Abstract: A remote monitoring system configured to non-invasively measure a cell culture is provided. The system includes a plurality of cell culture layers comprising a cell culture chamber configured to operate as a closed-system, the at least one cell culture chamber having at least one surface to which cells adhere. The system further includes at least one monitoring layer comprising an outer wall surrounding a monitoring layer cell culture chamber configured to operate as a closed-system and having at least one surface to which cells adhere, the at least one monitoring layer comprising at least one indentation in the outer wall. The system also includes at least one monitoring module disposed in at least one of the at least one indentation and comprising at least one of a confluence monitor and an analyte monitor.