Patents by Inventor Akira Asakura
Akira Asakura has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 7842483Abstract: The present invention is directed to a novel cytochrome c oxidase complex, genetic materials useful for the preparation of the said complex, such as recombinant polypeptides involved in cytochrome c oxidase complex, recombinant DNA fragments, expression vectors, recombinant organisms and the like. Those novel cytochrome c oxidase complex and genetic materials may be originated from a microorganism having the identifying characteristics of Gluconobacter oxydans DSM 4025. The present invention also provides a method for the preparation of the said novel recombinant cytochrome c oxidase complex and a process for the production of 2-keto-L-gulonic acid (2KGA).Type: GrantFiled: January 26, 2005Date of Patent: November 30, 2010Assignee: DSM Nutritional Products, Inc.Inventors: Akira Asakura, Tatsuo Hoshino, Masako Shinjoh
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Publication number: 20050123989Abstract: The present invention is directed to a novel cytochrome c oxidase complex, genetic materials useful for the preparation of the said complex, such as recombinant polypeptides involved in cytochrome c oxidase complex, recombinant DNA fragments, expression vectors, recombinant organisms and the like. Those novel cytochrome c oxidase complex and genetic materials may be originated from a microorganism having the identifying characteristics of Gluconobacter oxydans DSM 4025. The present invention also provides a method for the preparation of the said novel recombinant cytochrome c oxidase complex and a process for the production of 2-keto-L-gulonic acid (2KGA).Type: ApplicationFiled: January 26, 2005Publication date: June 9, 2005Inventors: Akira Asakura, Tatsuo Hoshino, Masako Shinjoh
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Publication number: 20050090645Abstract: The present invention is directed to a recombinant enzymes having alcohol and aldehyde dehydrogenase activity which comprises one or more recombinant polypeptides selected from the group consisting of polypeptides which are identified by SEQ ID NO 5, SEQ ID NO 6, SEQ ID NO 7, SEQ ID NO 8 and chimeric recombinant polypeptides that are a chimeric combination of at least two of the following amino acid sequences identified by SEQ ID NO 5, SEQ ID NO 6, SEQ ID NO 7, SEQ ID NO 8 and functional derivatives of the polypeptides identified above which contain addition, insertion, deletion and/or substitution of one or more amino acid residues, wherein said enzymatic polypeptides have said alcohol and aldehyde dehydrogenase activity. DNA molecules encoding the recombinant polypeptides, vectors comprising such DNA molecules, host cells transformed by such vectors and processes for the production of such recombinant enzymes are provided.Type: ApplicationFiled: March 17, 2004Publication date: April 28, 2005Inventors: Akira Asakura, Tatsuo Hoshino, Setsuko Ojima, Masako Shinjoh, Noribumi Tomiyama
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Patent number: 6869785Abstract: The present invention is directed to a novel cytochrome c oxidase complex, genetic materials useful for the preparation of the said complex, such as recombinant polypeptides involved in cytochrome c oxidase complex, recombinant DNA fragments, expression vectors, recombinant organisms and the like. Those novel cytochrome c oxidase complex and genetic materials may be originated from a microorganism having the identifying characteristics of Gluconobacter oxydans DSM 4025. The present invention also provides a method for the preparation of the said novel recombinant cytochrome c oxidase complex and a process for the production of 2-keto-L-gulonic acid (2KGA).Type: GrantFiled: November 14, 2000Date of Patent: March 22, 2005Assignee: DSM Nutritional Products, Inc.Inventors: Akira Asakura, Tatsuo Hoshino, Masako Shinjoh
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Patent number: 6777212Abstract: A process for producing L-ascorbic acid or D-erythorbic acid, or in each case its sodium, potassium or calcium salt, from 2-keto-L-gulonic acid or 2-keto-D-gluconic acid, or in each case its sodium, potassium or calcium salt, that involves incubating 2-keto-L-gulonic acid or 2-keto-D-gluconic acid, each as the free acid or as its sodium, potassium or calcium salt, and the cells of a thermoacidophilic microorganism at temperatures from about 30° C. to about 100° C., and at a pH from about 1 to about 6, in a solution, to form L-ascorbic acid or D-erythorbic acid or an appropriate salt thereof, and isolating said product from the solution.Type: GrantFiled: August 23, 2001Date of Patent: August 17, 2004Assignee: Roche Vitamins, Inc.Inventors: Akira Asakura, Tatsuo Hoshino, Masako Shinjoh
