Patents by Inventor Alain Mir

Alain Mir has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20230257698
    Abstract: Provided herein are compositions comprising populations of cells obtained from a pregnant subject comprising at least one of a Hofbauer cell, a cytotrophoblast, a syncytiotrophoblast, an extravillous trophoblast, a villous core stroma, or a fetal vascular cell; a stabilization buffer, wherein the stabilization buffer comprises a preservative; and a cellulosic collection device, wherein at least a subset of the population of cells are intact and inviable and methods of making and using such compositions.
    Type: Application
    Filed: July 6, 2021
    Publication date: August 17, 2023
    Inventors: Stephen GIRE, Ridhi TARIYAL, Alain MIR
  • Publication number: 20230054869
    Abstract: The present disclosure provides methods, compositions, and systems employing blocked primers. Aspects of the disclosure include providing a blocked primer reaction mixture that includes a blocked primer and a template nucleic acid component from a single cell; unblocking the blocked primer to produce an active primer reaction mixture and subjecting the activated primer reaction mixture to primer extension conditions, such as nucleic acid implication conditions.
    Type: Application
    Filed: May 26, 2022
    Publication date: February 23, 2023
    Inventors: Thomas D. Schaal, Jude Menlo Dunne, Maithreyan Srinivasan, Alain Mir
  • Patent number: 11371087
    Abstract: The present disclosure provides methods, compositions, and systems employing blocked primers. Aspects of the disclosure include providing a blocked primer reaction mixture that includes a blocked primer and a template nucleic acid component from a single cell; unblocking the blocked primer to produce an active primer reaction mixture and subjecting the activated primer reaction mixture to primer extension conditions, such as nucleic acid amplification conditions.
    Type: Grant
    Filed: June 8, 2017
    Date of Patent: June 28, 2022
    Assignee: Takara Bio USA, Inc.
    Inventors: Thomas D. Schaal, Jude Menlo Dunne, Maithreyan Srinivasan, Alain Mir
  • Publication number: 20220178916
    Abstract: The present invention is directed to methods for identifying the presence of one or more target nucleotide sequences in a sample that involve a nuclease-ligation reaction. In some embodiments, the ligation products formed in the nuclease-ligation process of the present invention are subsequently amplified using a polymerase chain reaction.
    Type: Application
    Filed: December 16, 2021
    Publication date: June 9, 2022
    Inventors: Francis BARANY, Eugene SPIER, Alain MIR
  • Patent number: 11209424
    Abstract: The present invention is directed to methods for identifying the presence of one or more target nucleotide sequences in a sample that involve a ligation and/or polymerase reaction. In some embodiments, the ligation products formed in the ligation process of the present invention are subsequently amplified using a polymerase chain reaction. The ligated product sequences or extension products thereof are detected, and the presence of one or more target nucleotide sequences in the sample is identified based on the detection.
    Type: Grant
    Filed: May 24, 2019
    Date of Patent: December 28, 2021
    Assignee: CORNELL UNIVERSITY
    Inventors: Francis Barany, Eugene Spier, Alain Mir
  • Publication number: 20210324473
    Abstract: Provided herein are methods for altering a health state of a subject by administering a wellness intervention to subjects found to have quantitative measures of microbial genera in the subject's microbiome that are associated with undesirable health states. Undesirable health states can be inferred by executing models that predict health states based on the quantitative measures, such as relative amounts of selected microbial genera to all microbes. Models are created by statistical methods that analyze datasets that include, for each of a plurality of subjects, verified health states and quantitative measures of each of a plurality of microbes classified at designated taxonomic levels, e.g., genus level.
    Type: Application
    Filed: March 17, 2021
    Publication date: October 21, 2021
    Inventors: Alain MIR, Harris SHAPIRO, Ronald Avignone, Mark Kelleher
  • Patent number: 10982283
    Abstract: Provided herein are methods for altering a health state of a subject by administering a wellness intervention to subjects found to have quantitative measures of microbial genera in the subject's microbiome that are associated with undesirable health states. Undesirable health states can be inferred by executing models that predict health states based on the quantitative measures, such as relative amounts of selected microbial genera to all microbes. Models are created by statistical methods that analyze datasets that include, for each of a plurality of subjects, verified health states and quantitative measures of each of a plurality of microbes classified at designated taxonomic levels, e.g., genus level.
