Patents by Inventor Alan Collmer
Alan Collmer has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9689012Abstract: The present invention relates to methods of assembling a plurality of genetic units to form synthetic genetic constructs. This method involves appending universal adapter oligonucleotides and flexible adapter oligonucleotides to the 5? and 3? ends of separate genetic units to be assembled to form separate dual extended genetic units. The dual extended genetic units are assembled together via homologous recombination between the flexible adapter oligonucleotide portions of the dual extended units to form synthetic genetic constructs. The present invention further relates to synthetic genetic constructs formed using the methods of the present invention, and vectors, cells, and organisms containing such synthetic genetic constructs.Type: GrantFiled: October 12, 2011Date of Patent: June 27, 2017Assignee: Cornell UniversityInventors: Sébastien Cunnac, Alan Collmer
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Patent number: 8716460Abstract: One aspect of the present invention relates to isolated nucleic acid molecules encoding avirulence proteins or polypeptides of Pseudomonas syringae pv. syringae DC 3000, or nucleic acid molecules which are complementary thereto. Expression vectors, host cells, and transgenic plants which include the DNA molecules of the present invention are also disclosed. Another aspect relates to the isolated proteins or polypeptides and compositions containing the same. The various nucleic acid molecules and proteins of the present invention can be used to impart disease resistance to a plant, make a plant hypersusceptible to colonization by nonpathogenic bacteria, modify a metabolic pathway in a cell, cause eukaryotic cell death and treat a cancerous condition, as well as inhibit programmed cell death.Type: GrantFiled: December 27, 2006Date of Patent: May 6, 2014Assignees: Kansas State University Research Foundation, The Board of Regents of the University of Nebraska, Cornell Research Foundation, Inc., The United States of America as Represented by the Secretary of AgricultureInventors: James R. Alfano, Alan Collmer, Samuel W. Cartinhour, David J. Schneider, Xiaoyan Tang
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Publication number: 20130298265Abstract: The present invention relates to methods of assembling a plurality of genetic units to form synthetic genetic constructs. This method involves appending universal adapter oligonucleotides and flexible adapter oligonucleotides to the 5? and 3? ends of separate genetic units to be assembled to form separate dual extended genetic units. The dual extended genetic units are assembled together via homologous recombination between the flexible adapter oligonucleotide portions of the dual extended units to form synthetic genetic constructs. The present invention further relates to synthetic genetic constructs formed using the methods of the present invention, and vectors, cells, and organisms containing such synthetic genetic constructs.Type: ApplicationFiled: October 12, 2011Publication date: November 7, 2013Applicant: CORNELL UNIVERSITYInventors: Sébastien Cunnac, Alan Collmer
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Publication number: 20070162994Abstract: One aspect of the present invention relates to isolated nucleic acid molecules encoding avirulence proteins or polypeptides of Pseudomonas syringae pv. syringae DC 3000, or nucleic acid molecules which are complementary thereto. Expression vectors, host cells, and transgenic plants which include the DNA molecules of the present invention are also disclosed. Another aspect relates to the isolated proteins or polypeptides and compositions containing the same. The various nucleic acid molecules and proteins of the present invention can be used to impart disease resistance to a plant, make a plant hypersusceptible to colonization by nonpathogenic bacteria, modify a metabolic pathway in a cell, cause eukaryotic cell death and treat a cancerous condition, as well as inhibit programmed cell death.Type: ApplicationFiled: December 27, 2006Publication date: July 12, 2007Inventors: Alan Collmer, James Alfano, Samuel Cartinhour, David Schneider, Xiaoyan Tang
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Patent number: 7220583Abstract: One aspect of the present invention relates to isolated nucleic acid molecules encoding avirulence proteins or polypeptides of Pseudomonas syringae pv. syringae DC 3000, or nucleic acid molecules which are complementary thereto. Expression vectors, host cells, and transgenic plants which include the DNA molecules of the present invention are also disclosed. Another aspect relates to the isolated proteins or polypeptides and compositions containing the same. The various nucleic acid molecules and proteins of the present invention can be used to impart disease resistance to a plant, make a plant hypersusceptible to colonization by nonpathogenic bacteria, modify a metabolic pathway in a cell, cause eukaryotic cell death and treat a cancerous condition, as well as inhibit programmed cell death.Type: GrantFiled: February 12, 2003Date of Patent: May 22, 2007Assignees: Cornell Research Foundation, Inc., The Board of Regents of the University of Nebraska, The United States of America, as Represented by the Secretary of AgricultureInventors: James R. Alfano, Alan Collmer, Samuel W. Cartinhour, David J. Schneider
