Abstract: A method is provided for producing a sterile and pyrogen-free column containing coupled protein for use in removing a predetermined substance from the blood of a human subject. The method abrogates sterilization of the finished protein-containing product by providing sterile and pyrogen-free raw materials at each production step. The method provides a pathogen-free, purified solution of protein which binds to a predetermined substance in human blood such as LDL or immunoglobulin. Typically, the protein is anti-human LDL immunoglobulin or anti-human Ig immunoglobulin. The method also provides a sterile and pyrogen-free column matrix material such as an agarose which is chemically activated, either using CNBr and triethylamine or using 1,1'-carbonyldiimidazole.

Abstract: A method for displaying terrain features of a topological map incudes the determination of resultant slopes between data points comprising the map. The slopes are represented by different levels of pixel intensity which are displayed upon a raster scan projection device. In an alternate embodiment of the invention, a point source of light is used to illuminate the topological surface. Normals to the surface are determined which indicate how much light would be reflected by the point light source. The amount of light reflected is then determined for each of the points of the map. The different light intensities are graded into various shades or intensities of light and displayed upon a raster display device.