Abstract: The present invention is related to the field of CRISPR-Cas9 gene editing platforms. In particular, the present invention has identified Type II-C Cas9 anti-CRISPR (Acr) inhibitors that control Cas9 gene editing activity. Co-administration of such Acr inhibitors may provide an advantageous adjunct in permitting safe and practical biological therapeutics through spatial or temporal control of Cas9 activity; controlling Cas9-based gene drives in wild populations to reduce the ecological consequences of such forced inheritance schemes; and contributing to general research into various biotechnological, agricultural, and medical applications of gene editing technologies.

Abstract: The present invention is related to the field of CRISPR-Cas9 gene editing platforms. In particular, the present invention has identified Type II-C Cas9 anti-CRISPR (Acr) inhibitors that control Cas9 gene editing activity. Co-administration of such Acr inhibitors may provide an advantageous adjunct in permitting safe and practical biological therapeutics through spatial or temporal control of Cas9 activity; controlling Cas9-based gene drives in wild populations to reduce the ecological consequences of such forced inheritance schemes; and contributing to general research into various biotechnological, agricultural, and medical applications of gene editing technologies.

Abstract: The present disclosure provides compositions and methods for introducing or enhancing Aca activity in prokaryotic cells. The provided compositions and methods can be used to inhibit Acr activity in prokaryotic cells, thereby enhancing endogenous or exogenous CRISPR-Cas activity. Cells, polynucleotides, plasmids, phage, and other elements for practicing the present methods are also provided.

Abstract: The present invention is related to the field of CRISPR-Cas9 gene editing platforms. In particular, the present invention has identified Type II-C Cas9 anti-CRISPR (Acr) inhibitors that control Cas9 gene editing activity. Co-administration of such Acr inhibitors may provide an advantageous adjunct in permitting safe and practical biological therapeutics through spatial or temporal control of Cas9 activity; controlling Cas9-based gene drives in wild populations to reduce the ecological consequences of such forced inheritance schemes; and contributing to general research into various biotechnological, agricultural, and medical applications of gene editing technologies.

Abstract: One or more keywords associated with a domain are received. The performance of content selected based on the one or more keywords is evaluated against performance of content selected based on a automatic selection process. A determination is made based on the evaluation as to whether to utilize the automatic selection process or the one or more keywords for selecting content for the domain.

Abstract: Several embodiments provide methods and apparatuses for use with node-based compositing to assist in the generation of a second eye or camera of a stereoscopic image. In one implementation, a method for use in 3D movie production comprises the steps: displaying composite nodes to a user, the composite nodes indicating how to composite a first plurality of rendered 2D elements into an output image, the rendered 2D elements and the plurality of composite nodes corresponding to a first eye of a 3D image; and receiving user input to add one or more transition nodes to the composite nodes, each transition node following a respective composite node and to be used for regeneration of one or more elements corresponding to the respective composite node, wherein the composite nodes and the one or more transition nodes are to be used to direct generation of a second eye of the 3D image.

Abstract: Systems and methods are provided for viewing and displaying 3D imagery. In one implementation, a method of displaying 3D imagery comprises receiving layout data comprising data representing a first camera, the first camera being non-editable; generating a first pair of a left camera and a right camera from the layout data based on the first camera, wherein the left camera and the right camera are editable; retrieving, from an external memory, streaming geometry data corresponding to the layout data; and generating the 3D imagery using the first pair of the left camera and the right camera and the streaming geometry data.

Abstract: Method and systems are provided using streaming geometry in 3D imagery production. In one implementation a method for use in the display of 3D video images comprises receiving a first set of data corresponding to an output from a layout in a production pipeline, generating geometry data for one or more objects represented within the first set of data, merging the geometry data for each of the one or more objects to create one or more merged files, generating a table of contents, wherein the table of contents specifies the location of the geometry data for each of the one or more objects within the one or more merged files and storing the geometry data and the table of contents to a memory device for subsequent retrieval.