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Patent number: 7138569Abstract: The present invention relates to isolated nucleic acid molecules encoding a type III—secreted bacterial protein capable of modifying a cell death pathway in a plant cell. One aspect of the present invention involves an isolated nucleic acid molecule having a nucleotide sequence that encodes the HopPtoD2 protein of Pseudomonas syringae pv. syringae DC 3000. Expression vectors, host cells, and transgenic plants which include the DNA molecules of the present invention are also disclosed. The nucleic acid molecules of the present invention can be used to impart disease resistance to a plant and to make a plant hypersusceptible to colonization by nonpathogenic bacteria.Type: GrantFiled: April 2, 2002Date of Patent: November 21, 2006Assignees: Cornell Research Foundation, Inc., Kansas State University Research Foundation, Boyce Thompson Institute for Plant Research, Inc., The Institute for Genomic Research, The Board of Regents of the University of NebraskaInventors: Alan Collmer, James R. Alfano, Xiaoyan Tang, C. Robin Buell, Gregory B. Martin
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Patent number: 7109397Abstract: The present invention relates to isolated DNA molecules that encode proteins or polypeptides which elicit a hypersensitive response in plants. One aspect of the present invention involves an isolated DNA molecule that encodes the HopPtoP protein of Pseudomonas syringae pv. tomato DC3000. The isolated DNA molecules can be used to impart disease resistance, stress resistance, and enhanced growth to plants or plants grown from treated seeds, to control insects on plants or plants grown from treated plant seeds, to impart post-harvest disease or desiccation resistance in fruits or vegetables, to impart enhanced longevity of fruit or vegetable ripeness, to impart desiccation resistance to cuttings of ornamental plants, and/or promote early flowering of ornamental plants, either by topical application of the proteins or polypeptides or transgenic expression in recombinant plants or plant seeds.Type: GrantFiled: January 30, 2003Date of Patent: September 19, 2006Assignee: Cornell Research Foundation, Inc.Inventors: Alan Collmer, Adela Ramos
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Patent number: 7102059Abstract: One aspect of the present invention relates to isolated nucleic acid molecules (i) encoding proteins or polypeptides of Pseudomonas CEL and EEL genomic regions, (ii) nucleic acid molecules which hybridize thereto under stringent conditions, or (iii) nucleic acid molecules that include a nucleotide sequence which is complementary to the nucleic acid molecules of (i) and (ii). Expression vectors, host cells, and transgenic plants which include the DNA molecules of the present invention are also disclosed. Another aspect relates to the isolated proteins or polypeptides and compositions containing the same. The nucleic acid molecules and proteins of the present invention can be used to imparting disease resistance to a plant, making a plant hypersusceptible to colonization by nonpathogenic bacteria, causing eukaryotic cell death, and treating cancerous conditions.Type: GrantFiled: July 16, 2004Date of Patent: September 5, 2006Assignees: Cornell Research Foundation, Inc., Board of Regents University and Community College System of Nevada, on Behalf of the University of Nevada, Las Vegas, University of Nebraska at LincolnInventors: Alan Collmer, James R. Alfano, Amy O. Charkowski
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Patent number: 7045123Abstract: The present invention is directed to an a isolated DNA molecule from Pseudomonas syringae that encodes a protein or polypeptide which elicits a hypersensitive response in plants. This isolated DNA molecule can used to impart disease resistance to plants, to enhance plant growth, and/or to control insects on plants. Plants or plant seeds transformed with a DNA molecule encoding a hypersensitive response elicitor protein or polypeptide can be provided, and the transgenic plants or plants resulting from the transgenic plant seeds are grown under conditions effective to impart disease resistance, to enhance plant growth, and/or to control insects on plants or plants grown from the plant seeds.Type: GrantFiled: June 20, 2000Date of Patent: May 16, 2006Assignee: Cornell Research Foundation, Inc.Inventors: Alan Collmer, Amy Charkowski, James R. Alfano
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Publication number: 20050120409Abstract: The present invention is directed to isolated proteins or polypeptides which elicit a hypersensitive response in plants, as well as isolated DNA molecules which encode the hypersensitive response eliciting proteins or polypeptides. These isolated proteins or polypeptides and the isolated DNA molecules can be used to impart disease resistance, stress resistance, and enhanced growth to plants or plants grown from treated seeds, to control insects on plants or plants grown from treated plant seeds, to impart post-harvest disease or desiccation resistance in fruits or vegetables, to impart enhanced longevity of fruit or vegetable ripeness, to impart desiccation resistance to cuttings of ornamental plants, and/or promote early flowering of ornamental plants, either by topical application of the proteins or polypeptides or transgenic expression in recombinant plants or plant seeds.Type: ApplicationFiled: January 30, 2003Publication date: June 2, 2005Inventors: Alan Collmer, Adela Ramos
