Abstract: Methods and reagents are disclosed for pretreating a sample suspected of containing a hydrophobic drug for conducting an assay method for detecting the hydrophobic drug. A combination is provided in a medium that includes the sample, a releasing agent for releasing the hydrophobic drug and the metabolites from endogenous binding moieties, and a selective solubility agent that provides for substantially equal solubility of the hydrophobic drug and the metabolites in the medium. The selective solubility agent includes a water miscible, non-volatile organic solvent and is present in the medium in a concentration sufficient to provide for substantially equal solubility of the hydrophobic drug and the metabolites in the medium. The medium, which may further include a hemolytic agent, is incubated under conditions for releasing the hydrophobic drug and the metabolites from endogenous binding moieties. The pretreated sample may be subjected to an assay for determining the hydrophobic drug.

Abstract: Methods and reagents are disclosed for pretreating a sample suspected of containing a hydrophobic drug for conducting an assay method for detecting the hydrophobic drug. A combination is provided in a medium that includes the sample, a releasing agent for releasing the hydrophobic drug and the metabolites from endogenous binding moieties, and a selective solubility agent that provides for substantially equal solubility of the hydrophobic drug and the metabolites in the medium. The selective solubility agent includes a water miscible, non-volatile organic solvent and is present in the medium in a concentration sufficient to provide for substantially equal solubility of the hydrophobic drug and the metabolites in the medium. The medium, which may further include a hemolytic agent, is incubated under conditions for releasing the hydrophobic drug and the metabolites from endogenous binding moieties. The pretreated sample may be subjected to an assay for determining the hydrophobic drug.

Abstract: Methods and reagents are disclosed for pretreating a sample suspected of containing a hydrophobic drug for conducting an assay method for detecting the hydrophobic drug. A combination is provided in a medium. The combination comprises (i) the sample, (ii) a releasing agent for releasing the hydrophobic drug and the metabolites from endogenous binding moieties, and (iii) a selective solubility agent that provides for substantially equal solubility of the hydrophobic drug and the metabolites in the medium. The selective solubility agent comprises a water miscible, non-volatile organic solvent and is present in the medium in a concentration sufficient to provide for substantially equal solubility of the hydrophobic drug and the metabolites in the medium. The medium, which may further comprise a hemolytic agent, is incubated under conditions for releasing the hydrophobic drug and the metabolites from endogenous binding moieties.

Abstract: Methods and reagents are disclosed for pretreating a sample suspected of containing a hydrophobic drug for conducting an assay method for detecting the hydrophobic drug. A combination is provided in a medium. The combination comprises (i) the sample, (ii) a releasing agent for releasing the hydrophobic drug and the metabolites from endogenous binding moieties, and (iii) a selective solubility agent that provides for substantially equal solubility of the hydrophobic drug and the metabolites in the medium. The selective solubility agent comprises a water miscible, non-volatile organic solvent and is present in the medium in a concentration sufficient to provide for substantially equal solubility of the hydrophobic drug and the metabolites in the medium. The medium, which may further comprise a hemolytic agent, is incubated under conditions for releasing the hydrophobic drug and the metabolites from endogenous binding moieties.

Abstract: An editing system that allows a user, when browsing the web, to select a web page, which is currently being viewed, to be edited. When a user navigates to a web page that the user can edit, the editing system displays an appropriate indication that the page can be edited. When the user selects to edit the currently displayed web page, the editing system enters an editing mode that allows the user to edit the web page within a browsing context. When in editing mode, the editing system may enable various toolbars and buttons within the window of the browser to facilitate the editing. When the user has completed the editing of the web page, the editing system automatically updates the web page at the web site.

Abstract: A method and system for allowing end users to create a database and a user interface to interact with database data are provided. The database management system provides a database abstraction layer that hides the complexities of a database and vendor-specific implementation details from the end user, while still allowing the end user to perform powerful database tasks. The database management system allows an end user with little or no knowledge about databases to create, edit, and maintain a database without understanding the details behind the database. The database management system also provides a facility for the easy creation of web pages to access the database to provide features such as searching, data entry, and browsing data stored in the database.

Abstract: A method and system for augmenting a corpus with documents on concepts not sufficiently covered within the corpus is provided. The augmentation system generates a corpus concept graph from the documents of a corpus. A corpus concept graph represents concepts of the documents as nodes and related concepts as links between nodes. To generate a corpus concept graph, the augmentation system identifies the concepts that are related within each document of the corpus and adds nodes and links to the corpus concept graph for related concepts. The augmentation system analyzes the corpus concept graph to determine whether the relatedness of concepts of the documents of the corpus is sufficient. If the relatedness of a pair of concepts is not sufficient, then the augmentation system attempts to identify documents not already in the corpus that are related to the concepts that are not sufficiently related.

Abstract: Methods and reagents are disclosed for pretreating a sample suspected of containing a hydrophobic drug for conducting an assay method for detecting the hydrophobic drug. A combination is provided in a medium. The combination comprises (i) the sample, (ii) a releasing agent for releasing the hydrophobic drug and the metabolites from endogenous binding moieties, and (iii) a selective solubility agent that provides for substantially equal solubility of the hydrophobic drug and the metabolites in the medium. The selective solubility agent comprises a water miscible, non-volatile organic solvent and is present in the medium in a concentration sufficient to provide for substantially equal solubility of the hydrophobic drug and the metabolites in the medium. The medium, which may further comprise a hemolytic agent, is incubated under conditions for releasing the hydrophobic drug and the metabolites from endogenous binding moieties.

