA.Type: ApplicationFiled: October 15, 2010Publication date: September 20, 2012Inventors: Akimitsu Okamoto, Akiko Nomura
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Patent number: 8067162Abstract: The present invention provides a primer that effectively can detect, for example, the double helix structure of a nucleic acid. The primer is a labeled nucleic acid containing at least one structure represented by the following formula (16), where B is an atomic group having a nucleobase skeleton, E is an atomic group having a deoxyribose skeleton, a ribose skeleton, or a structure derived from either one of them, or an atomic group having a peptide structure or a peptoid structure, and Z11 and Z12 are each an atomic group that exhibits fluorescence and are identical to or different from each other.Type: GrantFiled: March 10, 2008Date of Patent: November 29, 2011Assignees: Riken, Kabushiki Kaisha DnaformInventors: Yoshihide Hayashizaki, Akimitsu Okamoto, Alexander Lezhava, Yasushi Kogo
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Publication number: 20100092971Abstract: The present invention provides, for example, a labeling substance that allows the double helix structure of a nucleic acid to be detected effectively. The present invention provides a compound having a structure derived from mononucleoside or mononucleotide, with the structure being represented by the following formula (1), (1b), or (1c), a tautomer or stereoisomer thereof, or a salt thereof. In the above formulae, B is an atomic group having a nucleobase skeleton, E is an atomic group having a deoxyribose skeleton, a ribose skeleton, or a structure derived from either one of them, or an atomic group having a peptide structure or a peptoid structure, and Z11 and Z12 each are a hydrogen atom, a protecting group, or an atomic group that exhibits fluorescence and may be identical to or different from each other.Type: ApplicationFiled: March 6, 2008Publication date: April 15, 2010Applicant: RIKENInventors: Akimitsu Okamoto, Shuji Ikeda, Takeshi Kubota
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Patent number: 7563886Abstract: A method of easily releasing a useful substance bonded to oligonucleotide without impairing a target nucleic acid; and a novel base therefore. A nucleoside of nucleotide (oligonucleotide containing thereof) represented by the formula (I) (wherein each of X and Y independently represents —O—, —NH—, —N(alkyl)- or —S—; R represents a functional unit, a reporter unit or a biofunctional molecule; each of R1 and R2 independently represents a hydrogen atom, a phosphate bond group, a phosphoramidite group or a nucleotide; and n is a numeral of 1 to 10). There is further provided a method of releasing the R group moiety at base portion by the use of the oligonucleotide comprising the nucleotide.Type: GrantFiled: August 2, 2004Date of Patent: July 21, 2009Assignee: Japan Science and Technology AgencyInventors: Isao Saito, Akimitsu Okamoto, Kazuo Tanaka
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Publication number: 20080302674Abstract: A probe unit for identifying a target nucleotide region in a target nucleic acid, the unit being provided with an electrode, a probe bound to the electrode and recognizes the target nucleic acid, and a hole-transfer-inducing agent bound to the probe, wherein a nucleotide region corresponding to the target nucleotide region is located between the hole-transfer-inducing-agent-binding site and the electrode-binding end of the probe. A state of the target nucleotide region in the target nucleic acid can be identified by comparing electrochemical signal levels by energy-induced hole transfers before and after the hybridization of such a probe unit with the target nucleic acid.Type: ApplicationFiled: December 21, 2005Publication date: December 11, 2008Applicant: KYOTO UNIVERSITYInventors: Akimitsu Okamoto, Taku Kamei, Isao Saito
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Publication number: 20080227104Abstract: The present invention provides a primer that effectively can detect, for example, the double helix structure of a nucleic acid. The primer is a labeled nucleic acid containing at least one structure represented by the following formula (16), where B is an atomic group having a nucleobase skeleton, E is an atomic group having a deoxyribose skeleton, a ribose skeleton, or a structure derived from either one of them, or an atomic group having a peptide structure or a peptoid structure, and Z11 and Z12 are each an atomic group that exhibits fluorescence and are identical to or different from each other.Type: ApplicationFiled: March 10, 2008Publication date: September 18, 2008Applicants: RIKEN, KABUSHIKI KAISHA DNAFORMInventors: Yoshihide Hayashizaki, Akimitsu Okamoto, Alexander Lezhava, Yasushi Kogo
