Abstract: The present invention relates to the production of the receptor binding domain (RBD) of the Spike glycoprotein 1 of the SARS-CoV-2 in mammalian cell expression systems, and the successive method of purification thereof. A recombinant plasmid containing the coding sequence of said RBD is produced and transfected in said mammalian cells, for example, Expi293. A high level of the protein is secreted in the medium and subsequently purified using the N-terminal tag, that can be removed by a specific protease. The present invention also includes a recombinant expression vector carrying the RBD gene, the successive methods for protein purification, the strategy for establishing a stable cell line producing the RBD, methods of use of the recombinant protein in formulating a pharmaceutical composition, including but not limited to, vaccines for preventing SARS-CoV-2 induced diseases.

Abstract: The present invention relates to the production of the receptor binding domain (RBD) of the Spike glycoprotein 1 of the SARS-CoV-2 in mammalian cell expression systems, and the successive method of purification thereof. A recombinant plasmid containing the coding sequence of said RBD is produced and transfected in said mammalian cells, for example, Expi293. A high level of the protein is secreted in the medium and subsequently purified using the N-terminal tag, that can be removed by a specific protease. The present invention also includes a recombinant expression vector carrying the RBD gene, the successive methods for protein purification, the strategy for establishing a stable cell line producing the RBD, methods of use of the recombinant protein in formulating a pharmaceutical composition, including but not limited to, vaccines for preventing SARS-CoV-2 induced diseases.

Abstract: The present invention relates to the production of the receptor binding domain (RBD) of the Spike glycoprotein 1 of the SARS-CoV-2 in mammalian cell expression systems, and the successive method of purification thereof. A recombinant plasmid containing the coding sequence of said RBD is produced and transfected in said mammalian cells, for example, Expi293. A high level of the protein is secreted in the medium and subsequently purified using the N-terminal tag, that can be removed by a specific protease. The present invention also includes a recombinant expression vector carrying the RBD gene, the successive methods for protein purification, the strategy for establishing a stable cell line producing the RBD, methods of use of the recombinant protein in formulating a pharmaceutical composition, including but not limited to, vaccines for preventing SARS-CoV-2 induced diseases.

Abstract: Provided herein are computer-implemented methods of identifying programmed-death ligand 1 (PD-L1) expression status of a subject's sample comprising a cancer cell. In exemplary embodiments, the method comprises receiving an unlabeled expression data set for the subject's sample; aligning the unlabeled expression data set to labeled expression data according to a trained PD-L1 predictive model, wherein the trained PD-L1 predictive model has been trained with a plurality of labeled expression data sets, each labeled expression data set comprising expression data for a sample of a labeled cancer type and a labeled PD-L1 expression status; wherein aligning the unlabeled gene expression data set to labeled expression data according to the trained PD-L1 predictive model identifies PD-L1 expression status for the subject's sample. Further provided are related methods of preparing a clinical decision support information (CDSI) report and methods of determining treatment for a subject.

Abstract: The present disclosure relates in general to devices, systems and methods for wireless communication, and in particular to communication using a proximity integrated circuit card (PICC). Example embodiments include a circuit (100) for a PICC, the circuit comprising an input stage (101), a decoding module (106) and a bias adjustment module (117), the bias adjustment module (117) configured to receive an output code from the decoding module and provide a bias adjustment signal to the input stage (101), the bias adjustment module (117) configured to iteratively tune the bias adjustment signal based on a measurement of the output code, with successive steps tuning the bias adjustment signal by a smaller amount until the output code is within a decoding range.

Abstract: The present disclosure relates in general to devices, systems and methods for wireless communication, and in particular to communication using a proximity integrated circuit card (PICC). Example embodiments include a circuit (100) for a PICC, the circuit comprising an input stage (101), a decoding module (106) and a bias adjustment module (117), the bias adjustment module (117) configured to receive an output code from the decoding module and provide a bias adjustment signal to the input stage (101), the bias adjustment module (117) configured to iteratively tune the bias adjustment signal based on a measurement of the output code, with successive steps tuning the bias adjustment signal by a smaller amount until the output code is within a decoding range.

Abstract: A method in a mobile satellite positioning system receiver operating in cold-start mode, including searching for and detecting (310) a first satellite in a constellation of orbiting satellites, searching (320) for a second satellite based on detection probability information for non-detected satellites, the detection probability information obtained using information about one of satellites not detected or the first satellite detected. In one embodiment, the detection probability information is based on a measure of range between the non-detected satellites and the first satellite detected, and in another embodiment the detection probability information is based on a measure of angle between the non-detected satellites and the first satellite detected.

Abstract: A method in a mobile satellite positioning system receiver operating in cold-start mode, including searching for and detecting (310) a first satellite in a constellation of orbiting satellites, searching (320) for a second satellite based on detection probability information for non-detected satellites, the detection probability information obtained using information about one of satellites not detected or the first satellite detected. In one embodiment, the detection probability information is based on a measure of range between the non-detected satellites and the first satellite detected, and in another embodiment the detection probability information is based on a measure of angle between the non-detected satellites and the first satellite detected.