Abstract: Methods and compositions are disclosed for reducing non-specific binding in a binding assay for the determination of an analyte in a sample where one of the reagents for conducting the binding assay comprises a solid support comprising a polysaccharide. The method comprises including in an assay medium for conducting the binding assay a soluble compound comprising a protein linked to a polysaccharide. Also disclosed are methods and compositions for determining the presence and/or amount of an analyte in a sample suspected of containing the analyte. The methods include as reagents a solid support comprising a polysaccharide and a soluble compound comprising a protein linked to a polysaccharide.

Abstract: A method and system for augmenting a corpus with documents on concepts not sufficiently covered within the corpus is provided. The augmentation system generates a corpus concept graph from the documents of a corpus. A corpus concept graph represents concepts of the documents as nodes and related concepts as links between nodes. To generate a corpus concept graph, the augmentation system identifies the concepts that are related within each document of the corpus and adds nodes and links to the corpus concept graph for related concepts. The augmentation system analyzes the corpus concept graph to determine whether the relatedness of concepts of the documents of the corpus is sufficient. If the relatedness of a pair of concepts is not sufficient, then the augmentation system attempts to identify documents not already in the corpus that are related to the concepts that are not sufficiently related.

Abstract: Compositions suitable for use as signal generation components of an immunoassay, and methods for their use. According to one aspect of the invention, the composition includes a carrier having a coating of an aminodextran and a metal chelate incorporated therein. The metal chelate is present in the amount of at least 0.065 ?M per milligram of carrier, and the aminodextran coating density averaging at least about 45 ?g per milligram of carrier. In another aspect of the invention, carrier is dyed with a complex having the formula: M(L1)x(L2)y, wherein M is a metal selected from the group consisting of europium, terbium, dysprosium, samarium, osmium and ruthenium; L1 is a ligand selected from the group consisting of DPP, TOPO, TPPO; L2 comprises a ligand having the formula wherein R is one or more substituents, each substituent comprising an electron donating group; n=2-10; x=1-2; and y=2-4.

Abstract: A method and system for allowing end users to create a database and a user interface to interact with database data are provided. The database management system provides a database abstraction layer that hides the complexities of a database and vendor-specific implementation details from the end user, while still allowing the end user to perform powerful database tasks. The database management system allows an end user with little or no knowledge about databases to create, edit, and maintain a database without understanding the details behind the database. The database management system also provides a facility for the easy creation of web pages to access the database to provide features such as searching, data entry, and browsing data stored in the database.

Abstract: Compositions suitable for use as signal generation components of an immunoassay, and methods for their use. According to one aspect of the invention, the composition includes a carrier having a coating of an aminodextran and a metal chelate incorporated therein. The metal chelate is present in the amount of at least 0.065 ?M per milligram of carrier, and the aminodextran coating density averaging at least about 45 ?g per milligram of carrier. In another aspect of the invention, carrier is dyed with a complex having the formula: M (L1)x(L2)y, wherein M is a metal selected from the group consisting of europium, terbium, dysprosium, samarium, osmium and ruthenium; L1 is a ligand selected from the group consisting of DPP, TOPO, TPPO; L2 comprises a ligand having the formula wherein R is one or more substituents, each substituent comprising an electron donating group; n=2?10; x=1?2; and y=2?4.

Abstract: Methods and compositions are disclosed for reducing non-specific binding in a binding assay for the determination of an analyte in a sample where one of the reagents for conducting the binding assay comprises a solid support comprising a polysaccharide. The method comprises including in an assay medium for conducting the binding assay a soluble compound comprising a protein linked to a polysaccharide. Also disclosed are methods and compositions for determining the presence and/or amount of an analyte in a sample suspected of containing the analyte. The methods include as reagents a solid support comprising a polysaccharide and a soluble compound comprising a protein linked to a polysaccharide.

Abstract: In some embodiments, the present invention pertains to a method for conjugating a first compound to a second compound wherein the conjugation involves an electrophilic moiety. The method comprises reacting the first compound with the second compound to form a conjugate. The improvement in embodiments of the present invention comprises adding a nucleophilic reagent to the conjugate wherein the nucleophilic reagent forms a neutral product upon reaction with unreacted electrophilic moieties of the conjugate. In some embodiments, the nucleophilic reagent is substantially non-reactive with disulfide bonds in the event that the conjugate comprises disulfide bonds. The conjugate formed is doubly deactivated because the other moiety for linking to the electrophilic moiety is also deactivated.

Abstract: An editing system that allows a user, when browsing the web, to select a web page, which is currently being viewed, to be edited. When a user navigates to a web page that the user can edit, the editing system displays an appropriate indication that the page can be edited. When the user selects to edit the currently displayed web page, the editing system enters an editing mode that allows the user to edit the web page within a browsing context. When in editing mode, the editing system may enable various toolbars and buttons within the window of the browser to facilitate the editing. When the user has completed the editing of the web page, the editing system automatically updates the web page at the web site.

Abstract: An editing system that allows a user, when browsing the web, to select a web page, which is currently being viewed, to be edited. When a user navigates to a web page that the user can edit, the editing system displays an appropriate indication that the page can be edited. When the user selects to edit the currently displayed web page, the editing system enters an editing mode that allows the user to edit the web page within a browsing context. When in editing mode, the editing system may enable various toolbars and buttons within the window of the browser to facilitate the editing. When the user has completed the editing of the web page, the editing system automatically updates the web page at the web site.