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Patent number: 7414117Abstract: A novel nucleotide derivative, in case of existing as a member of a single-stranded sequence, undergoing a change in the fluorescent signal intendity depending on the corresponding base type in the partner strand with which the single-stranded sequence is hybridized, and which is a thymin/uracil derivative (1) emitting light most intensely when a confronting base in the partner strand with which the single-stranded nucleotide sequence is hybridized is adenine; a cytosine derivative (2) emitting light most intensely when the confronting base is guanine; an adenine derivative (3) emitting light most intensely when the confronting base is cytosine; a guanine derivative (4) emitting light most intensely when the confronting base is cytosine or thymine/uracil; and an adrnine derivative (5) emitting light most intensely when the confronting base is thymine/uracil/.Type: GrantFiled: March 9, 2004Date of Patent: August 19, 2008Assignees: NGK Insulators, Ltd.Inventors: Isao Saito, Akimitsu Okamoto, Yoshio Saito, Yasuko Yoshida, Kousuke Niwa
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Patent number: 7323555Abstract: A novel nucleotide derivative, in case of existing as a member of a single-stranded sequence, undergoing a change in the fluorescent signal intensity depending on the corresponding base type in the partner strand with which the single-stranded sequence is hybridized, and which is (1) a thymine/uracil derivative emitting light most intensely when a confronting base in the partner strand with which the single-stranded nucleotide sequence is hybridized is adenine; (2) a cytosine derivative emitting light most intensely when the confronting base is guanine; (3) an adenine derivative emitting light most intensely when the confronting base is cytosine; and, (4) a guanine derivative emitting light most intensely when the confronting base is cytosine or thymine/uracil.Type: GrantFiled: December 24, 2003Date of Patent: January 29, 2008Assignees: Isao Saito, NGK Insulators, Ltd.Inventors: Isao Saito, Akimitsu Okamoto, Yasuko Yoshida
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Publication number: 20070270582Abstract: A method of easily releasing a useful substance bonded to oligonucleotide without impairing a target nucleic acid; and a novel base therefore. A nucleoside of nucleotide (oligonucleotide containing thereof) represented by the formula (I) (wherein each of X and Y independently represents —O—, —NH—, —N(alkyl)- or —S—; R represents a functional unit, a reporter unit or a biofunctional molecule; each of R1 and R2 independently represents a hydrogen atom, a phosphate bond group, a phosphoramidite group or a nucleotide; and n is a numeral of 1 to 10). There is further provided a method of releasing the R group moiety at base portion by the use of the oligonucleotide comprising the nucleotide.Type: ApplicationFiled: August 2, 2004Publication date: November 22, 2007Inventors: Isao Saito, Akimitsu Okamoto, Kazuo Tanaka
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Patent number: 7205396Abstract: A nucleic acid base for hole transportation in DNAs which does not cause oxidative decomposition; and an artificial DNA molecule which can realize effective hole transportation in DNAs while maintaining the double spiral structure of the DNAs. Provided are: a nucleic acid which contains a benzodeazaadenine derivative base represented by the general formula (I): (wherein R1, R2, R3, R4, R5, and R6 each independently represents hydrogen, amino, mono (lower alkyl) amino, di (lower alkyl) amino, hydroxy, lower alkoxy, halogeno, cyano, mercapto, lower alkylthio, or aryl; and R7 and R8 each independently represents hydrogen or a group bonded to phosphoric acid); and a polynucleotide comprising the nucleic acid.Type: GrantFiled: August 15, 2002Date of Patent: April 17, 2007Assignee: Japan Science and Technology AgencyInventors: Isao Saito, Akimitsu Okamoto, Kazuo Tanaka