Abstract: An anti-adhesion material comprising decellularized tissues and a biocompatible polymer or fibrin glue; a method for preparing an anti-adhesion material comprising complexing a biocompatible polymer or fibrin glue to decellularized tissues; an anti-adhesion material kit comprising decellularized tissues and a biocompatible polymer or fibrin glue; a substitute biomembrane comprising decellularized tissues and a biocompatible polymer or fibrin glue; a method for preparing a substitute biomembrane comprising complexing a biocompatible polymer or fibrin glue to decellularized tissues; and a substitute biomembrane kit comprising decellularized tissues and a biocompatible polymer or fibrin glue.

Abstract: An anti-adhesion material comprising decellularized tissues and a biocompatible polymer or fibrin glue; a method for preparing an anti-adhesion material comprising complexing a biocompatible polymer or fibrin glue to decellularized tissues; an anti-adhesion material kit comprising decellularized tissues and a biocompatible polymer or fibrin glue; a substitute biomembrane comprising decellularized tissues and a biocompatible polymer or fibrin glue; a method for preparing a substitute biomembrane comprising complexing a biocompatible polymer or fibrin glue to decellularized tissues; and a substitute biomembrane kit comprising decellularized tissues and a biocompatible polymer or fibrin glue.

Abstract: Provided are: a method for preparing a decellularized tissue product in which decellularized tissue can be filled with liquid while changes in the structure of support tissue constituting the decellularized tissue are inhibited; and a graft provided with a decellularized tissue product. The method for preparing a decellularized tissue product includes a reduced-pressure step for bringing an animal-derived decellularized tissue material and a liquid into contact under reduced-pressure conditions, and/or a pressurized step for bringing same into contact under pressurized conditions. The graft is provided with a decellularized tissue product prepared by the method of preparation.

Abstract: An artificial blood vessel that can be transplanted to blood vessels with a small diameter, can be adjusted to an arbitrary size of a diameter, improves in invasiveness when a graft is taken, and overcomes the problem on the provision of a graft is provided. An artificial blood vessel prepared from a decellularized tubular structure, which is prepared by processing a decellularized, sheet-like blood vessel (decellularized blood vessel sheet) into a roll structure, and a tissue adhesive, wherein a portion which is contacted with blood that flows within the artificial blood vessel consists of the tissue of the tunica intima lined with the tissue of the tunica media whereas a portion of the sheet that overlaps when the sheet is processed into a roll structure (overlap width) consists of the tissue of the tunica media and wherein a tissue adhesive is applied to the overlap width.

Abstract: A particulate decellularized tissue is provided which shows sufficiently effective tissue regeneration and does not show cytotoxicity when used as material for cell culture. In accordance with the method for preparing a particulate decellularized tissue comprising a step of applying a high hydrostatic pressure to animal-derived tissues in a medium, a particulate decellularized tissue is obtained which does not show cytotoxicity but shows the effect of cellular attraction and the effect of induction of cellular differentiation and shows a high effect of tissue regeneration.

Abstract: A hybrid gel comprising a particulate decellularized tissue (obtained by pulverizing animal-derived biological tissues that are decellularized (decellularized biological tissues)), fibrinogen and thrombin; a cell culture material comprising the hybrid gel; a method for preparing the hybrid gel; and a kit comprising a particulate decellularized tissue and a biological tissue adhesive are provided. The hybrid gel of the present invention exerts the effect to promote differentiation and gain of function of stem cells and the therapeutic effect to a variety of diseases.

Abstract: An artificial blood vessel that can be transplanted to blood vessels with a small diameter, can be adjusted to an arbitrary size of a diameter, improves in invasiveness when a graft is taken, and overcomes the problem on the provision of a graft is provided. An artificial blood vessel prepared from a decellularized tubular structure, which is prepared by processing a decellularized, sheet-like blood vessel (decellularized blood vessel sheet) into a roll structure, and a tissue adhesive, wherein a portion which is contacted with blood that flows within the artificial blood vessel consists of the tissue of the tunica intima lined with the tissue of the tunica media whereas a portion of the sheet that overlaps when the sheet is processed into a roll structure (overlap width) consists of the tissue of the tunica media and wherein a tissue adhesive is applied to the overlap width.

Abstract: Provided are: a method for preparing a decellularized tissue product in which decellularized tissue can be filled with liquid while changes in the structure of support tissue constituting the decellularized tissue are inhibited; and a graft provided with a decellularized tissue product. The method for preparing a decellularized tissue product includes a reduced-pressure step for bringing an animal-derived decellularized tissue material and a liquid into contact under reduced-pressure conditions, and/or a pressurized step for bringing same into contact under pressurized conditions. The graft is provided with a decellularized tissue product prepared by the method of preparation.

Abstract: It is intended to provide a method of preparing a decellularized soft tissue whereby swelling after an ultrahigh pressure treatment can be inhibited, etc. The above-described method of preparing a decellularized soft tissue comprises the application step wherein an ultrahigh hydrostatic pressure is applied to an isolated soft tissue in a medium to thereby disrupt cells in the soft tissue, and the removal step wherein the disrupted cells are removed. As the medium, use is made of an aqueous solution containing a water-soluble polysaccharide such as dextran.

