Abstract: According to the present invention, a device for measuring an object to be measured in a sample which comprises a support, sample addition site (S) and a detection site (Q), said sample addition site and said detection site being on the support, said support allowing the object to be measured to move by the capillary flow of a developing liquid, said detection site having a piezoelectric vibrator sandwiched between two electrodes, said piezoelectric vibrator having a trapper A, (c1) immobilized thereon, or an analogue of the object to be measured (c1?) immobilized thereon, and said support further comprising a binder retaining site(BR) where a binder (b1) is retained therein so that it is movable by the capillary flow of the developing liquid. Furthermore, the present invention provides a method for quantitatively determining an object to be measured in a sample using the device.

Abstract: The present invention relates to a method of detecting a substance to be detected in a sample characterized by mixing a sample containing the substance to be detected, in a solution, with carriers which have a shape such that, when suspended in a solution and flowed through a hollow tube for detecting signals formed in a signal detecting means capable of detecting a signal A and a signal B, two or more of the carriers do not move in an overlapping manner to the cross section of the hollow tube, to which a substance capable of binding to the substance to be detected (hereinafter referred simply to as “binding element”) is bound through or not through a spacer, and which have an identifiable signal A; binding the substance to be detected to said binding element thereby causing a signal B to be formed on the carriers; flowing a suspension of said carriers through the hollow tube by a solution supply means connected to the hollow tube; detecting the signal A and the signal B by the signal detecting means; and rel

Abstract: The present invention relates to a method for conveniently quantitating TGs contained in various lipoproteins. A method for quantitating trigyceride (TG) in a particular lipoprotein which comprises eliminating free glycerol from a sample containing free glycerol and TG in the particular lipoprotein, then allowing the resulting sample to react with lipoprotein lipase and an enzyme system which generates hydrogen peroxide from free glycerol, and quantitating the generated hydrogen peroxide, is provided.

Abstract: The present invention relates to a method for conveniently quantitating TGs contained in various lipoproteins. A method for quantitating trigyceride (TG) in a particular lipoprotein which comprises eliminating free glycerol from a sample containing free glycerol and TG in the particular lipoprotein, then allowing the resulting sample to react with lipoprotein lipase and an enzyme system which generates hydrogen peroxide from free glycerol, and quantitating the generated hydrogen peroxide, is provided.

Abstract: The present invention provides a simple method for the determination of a specific component, e.g. 1,5-anhydroglucitol (1,5-AG) in a sample containing glucose, and a reagent and a reagent kit useful in the method. In one embodiment, a method for the determination of 1,5-AG is provided which comprises contacting the sample with an enzyme system which converts glucose into fructose-1,6-diphosphate and converts 1,5-AG into 1,5-AG-6-phosphate to form 1,5-AG-6-phosphate, dehydrogenating 1,5-AG-6-phosphate in the sample by the action of 1,5-AG-6-phosphate dehydrogenase in the presence of an oxidized coenzyme, and measuring the amount of the reduced coenzyme formed by the dehydrogenation reaction. A reagent and a reagent kit useful in this method are also provided.

Abstract: The present invention provides a method for determining 1,5-anhydroglucitol (1,5-AG) in a sample containing 1,5-AG, maltose and glucose, which comprises: converting maltose in the sample into glucose using an enzyme system capable of converting maltose into glucose; converting glucose into a compound which is not phosphorylated by 1,5-anhydroglucitol 6-phosphorylating enzyme system (AG-6P-ES) or dehydrogenated by the action of 1,5-anhydroglucitol-6-phosphate dehydrogenase (AG-6PDH), using an enzyme system capable of converting glucose into said compound; converting 1,5-AG into 1,5-anhydroglucitol-6-phosphate (1,5-AG-6P) using the AG-6P-ES; dehydrogenating the formed 1,5-AG-6P with AG-6PDH in the presence of an oxidized coenzyme; and determining the component formed or reduced by the dehydrogenation reaction. A reagent and a reagent kit useful in this method are also provided.

Abstract: The present invention provides a simple method for the determination of a specific component, e.g. 1,5-anhydroglucitol (1,5-AG) in a sample containing glucose, and a reagent and a reagent kit useful in the method. In one embodiment, a method for the determination of 1,5-AG is provided which comprises contacting the sample with an enzyme system which converts glucose into fructose-1,6-diphosphate and converts 1,5-AG into 1,5-AG-6-phosphate to form 1,5-AG-6-phosphate, dehydrogenating 1, 5-AG-6-phosphate in the sample by the action of 1,5-AG-6-phosphate dehydrogenase in the presence of an oxidized coenzyme, and measuring the amount of the reduced coenzyme formed by the dehydrogenation reaction. A reagent and a reagent kit useful in this method are also provided.

Abstract: The present invention provides a simple method for the determination of a specific component, e.g. 1,5-anhydroglucitol (1,5-AG) in a sample containing glucose, and a reagent and a reagent kit useful in the method. In one embodiment, a method for the determination of 1,5-AG is provided which comprises contacting the sample with an enzyme system which converts glucose into fructose-1,6-diphosphate and converts 1,5-AG into 1,5-AG-6-phosphate to form 1,5-AG-6-phosphate, dehydrogenating 1,5-AG-6-phosphate in the sample by the action of 1,5-AG-6-phosphate dehydrogenase in the presence of an oxidized coenzyme, and measuring the amount of the reduced coenzyme formed by the dehydrogenation reaction. A reagent and a reagent kit useful in this method are also provided.