Abstract: A developer material holding apparatus holds a developer. A body holds a developer material and includes a first opening through which the developer material is discharged. A shutter is assembled to the body, and is movable either to an opening position where the developer material is discharged from the body or to a closing position where the developer material is not discharged from the body. The shutter includes a second opening such that when the shutter is at the opening position, the second opening is in alignment with the first opening. At least one of the first opening and the second opening is in the form a mesh including a plurality of openings.

Abstract: Several embodiments provide methods and apparatuses for use with node-based compositing to assist in the generation of a second eye or camera of a stereoscopic image. In one implementation, a method for use in 3D movie production comprises the steps: displaying composite nodes to a user, the composite nodes indicating how to composite a first plurality of rendered 2D elements into an output image, the rendered 2D elements and the plurality of composite nodes corresponding to a first eye of a 3D image; and receiving user input to add one or more transition nodes to the composite nodes, each transition node following a respective composite node and to be used for regeneration of one or more elements corresponding to the respective composite node, wherein the composite nodes and the one or more transition nodes are to be used to direct generation of a second eye of the 3D image.

Abstract: Method and systems are provided using streaming geometry in 3D imagery production.

Abstract: Systems and methods are provided for viewing and displaying 3D imagery. In one implementation, a method of displaying 3D imagery comprises receiving layout data comprising data representing a first camera, the first camera being non-editable; generating a first pair of a left camera and a right camera from the layout data based on the first camera, wherein the left camera and the right camera are editable; retrieving, from an external memory, streaming geometry data corresponding to the layout data; and generating the 3D imagery using the first pair of the left camera and the right camera and the streaming geometry data.

Abstract: A developer material holding apparatus holds a developer. A body holds a developer material and includes a first opening through which the developer material is discharged. A shutter is assembled to the body, and is movable either to an opening position where the developer material is discharged from the body or to a closing position where the developer material is not discharged from the body. The shutter includes a second opening such that when the shutter is at the opening position, the second opening is in alignment with the first opening. At least one of the first opening and the second opening is in the form a mesh including a plurality of openings.

Abstract: This invention relates to compounds of formula (I) which are glyoxalase I inhibitors, pharmaceutical salts or compositions comprising such compounds, and the use of such compositions and compounds to treat various conditions alleviated by the inhibition of glyoxalase 1. Wherein X is N or CH. R2 is H, CF3; or optionally substituted C5-6 aryl, C3-7 cycloalkyl, C5-7 heterocyclyl. R3 is H; or optionally substituted C5-6 aryl, C3-7 cycloalkyl, C5-7 heterocyclyl. Alternatively R2 and R3 together form an optionally substituted C3-4alkylene group wherein L3 and L4 are single bonds thus forming a C5-6 ring fused with the aromatic ring to which L3 and L4 are attached. L3 and L4 are independently selected from a single bond, optionally substituted C1-4 alkylene, -L9YN(OH)C(?O)L10- and -L9C(?O)N(OH)YL10-, wherein L9 and L10 are independently selected from optionally substituted C1-4 alkylene, C5-6 arylene, C1-4 alkylene-C5-6arylene and a single bond, wherein Y is NH or a single bond.

Abstract: Compounds of formula (IA) and (IB) are inhibitors of histone deacetylase activity and useful for the treatment of, inter alia, cancers: wherein fused rings A1 and A2 are optionally substituted; linker radical R1 represents a radical of formula

Abstract: The present invention provides a method for enhancing the proliferation and/or hematopoietic differentiation and/or maintenance of mammalian stem cells. The method is useful for generating expanded populations of hematopoietic stem cells (HSCs) and thus mature blood cell lineages. This is desirable where a mammal has suffered a decrease in hematopoietic or mature blood cells as a consequence of disease, radiation or chemotherapy. The method of the present invention comprises increasing the intracellular level of a cdx in stem cells, including hematopoietic stem cells, in culture, either by providing an exogenous cdx protein to the cell, or by introduction into the cell of a genetic construct encoding a cdx. The cdx is selected from the cdx family and includes cdx1, cdx2, or cdx4. The cdx may be a wild type protein appropriate for the species from which the cells are derived, or a mutant form of the protein.