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Publication number: 20040137009Abstract: A novel human IFN-&agr; subtype and its derivative having an unprecedentedly high specific activity, DNA encoding these proteins, an expression vector having said DNA, a transformant transformed with said expression vector, a method of producing the above human IFN-&agr; and its derivative, and medical uses of the above human IFN-&agr; and its derivative.Type: ApplicationFiled: January 15, 2004Publication date: July 15, 2004Applicant: SUMITOMO PHARMACEUTICALS COMPANY LIMITEDInventors: Shin-ichi Kojima, Akira Asakura, Tetsuaki Futatsugi, Yuko Ota, Yuki Fukuda, Shinsuke Sagara
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Patent number: 6730503Abstract: The present invention is directed to a recombinant enzymes having alcohol and aldehyde dehydrogenase activity which comprises one or mom recombinant polypeptides selected from the group consisting of polypeptides which are identified by SEQ ID NO 5, SEQ ID NO 6, SEQ ID NO 7, SEQ ID NO 8 and chimeric recombinant polypeptides that are a chimeric combination of at least two of the following amino acid sequences identified by SEQ ID NO 5, SEQ ID NO 6, SEQ ID NO 7, SEQ ID NO 8 and functional derivatives of the polypeptides identified above which contain addition, insertion, deletion and/or substitution of one or more amino acid residues, wherein said enzymatic polypeptides have said alcohol and aldehyde dehydrogenase activity. DNA molecules encoding the recombinant polypeptides, vectors comprising such DNA molecules, host cells transformed by such vectors and processes for the production of such recombinant enzymes are provided.Type: GrantFiled: December 22, 1999Date of Patent: May 4, 2004Assignee: Roche Vitamins Inc.Inventors: Akira Asakura, Tatsuo Hoshino, Setsuko Ojima, Masako Shinjoh, Noribumi Tomiyama
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Publication number: 20040063166Abstract: The present invention provides processes for making biotin from desthiobiotin by either contacting desthiobiotin with an enzyme reaction mixture containing bioB gene product and nifU gene product and/or nifS gene product and isolating the biotin or cultivating a microorganism transformed with DNA encoding the bioB gene product, nifU gene product and nifS gene product and isolating the biotin.Type: ApplicationFiled: May 15, 2003Publication date: April 1, 2004Applicant: ROCHE VITAMINS, INC.Inventors: Tatsuo Hoshino, Akira Asakura, Tatsuya Kiyasu, Yoshie Nagahashi
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Patent number: 6703225Abstract: A novel human IFN-&agr; subtype and its derivative having an unprecedentedly high specific activity, DNA encoding these proteins, an expression vector having said DNA, a transformant transformed with said expression vector, a method of producing the above human IFN-&agr; and its derivative, and medical uses of the above human IFN-&agr; and its derivative.Type: GrantFiled: October 24, 2001Date of Patent: March 9, 2004Assignee: Sumitomo Pharmaceuticals Company, LimitedInventors: Shin-ichi Kojima, Akira Asakura, Tetsuaki Futatsugi, Yuko Ota, Yuki Fukuda, Shinsuke Sagara
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Publication number: 20030105160Abstract: A process for producing L-ascorbic acid or D-erythorbic acid, or in each case its sodium, potassium or calcium salt, from 2-keto-L-gulonic acid or 2-keto-D-gluconic acid, or in each case its sodium, potassium or calcium salt, that involves incubating 2-keto-L-gulonic acid or 2-keto-D-gluconic acid, each as the free acid or as its sodium, potassium or calcium salt, and the cells of a thermoacidophilic microorganism at temperatures from about 30° C. to about 100° C., and at a pH from about 1 to about 6, in a solution, to form L-ascorbic acid or D-erythorbic acid or an appropriate salt thereof, and isolating said product from the solution.Type: ApplicationFiled: August 23, 2001Publication date: June 5, 2003Applicant: ROCHE VITAMINS INC.Inventors: Akira Asakura, Tatsuo Hoshino, Masako Shinjoh
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Publication number: 20030096382Abstract: The present invention provides process for making biotin from desthiobiotin either contacting desthiobiotin with an enzyme reaction mixture containing bioB gene product and nifU gene product and/or nifS gene product and isolating the biotin or cultivating a microorganism transformed with DNA encoding the bioB gene product, nifU gene product and nifS gene product and isolating the biotin.Type: ApplicationFiled: March 22, 2001Publication date: May 22, 2003Applicant: ROCHE VITAMINS INC.Inventors: Tatsuo Hoshino, Akira Asakura, Tatsuya Kiyasu, Yoshie Nagahashi
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Patent number: 6407203Abstract: This invention is directed to cytochrome c551 polypeptides and polynucleotides encoding the polypeptides.Type: GrantFiled: December 29, 1999Date of Patent: June 18, 2002Assignee: Roche Vitamins, Inc.Inventors: Akira Asakura, Tatsuo Hoshino, Masako Shinjoh, Noribumi Tomiyama
