Abstract: The present invention relates to a process for the manufacturing of calcium; {4-[(5, 6-diphenylpyrazin-2-yl)(propan-2-yl)amino]butoxy}acetate, or a pharmaceutically acceptable hydrate or solvate thereof. Moreover, it relates to calcium; {4-[(5,6-diphenylpyrazin-2-yl)(propan-2-yl)amino]butoxy}acetate with high purity, as well as to crystalline forms of calcium; {4-[(5,6-diphenylpyrazin-2-yl)(propan-2-yl)amino]butoxy}acetate and hydrates and solvates thereof. Furthermore, the invention relates to the use of calcium; {4-[(5, 6-diphenylpyrazin-2-yl)(propan-2-yl)amino]butoxy}acetate for the treatment or prevention of e.g. pulmonary arterial hypertension (PAH) or chronic thromboembolic pulmonary hypertension (CTEPH).

Abstract: Starting from a herbaceous cutting, which may be derived from in vitro culture, of stock and of a herbaceous scion, the process consists of inserting the scion (12') having a single bud, into the stock cutting (12), by bevelling the lower end 15 of the scion 12' from two sides 17, 18, by slitting the upper end of the stock cutting in its middle, and by subsequently assembling the two parts 12, 12' by joining 20, of transferring the base of the abovementioned grafted unit into a development medium 21, of moistening the development medium 21 with a nutrient solution and of subsequently placing this unit in a container with a water-saturated atmosphere and at a temperature above 20.degree. C., while suppressing the bud 22 of the stock and growing the saplings in the greenhouse or under a polyethylene tunnel until the desired stage of growth.

Abstract: The grafting machine comprises two assemblies, namely a top assembly 4 and a bottom assembly 3, mounted on two guide columns 2 joined at the bottom to a base 21 and at the top to a brace 5; the top assembly 4 has a scraper blade 12 associated with a cutter blade 14 for the stock, which is inserted into a centering V 8 situated at the top of the bottom assembly 3, which in its bottom part has two cutter blades 9 which, on the operation of the machine, are applied against a scion support 6 in which a scion is received in a central groove 16, the scion support being disposed on the base 21 together with a locking stop 7 facing it, one on each side of the two guide columns 2.