Abstract: This document provides fibrin hydrogels and methods and materials for making and using fibrin hydrogels. For example, this document provides fibrin hydrogels containing trypan blue (TB), Evans blue (EB), and/or one or more isomers thereof. Methods for making and using fibrin hydrogels containing TB, EB, and/or one or more isomers thereof also are provided.

Abstract: This document provides methods and materials for performing retinal pigment epithelium transplantation. For example, methods and materials for using fibrin supports for retinal pigment epithelium transplantation are provided.

Abstract: This document provides methods and materials for performing retinal pigment epithelium transplantation. For example, methods and materials for using fibrin supports for retinal pigment epithelium transplantation are provided.

Abstract: This document relates to an implantation device for cellular therapeutics and other therapeutic materials embedded on or in human fibrin or other polymer scaffolds. For example, this document relates to methods and devices for delivering iPSC-RPE monolayers grown on a fibrin scaffold into the sub-retinal space of the eye. The device includes a handle assembly including an actuator and a tip assembly at a distal end of the handle assembly. The tip assembly can house the implant, and the actuator is configured to eject the implant from the tip assembly.

Abstract: This document provides RPE cells and RPE monolayers. For example, compositions containing RPE cells or RPE monolayers as well as methods and materials for making RPE cells or RPE monolayers from, for example, stem cells (e.g., iPSCs) are provided.

Abstract: This document provides methods and materials for performing retinal pigment epithelium transplantation. For example, methods and materials for using fibrin supports for retinal pigment epithelium transplantation are provided.

Abstract: This disclosure concerns methods for identifying Best2 modulators, for example methods of screening for Best2 inhibitors. In some examples, the methods include identifying compounds that alter intracellular calcium concentration, intracellular pH, or transepithelial potential in cells expressing Best2. In certain embodiments, the disclosure concerns methods of identifying compounds that decrease intraocular pressure.

Abstract: An optical scanning spectroscopic apparatus includes a light source and optics to direct light into a region of interest. Means for detecting autofluorescent emissions at a plurality of detectably distinct wavelengths are also provided. A processor receives data corresponding to the autofluorescence and compares the data to a control data set. The light source may alternately include any source of suitable light such as an arc lamp, a laser, or a pulsed laser each controlled to produce a defined wavelength. The comparison of autoflourescent emissions collected at different wavelengths is claimed as a means for diagnosing various retinal diseases.