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Publication number: 20050039232Abstract: One aspect of the present invention relates to isolated nucleic acid molecules (i) encoding proteins or polypeptides of Pseudomonas CEL and EEL genomic regions, (ii) nucleic acid molecules which hybridize thereto under stringent conditions, or (iii) nucleic acid molecules that include a nucleotide sequence which is complementary to the nucleic acid molecules of (i) and (ii). Expression vectors, host cells, and transgenic plants which include the DNA molecules of the present invention are also disclosed. Another aspect relates to the isolated proteins or polypeptides and compositions containing the same. The nucleic acid molecules and proteins of the present invention can be used to imparting disease resistance to a plant, making a plant hypersusceptible to colonization by nonpathogenic bacteria, causing eukaryotic cell death, and treating cancerous conditions.Type: ApplicationFiled: July 16, 2004Publication date: February 17, 2005Inventors: Alan Collmer, James Alfano, Amy Charkowski
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Patent number: 6852835Abstract: One aspect of the present invention relates to isolated nucleic acid molecules (i) encoding proteins or polypeptides of Pseudomonas CEL and EEL genomic regions, (ii) nucleic acid molecules which hybridize thereto under stringent conditions, or (iii) nucleic acid molecules that include a nucleotide sequence which is complementary to the nucleic acid molecules of (i) and (ii). Expression vectors, host cells, and transgenic plants which include the DNA molecules of the present invention are also disclosed. Another aspect relates to the isolated proteins or polypeptides and compositions containing the same. The nucleic acid molecules and proteins of the present invention can be used to imparting disease resistance to a plant, making a plant hypersusceptible to colonization by nonpathogenic bacteria, causing eukaryotic cell death, and treating cancerous conditions.Type: GrantFiled: April 3, 2001Date of Patent: February 8, 2005Inventors: Alan Collmer, James R. Alfano, Amy O. Charkowski
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Publication number: 20040006789Abstract: The present invention is directed to isolated proteins or polypeptides which elicit a hypersensitive response in plants, as well as isolated DNA molecules which encode the hypersensitive response eliciting proteins or polypeptides. These isolated proteins or polypeptides and the isolated DNA molecules can be used to impart disease resistance, stress resistance, and enhanced growth to plants or plants grown from treated seeds, to control insects on plants or plants grown from treated plant seeds, to impart post-harvest disease or desiccation resistance in fruits or vegetables, to impart enhanced longevity of fruit or vegetable ripeness, to impart desiccation resistance to cuttings of ornamental plants, and/or promote early flowering of ornamental plants, either by topical application of the proteins or polypeptides or transgenic expression in recombinant plants or plant seeds.Type: ApplicationFiled: January 30, 2003Publication date: January 8, 2004Inventors: Alan Collmer, Adela Ramos
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Publication number: 20030204868Abstract: One aspect of the present invention relates to isolated nucleic acid molecules encoding avirulence proteins or polypeptides of Pseudomonas syringae pv. syringae DC 3000, or nucleic acid molecules which are complementary thereto. Expression vectors, host cells, and transgenic plants which include the DNA molecules of the present invention are also disclosed. Another aspect relates to the isolated proteins or polypeptides and compositions containing the same. The various nucleic acid molecules and proteins of the present invention can be used to impart disease resistance to a plant, make a plant hypersusceptible to colonization by nonpathogenic bacteria, modify a metabolic pathway in a cell, cause eukaryotic cell death and treat a cancerous condition, as well as inhibit programmed cell death.Type: ApplicationFiled: February 12, 2003Publication date: October 30, 2003Inventors: Alan Collmer, James R. Alfano, Samuel W. Cartinhour, David J. Schneider, Xiaoyan Tang
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Publication number: 20030182681Abstract: One aspect of the present invention relates to isolated nucleic acid molecules encoding avirulence proteins or polypeptides of Pseudomonas syringae pv. syringae DC 3000, or nucleic acid molecules which are complementary thereto. Expression vectors, host cells, and transgenic plants which include the DNA molecules of the present invention are also disclosed. Another aspect relates to the isolated proteins or polypeptides and compositions containing the same. The nucleic acid molecules and proteins of the present invention can be used to impart disease resistance to a plant.Type: ApplicationFiled: April 2, 2002Publication date: September 25, 2003Inventors: Alan Collmer, James R. Alfano, Xiaoyan Tang, C. Robin Buell, Gregory B. Martin