    Type: Grant
    Filed: April 19, 2020
    Date of Patent: April 20, 2021
    Assignee: Biome Health, Inc.
    Inventors: Alain Mir, Harris Shapiro, Ronald Avignone, Mark Kelleher
  • Publication number: 20200370095
    Abstract: The invention pertains to methods for assessing a section of a biological sample, e.g., for determining the location of an analyte in the section. In certain embodiments, the methods comprise: a) contacting the biological sample section with an oligonucleotide indexed surface comprising an addressable array of capture oligonucleotides; b) probing the oligonucleotide indexed surface contacted with the biological sample section with an analyte-specific binding member that specifically binds to the analyte, wherein the analyte-specific binding member comprises a detector oligonucleotide; c)linking the detector oligonucleotide to a barcoded capture oligonucleotide proximal thereto to produce a linked product nucleic acid, e.g., a ligated nucleic acid or an extension product nucleic acid; and d) sequencing the linked product nucleic acid to assess the biological sample for the analyte. Kits for carrying out the methods of the invention are also provided.
    Type: Application
    Filed: May 22, 2020
    Publication date: November 26, 2020
    Inventors: Andrew Alan Farmer, George G. Jokhadze, Alain Mir
  • Publication number: 20200270699
    Abstract: Provided herein are methods for altering a health state of a subject by administering a wellness intervention to subjects found to have quantitative measures of microbial genera in the subject's microbiome that are associated with undesirable health states. Undesirable health states can be inferred by executing models that predict health states based on the quantitative measures, such as relative amounts of selected microbial genera to all microbes. Models are created by statistical methods that analyze datasets that include, for each of a plurality of subjects, verified health states and quantitative measures of each of a plurality of microbes classified at designated taxonomic levels, e.g., genus level.
    Type: Application
    Filed: April 19, 2020
    Publication date: August 27, 2020
    Inventors: Alain MIR, Harris SHAPIRO, Ronald Avignone, Mark Kelleher
  • Publication number: 20190346430
    Abstract: The present invention is directed to methods for identifying the presence of one or more target nucleotide sequences in a sample that involve a nuclease-ligation reaction. In some embodiments, the ligation products formed in the nuclease-ligation process of the present invention are subsequently amplified using a polymerase chain reaction.
    Type: Application
    Filed: May 24, 2019
    Publication date: November 14, 2019
    Inventors: Francis BARANY, Eugene SPIER, Alain MIR
  • Patent number: 10338062
    Abstract: The present invention is directed to methods for identifying the presence of one or more target nucleotide sequences in a sample that involve a nuclease-ligation reaction. In some embodiments, the ligation products formed in the nuclease-ligation process of the present invention are subsequently amplified using a polymerase chain reaction. The ligated product sequences or extension products thereof are detected, and the presence of one or more target nucleotide sequences in the sample is identified based on the detection.
    Type: Grant
    Filed: February 6, 2017
    Date of Patent: July 2, 2019
    Assignee: Cornell University
    Inventors: Francis Barany, Eugene Spier, Alain Mir
  • Publication number: 20190112648
    Abstract: The present disclosure provides methods, compositions, and systems employing blocked primers. Aspects of the disclosure include providing a blocked primer reaction mixture that includes a blocked primer and a template nucleic acid component from a single cell; unblocking the blocked primer to produce an active primer reaction mixture and subjecting the activated primer reaction mixture to primer extension conditions, such as nucleic acid amplification conditions.
    Type: Application
    Filed: June 8, 2017
    Publication date: April 18, 2019
    Inventors: Thomas D. Schaal, Jude Dunne, Maithreyan Srinivasan, Alain Mir
  • Patent number: 9909179
    Abstract: The present invention provides methods for analysis of genomic DNA and/or RNA from small samples or even single cells. Methods for analyzing genomic DNA can entail whole genome amplification (WGA), followed by preamplification and amplification of selected target nucleic acids. Methods for analyzing RNA can entail reverse transcription of the desired RNA, followed by preamplification and amplification of selected target nucleic acids.
    Type: Grant
    Filed: December 21, 2015
    Date of Patent: March 6, 2018
    Assignee: FLUIDIGM CORPORATION
    Inventors: Amy Hamilton, Min Lin, Alain Mir, Martin Pieprzyk
  • Publication number: 20170212103
    Abstract: The present invention is directed to methods for identifying the presence of one or more target nucleotide sequences in a sample that involve a nuclease-ligation reaction. In some embodiments, the ligation products formed in the nuclease-ligation process of the present invention are subsequently amplified using a polymerase chain reaction.