Abstract: A reagent, kit and method for the quantative determination of ascorbic acid in a sample uses ascorbate oxidase which catalyzes the reaction of reduced ascorbic acid to oxidized ascorbic acid and hydrogen peroxide. Ascorbic acid in the sample is reacted with oxygen in the presence of the ascorbate oxidase, chromogen and peroxidase. Absorbance is determined and compared with a known calibration curve.

Abstract: Provided is a method of determining the amount of cholesterol in high-density lipoprotein (HDL), which comprises reacting an HDL-containing sample with cholesterol esterase and cholesterol oxidase or cholesterol dehydrogenase in the presence of a reagent for aggregating lipoproteins- except HDL, and determining the amount of hydrogen peroxide or reductive co-enzyme formed therein.

Abstract: Disclosed are a method for quantitative determination of bilirubin and a reagent for the method, which are useful in clinical examinations. The method comprises subjecting the bilirubin in a sample to coexist with ascorbate oxidase and a reaction promoter in aqueous medium to thereby oxidize the bilirubin, measuring the change in absorbance of the aqueous medium and comparing the change in absorbance with a calibration curve. The reagent comprises ascorbate oxidase and a reaction promoter.

Abstract: The present invention relates to a method for quantitatively determining a peroxidation-active substance, hydrogen peroxide or a coumarin derivative represented by formula (I) or (II): ##STR1## wherein R.sup.1, R.sup.2, R.sup.3, R.sup.4, R.sup.5 and R.sup.6 are the same or different and each represents hydrogen, substituted or unsubstituted lower alkyl, substituted or unsubstituted aralkyl, substituted or unsubstituted aryl or halogen; the method comprising reacting the hydrogen peroxide with the coumarin derivative represented by formula (I) or (II) or its salt in the presence of the peroxidation-active substance, and determining the amount of the light emission or the light intensity from the reaction solution.

Abstract: The present invention relates to a method for quantitatively determining ionized calcium in a sample by using a phospholipase, which comprises carrying out an enzyme reaction with the phospholipase in a buffer comprising a nitrogen heterocycle-binding sulfonic acid having a pK ranging 6.6 to 7.6 or a salt thereof.According to the method of the invention, the amount of ionized calcium in a sample, such as serum, can be accurately determined.

Abstract: The present invention relates to a method for the determination of cholesterol in low-density lipoprotein (LDL) in a sample containing LDL, which comprises eliminating cholesterol in high-density lipoprotein in the sample, subjecting the sample to a reaction utilizing the action of a cholesterol ester-hydrolyzing enzyme and the action of a cholesterol-oxidizing enzyme or of cholesterol oxidoreductase, and determining the amount of hydrogen peroxide or a reduced type coenzyme generated by the reaction.

Abstract: Disclosed is a chemiluminescent method of quantitatively determining one of peroxide, a peroxidation-active substance or a pyrazolopyridopyridazine derivative represented by the formula (I): ##STR1## wherein R.sup.1 represents hydrogen, lower alkyl, optionally substituted aryl, optionally substituted heterocyclic group, substituted sulfonyl, substituted sulfinyl, substituted thio etc.; R.sup.2 represents hydrogen, lower alkyl, optionally substituted aryl, etc., or a salt thereof; characterized by subjecting an unknown amount of one substance selected from the peroxide, peroxidation-active substance and pyrazolopyridopyridazine derivative to react with a known amount of the remaining two substances.

Abstract: Disclosed is a method for stably storing a liquid diagnostic reagent, comprising air-hermetically keeping the liquid diagnostic reagent in a closed container in the presence of a disoxidant therein. Preferably, at least one of the liquid diagnostic reagent and the disoxidant is covered with a separating container made of a material pervious to oxygen but not to solutions. The liquid diagnostic reagent may comprise an enzyme or an indicator.

Abstract: The present invention relates to a method for quantitatively determining sodium ions in a sample using .beta.-galactosidase in an aqueous medium, wherein a .beta.-galactosidase reaction is carried out in the presence of a cation which competes with the sodium ion.The method of the present invention is good in accurate determination and reproducibility and enables the simple and quick quantitative determination of sodium ions.

Abstract: Provided is a method of determining the amount of cholesterol in high-density lipoprotein (HDL), which comprises reacting an HDL-containing sample with cholesterol esterase and cholesterol oxidase or cholesterol dehydrogenase in the presence of a reagent for aggregating lipoproteins except HDL, and determining the amount of hydrogen peroxide or reductive co-enzyme formed therein.

Abstract: The present invention provides a new analytical method that an antigen, an antibody or a nucleic acid can be assayed at a high sensitivity in the immunoassay.The present invention provides a method for assaying a substance which comprises the steps of (a) reacting an antigen, an antibody or a nucleic acid which is the substance to be measured with an antibody, an antigen or a nucleic acid to which a polyacridinium compound is bound and which can be bound to the substance to be measured, (b) carrying out a luminescence treatment, and (c) measuring the luminescent intensity of the reaction solution.

Abstract: A method for quantitating accurately 1,5-anhydroglucitol, fructose or amylase in a sample that contains galactose using an enzymatic determination involves a pretreatment which removes galactose from the sample by an enzyme reaction in which galactose is a substrate.