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Patent number: 6596509Abstract: One aspect of the present invention relates to a DNA construct that contains a first DNA molecule encoding a functional type III secretion system, a promoter, and a second DNA molecule encoding a protein or polypeptide capable of being secreted by the type III secretion system. The second DNA molecule is operably coupled to the promoter so that upon introduction of the DNA construct into a host cell, the encoded protein or polypeptide and the type III secretion system are expressed and the encoded protein or polypeptide is secreted. Another aspect of the present invention relates to a system that includes (i) a first DNA construct having a first DNA molecule encoding a functional type III secretion system and (ii) a second DNA construct having a promoter operably coupled to a second DNA molecule encoding a protein or polypeptide capable of being secreted by the type III secretion system.Type: GrantFiled: July 9, 1999Date of Patent: July 22, 2003Assignee: Cornell Research Foundation, Inc.Inventors: David W. Bauer, Steven V. Beer, Adam J. Bogdanove, Alan Collmer, Jong Hyun Ham
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Patent number: 6485977Abstract: The present invention relates to a method for delivering effector proteins into a target cell. This method involves introducing into the target cell an effector protein fused to a protein transduction domain of a human immunodeficiency virus TAT protein or derivatives or functional analogs thereof. The present invention also relates to a fusion protein including an effector protein fused to a protein transduction domain of a human immunodeficiency virus TAT protein or derivatives or functional analogs thereof. Another aspect of the present invention relates to a DNA construct including a first DNA molecule encoding an effector protein and a second DNA molecule operatively associated with the first DNA molecule and encoding a protein transduction domain of a human immunodeficiency virus TAT protein or derivatives or functional analogs thereof and its use in a method for delivering effector proteins into a target cell.Type: GrantFiled: September 13, 2000Date of Patent: November 26, 2002Assignee: Cornell Research Foundation, Inc.Inventors: Alan Collmer, Steven V. Beer
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Publication number: 20020083489Abstract: One aspect of the present invention relates to isolated nucleic acid molecules (i) encoding proteins or polypeptides of Pseudomonas CEL and EEL genomic regions, (ii) nucleic acid molecules which hybridize thereto under stringent conditions, or (iii) nucleic acid molecules that include a nucleotide sequence which is complementary to the nucleic acid molecules of (i) and (ii). Expression vectors, host cells, and transgenic plants which include the DNA molecules of the present invention are also disclosed. Another aspect relates to the isolated proteins or polypeptides and compositions containing the same. The nucleic acid molecules and proteins of the present invention can be used to imparting disease resistance to a plant, making a plant hypersusceptible to colonization by nonpathogenic bacteria, causing eukaryotic cell death, and treating cancerous conditions.Type: ApplicationFiled: April 3, 2001Publication date: June 27, 2002Inventors: Alan Collmer, James R. Alfano, Amy O. Charkowski
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Patent number: 6174717Abstract: The nucleic acid and amino acid sequences for proteinaceous elicitors of the plant defense reaction known as the hypersensitive response are described along with methods for preparation and processes for inactivation.Type: GrantFiled: May 5, 1997Date of Patent: January 16, 2001Assignee: Cornell Research Foundation, Inc.Inventors: Steven V. Beer, Zhong-Min Wei, David W. Bauer, Alan Collmer, Sheng-Yang He, Ron Laby
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Patent number: 6172184Abstract: The present invention is directed to an isolated protein or polypeptide which elicits a hypersensitive response in plants as well as an isolated DNA molecule which encodes the hypersensitive response eliciting protein or polypeptide. This isolated protein or polypeptide and the isolated DNA molecule can used to impart disease resistance to plants, to enhance plant growth, and/or to control insects on plants. This can be achieved by applying the hypersensitive response elicitor protein or polypeptide in a non-infectious form to plants or plant seeds under conditions effective to impart disease resistance, to enhance plant growth, and/or to control insects on plants or plants grown from the plant seeds.Type: GrantFiled: July 22, 1998Date of Patent: January 9, 2001Assignee: Cornell Research Foundation, Inc.Inventors: Alan Collmer, Amy Charkowski, James R. Alfano