    Type: Application
    Filed: February 6, 2017
    Publication date: July 27, 2017
    Inventors: Francis BARANY, Eugene SPIER, Alain MIR
  • Patent number: 9598728
    Abstract: The present invention is directed to methods for identifying the presence of one or more target nucleotide sequences in a sample that involve a nuclease-ligation reaction. In some embodiments, the ligation products formed in the nuclease-ligation process of the present invention are subsequently amplified using a polymerase chain reaction. The ligated product sequences or extension products thereof are detected, and the presence of one or more target nucleotide sequences in the sample is identified based on the detection.
    Type: Grant
    Filed: February 14, 2013
    Date of Patent: March 21, 2017
    Assignee: Cornell University
    Inventors: Francis Barany, Eugene Spier, Alain Mir
  • Publication number: 20160340728
    Abstract: The present invention provides methods for analysis of genomic DNA and/or RNA from small samples or even single cells. Methods for analyzing genomic DNA can entail whole genome amplification (WGA), followed by preamplification and amplification of selected target nucleic acids. Methods for analyzing RNA can entail reverse transcription of the desired RNA, followed by preamplification and amplification of selected target nucleic acids.
    Type: Application
    Filed: December 21, 2015
    Publication date: November 24, 2016
    Inventors: Amy Hamilton, Min Lin, Alain Mir, Martin Pieprzyk
  • Patent number: 9249459
    Abstract: The present invention provides methods for analysis of genomic DNA and/or RNA from small samples or even single cells. Methods for analyzing genomic DNA can entail whole genome amplification (WGA), followed by preamplification and amplification of selected target nucleic acids. Methods for analyzing RNA can entail reverse transcription of the desired RNA, followed by preamplification and amplification of selected target nucleic acids.
    Type: Grant
    Filed: December 10, 2013
    Date of Patent: February 2, 2016
    Assignee: Fluidigm Corporation
    Inventors: Amy Hamilton, Min Lin, Alain Mir, Martin Pieprzyk
  • Publication number: 20150362420
    Abstract: The present invention provides methods, device, assemblies, and systems for counting and dispensing single or multiple cells (e.g., into the open wells of a multi-well testing device). In certain embodiments, the systems comprise: a) a fluid movement component composed of upstream and downstream electrode conduits connected to a non-conductive conduit, and b) an electronic signal detector electrically linked to the upstream and downstream electrode conduits such that, when a fluid is present in the fluid movement component, an electrical circuit is established that is altered when a cell passes through the non-conductive conduit. In other embodiments, the systems comprises a) a fluid movement component composed of an upstream electrode conduit connected to a non-conductive conduit, b) an in-well electrode, and c) an electronic signal detector electrically linked to the upstream electrode conduit and the in-well electrode.
    Type: Application
    Filed: June 12, 2015
    Publication date: December 17, 2015
    Inventors: Patricio A. Espinoza Vallejos, Alain Mir, Syed A. Husain, Michael Slater, Maithreyan Srinivasan
  • Publication number: 20150038336
    Abstract: The present invention is directed to methods for identifying the presence of one or more target nucleotide sequences in a sample that involve a nuclease-ligation reaction. In some embodiments, the ligation products formed in the nuclease-ligation process of the present invention are subsequently amplified using a polymerase chain reaction. The ligated product sequences or extension products thereof are detected, and the presence of one or more target nucleotide sequences in the sample is identified based on the detection.
    Type: Application
    Filed: February 14, 2013
    Publication date: February 5, 2015
    Inventors: Francis Barany, Eugene Spier, Alain Mir
  • Patent number: 8921049
    Abstract: The present invention provides for determining relative copy number difference for one or more target nucleic acid sequences between a test sample and a reference sample or reference value derived therefrom. The methods facilitate the detection of copy number differences less than 1.5-fold.
    Type: Grant
    Filed: April 24, 2013
    Date of Patent: December 30, 2014
    Assignee: Fluidigm Corporation
    Inventors: Simant Dube, Alain Mir, Ramesh Ramakrishnan, Lesley Suzanne Weaver, Bernhard G. Zimmermann