Abstract: The subject of the invention is a chromatography device comprising: (i) a unit column intended to receive a chromatographic bed, (ii) a removable reservoir for a bed slurry, and (iii) a base comprising a cylinder adapted to a piston, said piston sliding in the removable reservoir and the column to compress the slurry of solid particles into a chromatographic bed; the column, reservoir and base being connected in series in this order, the piston acting to compress the slurry of solid particles into a chromatographic bed in the column. A further subject of the invention is a column for use in the device, a method to pack a chromatographic bed and a separation method using the device or the column.