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Patent number: 6361978Abstract: The present invention provides processes for making biotin from desthiobiotin by either contacting desthiobiotin with an enzyme reaction mixture containing bioB gene product and nifU gene product and/or nifS gene product and isolating the biotin or cultivating a microorganism transformed with DNA encoding the bioB gene product, nifU gene product and nifS gene product and isolating the biotin.Type: GrantFiled: April 24, 1997Date of Patent: March 26, 2002Assignee: Roche Vitamins, Inc.Inventors: Tatsuo Hoshino, Akira Asakura, Tatsuya Kiyasu, Yoshie Nagahashi
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Patent number: 6146860Abstract: A process for producing L-ascorbic acid (vitamin C) from 2-keto-L-gulonic acid or D-erythorbic acid from 2-keto-D-gluconic acid by contacting 2-keto-L-gulonic acid or 2-keto-D-gluconic acid, respectively, in solution with a lactonase, particularly one belonging to the enzyme class EC 3.1.1.x, according to the classification of Enzyme Nomenclature. The solvent for this reaction can be water, an aqueous alcohol, a non-alcoholic organic solvent or a mixture of an aqueous alcohol and a non-alcoholic organic solvent. The contacting is generally performed in a temperature range of 0.degree. C. to 120.degree. C. and a pH range of 1.5 to 12. In each case the starting material can be in the form of the free acid, the sodium salt, or the calcium salt. The so-produced vitamin C has very well known uses, and the alternatively produced D-erythorbic acid is useful as an antioxidant for food additives.Type: GrantFiled: January 18, 2000Date of Patent: November 14, 2000Assignee: Roche Vitamins Inc.Inventors: Akira Asakura, Tatsuo Hoshino, Tatsuya Kiyasu, Masako Shinjoh
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Patent number: 6037147Abstract: This invention is directed to cytochrome c.sub.551 polypeptides and polynucleotides encoding the polypeptides.Type: GrantFiled: March 31, 1998Date of Patent: March 14, 2000Assignee: Roche Vitamins Inc.Inventors: Akira Asakura, Tatsuo Hoshino, Masako Shinjoh, Noribumi Tomiyama
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Patent number: 5932463Abstract: The present invention relates to a novel alcohol/aldehyde dehydrogenase (hereinafter referred to as AADH), a process for producing the same and a process for producing aldehydes, carboxylic acids and ketones, especially 2-keto-L-gulonic acid (hereinafter referred to as 2-KGA) utilizing said enzyme.Type: GrantFiled: June 20, 1997Date of Patent: August 3, 1999Assignee: Roche Vitamins Inc.Inventors: Akira Asakura, Tatsuo Hoshino
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Patent number: 5916785Abstract: The present invention relates to a novel alcohol/aldehyde dehydro-genase (hereinafter referred to as AADH), a process for producing the same and a process for producing aldehydes, carboxylic acids and ketones, especially 2-keto-L-gulonic acid (hereinafter referred to as 2-KGA) utilizing said enzyme.Type: GrantFiled: June 20, 1997Date of Patent: June 29, 1999Assignee: Roche Vitamins Inc.Inventors: Akira Asakura, Tatsuo Hoshino
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Patent number: 5437989Abstract: A homogeneous alcohol/aldehyde dehydrogenase isolated from Gluconobacter oxydans DSM 4025 FERM BP-3812 is disclosed. The enzyme is capable in the presence of an electron acceptor, of catalyzing the conversion of L-sorbose to 2-keto-L-gulonic acid via L-sorbosone. The enzyme has a molecular weight of 130,000-140,000 daltons as determined by gel filtration column chromatography, a pyrroloquinoline quinone prosthetic group, an optimum pH for enzyme activity of 7.0 to 9.0, an optimum temperature for enzyme activity from about 20.degree. C. to 40.degree. C. and an isoelectric point of about 4.4. Also disclosed is a process for producing the enzyme and a process for producing aldehydes, carboxylic acids and ketones, especially 2-keto-L-gulonic acid utilizing the enzyme.Type: GrantFiled: December 3, 1993Date of Patent: August 1, 1995Assignee: Hoffmann-La Roche Inc.Inventors: Akira Asakura, Tatsuo Hoshino
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Patent number: 4405408Abstract: A cellulose treating agent obtained by dispersing an alkyl ketene dimer in water in the presence of a dispersing agent comprising a reaction product of a polyalkylenepolyamine and a mixture of carboxylic acids containing at least 20% by weight of aliphatic monohydroxymonocarboxylic acids having 9 to 32 carbon atoms or a product obtained by reacting epihalohydrin with said reaction product. This cellulose treating agent existing as a very stable cationic aqueous dispersion of the alkyl ketene dimer is highly effective to impart a water repellency to cellulose fibers and can be used as a well-improved reactive sizing agent for papermaking.Type: GrantFiled: January 20, 1982Date of Patent: September 20, 1983Assignee: Seiko Kagaku Kogyo Co., Ltd.Inventors: Shigehiko Yoshioka, Hideto Yamada, Akira Asakura