Abstract: Methods and apparatus for controlling biological functions with mechanical vibration are provided. Stimulation is applied to cells of one of an organism, bacteria or virus by mechanical vibration. The biological function comprises biological functions relating to cell growth. The biological functions relating to cell growth may include at least one of cell cultivation, cell proliferation, cell fusion, and cell differentiation.

Abstract: A method of injecting cells into a biological tissue comprising thrusting injection needle (200) under microvibration into tissue (5) secured by suction to tissue suction means (210) and effecting injection of a cell suspension into the tissue through the needle. There is also provided an apparatus for cell injection into the biological tissue.

Abstract: A device 1a for bonding adherends T1 and T2, as a device for bonding biological tissue to biological tissue or a biological tissue bonding material, comprises: a clamping part 2a for clamping the adherends T1 and T2 between members 21a and 22a; a pressing part 3a for pressing the member 22a towards the member 21a; a pressure control part 4a for controlling the pressure by the pressing part 3a; a heating element 5a built into the member 21a; a heating control part 6a for controlling the heating by the heating element 5a; a vibration generating part 7a for generating microvibration; and a vibration control part 8a for controlling the microvibration generated by the vibration generating part 7a.

Abstract: The present invention provides: a heat-generating device which includes a heat-generating unit 5a, having a resin member 10a which generates heat upon application of vibration and a vibration unit 11a which imparts vibration to the resin member 10a, and a heat generation control unit 6a which, by controlling the vibration generated by the vibration unit 11a, controls the heat generation of the heat-generating unit 5a in such a manner that the heat-generating unit 5a has a prescribed temperature; a heat-generating method utilizing this device; and a biological tissue-bonding device 1a utilizing the device.

Abstract: Disclosed is a polymer brush composite including a substrate, a solid layer disposed on the substrate and containing at least one of a metal or a metal oxide, and a plurality of polymer chains penetrating the solid layer in such a manner that one end of each of the polymer chains is disposed on the upper side of the substrate and the other end of each the polymer chains is exposed at a surface of the solid layer on an opposite side of the solid layer to the substrate. The polymer brush composite exhibits good adhesion between an organic polymer compound and an inorganic material and has the surface the organic polymer compound and the inorganic material uniformly coexist.

Abstract: An apparatus and method for inoculating a biological substrate with cells include provision for rotating a container in which the substrate and a cell suspension are received about first and second axes of rotation. The apparatus comprises said container, first drive means for rotatably supporting the container about the first axis, and second drive means for rotatably supporting the first drive means about the second axis. The first and second axes of rotation do not lie in the same plane and extend in different directions so that the container revolves around the second axis while the container is rotating about the first axis. The inoculation method utilizes this apparatus.

Abstract: A method of efficiently removing or fixing donor cells from a native tissue of mammalian origin comprises immersing the tissue in a treating solution, and irradiating the tissue with microwave while maintaining the temperature thereof in the range between 0° C. and 40° C.

Abstract: It is intended to provide a method of preparing a decellularized soft tissue whereby swelling after an ultrahigh pressure treatment can be inhibited, etc. The above-described method of preparing a decellularized soft tissue comprises the application step wherein an ultrahigh hydrostatic pressure is applied to an isolated soft tissue in a medium to thereby disrupt cells in the soft tissue, and the removal step wherein the disrupted cells are removed. As the medium, use is made of an aqueous solution containing a water-soluble polysaccharide such as dextran.

Abstract: A reactive functional group is introduced into titanium oxide, and an active group which is capable of reacting with the reactive functional group is introduced into a polymer-based material, and the reactive functional group and the active group are reacted with each other, thereby producing a titanium oxide complex in which the polymer-based material and the titanium oxide chemically bond to each other. Further, a functional group of the polymer-based material and the titanium oxide are reacted with each other, thereby producing the titanium oxide complex without performing chemical pretreatment with respect to the titanium oxide.

Abstract: A method of injecting cells into a biological tissue comprising thrusting injection needle (200) under microvibration into tissue (5) secured by suction to tissue suction means (210) and effecting injection of a cell suspension into the tissue through the needle. There is also provided an apparatus for cell injection into the biological tissue.

Abstract: A device 1a for bonding adherends T1 and T2, as a device for bonding biological tissue to biological tissue or a biological tissue bonding material, comprises: a clamping part 2a for clamping the adherends T1 and T2 between members 21a and 22a; a pressing part 3a for pressing the member 22a towards the member 21a; a pressure control part 4a for controlling the pressure by the pressing part 3a; a heating element 5a built into the member 21a; a heating control part 6a for controlling the heating by the heating element 5a; a vibration generating part 7a for generating microvibration; and a vibration control part 8a for controlling the microvibration generated by the vibration generating part 7a.