Abstract: The present disclosure provides methods for identifying candidate compounds having bone anti-resorption activity or bone pro-formation activity. The methods involve the use of ex vivo-derived mineralized three-dimensional bone constructs. The bone constructs are obtained by culturing osteoblasts and osteoclast precursors under randomized gravity vector conditions in the presence of the candidate compound. Preferably, the randomized gravity vector conditions are obtained using a low shear stress rotating bioreactor, such as a High Aspect Ratio Vessel (HARV) culture system.

Abstract: The present disclosure provides ex vivo-derived mineralized three-dimensional bone constructs. The bone constructs are obtained by culturing osteoblasts and osteoclast precursors under randomized gravity vector conditions. Preferably, the randomized gravity vector conditions are obtained using a low shear stress rotating bioreactor, such as a High Aspect Ratio Vessel (HARV) culture system. The bone constructs of the disclosure have utility in physiological studies of bone formation and bone function, in drug discovery, and in orthopedics.

Abstract: The present disclosure provides colloidal bone graft compositions comprising a plurality of mineralized three-dimensional bone constructs, a polymerizable biocompatible matrix, an osteoblast differentiation agent, and at least one osteoblast mineralization agent. The colloidal bone graft compositions of the disclosure are used to perform bone grafts in human or animal patients.

Abstract: The present disclosure provides ex-vivo derived mineralized three-dimensional bone constructs. The bone constructs are obtained by culturing osteoblasts and osteclast precursors under randomized gravity vector conditions. Preferably, the randomized gravity vector conditions are obtained using a low shear stress rotating bioreactor, such as a High Aspect Ratio Vessel (HARV) culture system. The bone constructs of the disclosure have utility in physiological studies of bone formation and bone function, in drug discovery, and in orthopedics.

Abstract: The present disclosure provides ex vivo-derived mineralized three-dimensional bone constructs. The bone constructs are obtained by culturing osteoblasts and osteoclast precursors under randomized gravity vector conditions. Preferably, the randomized gravity vector conditions are obtained using a low shear stress rotating bioreactor, such as a High Aspect Ratio Vessel (HARV) culture system. The bone constructs of the disclosure have utility in physiological studies of bone formation and bone function, in drug discovery, and in orthopedics.

Abstract: The present disclosure provides colloidal bone graft compositions comprising a plurality of mineralized three-dimensional bone constructs, a polymerizable biocompatible matrix, an osteoblast differentiation agent, and at least one osteoblast mineralization agent. The colloidal bone graft compositions of the disclosure are used to perform bone grafts in human or animal patients.

Abstract: The present disclosure provides methods for identifying candidate compounds having bone anti-resorption activity or bone pro-formation activity. The methods involve the use of ex vivo-derived mineralized three-dimensional bone constructs. The bone constructs are obtained by culturing osteoblasts and osteoclast precursors under randomized gravity vector conditions in the presence of the candidate compound. Preferably, the randomized gravity vector conditions are obtained using a low shear stress rotating bioreactor, such as a High Aspect Ratio Vessel (HARV) culture system.

Abstract: The present disclosure provides methods for producing human osteogenic cell lines in which HLA Class I expression is absent or reduced. The resulting osteogenic cells can then be used, for example, to form mineralized three-dimensional bone constructs which can be used, for example, in bone grafting procedures. The lack of HLA Class I expression in the cells of the mineralized three-dimensional bone constructs reduces or eliminates the potential for host-graft rejection.

Abstract: The present disclosure provides ex vivo-derived human demineralized bone matrix. The bone matrix is produced using mineralized three-dimensional bone constructs. The bone constructs are obtained by culturing osteoblasts and osteoclast precursors under randomized gravity vector conditions. Preferably, the randomized gravity vector conditions are obtained using a low shear stress rotating bioreactor, such as a High Aspect Ratio Vessel (HARV) culture system. The ex vivo-derived human demineralized bone matrix of the disclosure can be used in, for example, orthopedic and periodontal procedures.

Abstract: The present disclosure provides ex vivo-derived mineralized three-dimensional bone constructs. The bone constructs are obtained by culturing osteoblasts under randomized gravity vector conditions. Preferably, the randomized gravity vector conditions are obtained using a low shear stress rotating bioreactor, such as a High Aspect Ratio Vessel (HARV) culture system. The bone constructs of the disclosure have utility in physiological studies of bone formation and bone function, in drug discovery, and in orthopedics.

Abstract: The present disclosure provides methods for producing Bone Morphogenetic Proteins (BMPs). The BMPs are produced using ex vivo-derived mineralized three-dimensional bone constructs of varying degrees of maturity and mineralization. The bone constructs are obtained by culturing osteoblasts and osteoclast precursors under randomized gravity vector conditions whereupon the bone constructs secrete BMPs into the culture medium. Culture medium is periodically removed from the bone construct culture, and the BMPs are purified from the removed culture medium.

Abstract: The present disclosure provides ex vivo-derived mineralized three-dimensional bone constructs. The bone constructs are obtained by culturing osteoblasts and osteoclast precursors under randomized gravity vector conditions. Preferably, the randomized gravity vector conditions are obtained using a low shear stress rotating bioreactor, such as a High Aspect Ratio Vessel (HARV) culture system. The bone constructs of the disclosure have utility in physiological studies of bone formation and bone function, in drug discovery, and in orthopedics.

Abstract: The present disclosure provides ex vivo-derived mineralized three-dimensional bone constructs. The bone constructs are obtained by culturing osteoblasts and osteoclast precursors under randomized gravity vector conditions. Preferably, the randomized gravity vector conditions are obtained using a low shear stress rotating bioreactor, such as a High Aspect Ratio Vessel (HARV) culture system. The bone constructs of the disclosure have utility in physiological studies of bone formation and bone function, in drug discovery, and in orthopedics.