Abstract: The present disclosure relates to a method for reducing quantification errors caused by reaction volume deviations in digital polymerase chain reaction (dPCR) and to a method for determining the amount or concentration of a nucleic acid of interest in a sample with dPCR.

Abstract: The present disclosure relates to methods for determining the amount or concentration of a nucleic acid of interest in an unprocessed sample by analyzing a processed sample and a reference sample with digital polymerase chain reaction (dPCR).

Abstract: A light source module for use in an analytical instrument for analyzing at least one sample is disclosed. The light source module includes at least one light-emitting diode and at least one light guiding rod adapted to guide and shape light emitted by the light-emitting diode. The light source module further includes at least one memory device. The memory device has stored therein at least one driving parameter set, for driving the light-emitting diode in such a way that desired emission properties of light provided by the light source module are generated.

Abstract: The present disclosure provides an instrument and methods for detecting an analyte, comprising a light source capable of generating excitation light for exciting a plurality of luminescence labels, an excitation beam path extending between said light source and said analyte, a detector capable of detecting light emitted from said luminescence label, an emission beam path extending between said analyte and said detector, a filter carrier carrying two or more pairs of filter portions, each pair being related to one luminescence label and comprising a first filter portion for transmitting excitation light and a second filter portion for transmitting emitted light, wherein said first filter portion of one pair is said second filter portion of another pair, and wherein said filter portions are arranged in a manner that a respective one of said pairs can be brought in an operative condition in which said first filter portion is in said excitation beam path and said second filter portion is in said emission beam pat

Abstract: The present disclosure relates to methods for determining the amount or concentration of a nucleic acid of interest in an unprocessed sample by analyzing a processed sample and a reference sample with digital polymerase chain reaction (dPCR).

Abstract: The present disclosure relates to a method for reducing quantification errors caused by reaction volume deviations in digital polymerase chain reaction (dPCR) and to a method for determining the amount or concentration of a nucleic acid of interest in a sample with dPCR.

Abstract: The present disclosure provides an instrument and methods for detecting an analyte, comprising a light source capable of generating excitation light for exciting a plurality of luminescence labels, an excitation beam path extending between said light source and said analyte, a detector capable of detecting light emitted from said luminescence label, an emission beam path extending between said analyte and said detector, a filter carrier carrying two or more pairs of filter portions, each pair being related to one luminescence label and comprising a first filter portion for transmitting excitation light and a second filter portion for transmitting emitted light, wherein said first filter portion of one pair is said second filter portion of another pair, and wherein said filter portions are arranged in a manner that a respective one of said pairs can be brought in an operative condition in which said first filter portion is in said excitation beam path and said second filter portion is in said emission beam pat

Abstract: An instrument is disclosed with a lens system including an objective lens system. The objective lens system is disposed between a light source and the plurality of reaction regions. The objective lens system includes a field lens array, and a pupil plane, wherein the pupil plane and the light source are located on opposite sides of the field lens array.

Abstract: The present disclosure provides instruments and methods for detecting an analyte which are capable of exciting a plurality of luminescence labels and detecting light emitted therefrom. The instrument includes a filter carrier adapted for carrying a plurality of filter portion pairs, each pair related to a luminescence label and comprising a first filter portion for transmitting excitation light, and a second filter portion for transmitting emitted light. The first filter portion of a pair comprises a second filter portion of another pair. Also, the filter portions are arranged such that a pair can be brought into an operative condition whereby a first filter portion is in the excitation beam path and a second filter portion is in the emission beam path. The filter carrier and beam paths may be moved with respect to each other by a moving mechanism so as to bring a pair into operative condition.

Abstract: An analytical processing and detection device is described which comprises a rack capable of holding at least one reaction receptacle, at least one magnetic unit for exerting a magnetic field on at least one reaction receptacle comprising magnetic particles in a fluid, wherein said at least one magnetic unit is reversibly or irreversibly connected to said rack, and wherein said magnetic field causes the magnetic particles to be sequestered to the side walls of said at least one reaction receptacle, and a detection unit for detecting a signal in the fluid in said reaction receptacle.

Abstract: A light source module for use in an analytical instrument for analyzing at least one sample is disclosed. The light source module includes at least one light-emitting diode and at least one light guiding rod adapted to guide and shape light emitted by the light-emitting diode. The light source module further includes at least one memory device. The memory device has stored therein at least one driving parameter set, for driving the light-emitting diode in such a way that desired emission properties of light provided by the light source module are generated.

Abstract: An instrument is disclosed with a lens system including an objective lens system. The objective lens system is disposed between a light source and the plurality of reaction regions. The objective lens system includes a field lens array, and a pupil plane, wherein the pupil plane and the light source are located on opposite sides of the field lens array.

Abstract: The present disclosure provides instruments and methods for detecting an analyte which are capable of exciting a plurality of luminescence labels and detecting light emitted therefrom. The instrument includes a filter carrier adapted for carrying a plurality of filter portion pairs, each pair related to a luminescence label and comprising a first filter portion for transmitting excitation light, and a second filter portion for transmitting emitted light. The first filter portion of a pair comprises a second filter portion of another pair. Also, the filter portions are arranged such that a pair can be brought into an operative condition whereby a first filter portion is in the excitation beam path and a second filter portion is in the emission beam path. The filter carrier and beam paths may be moved with respect to each other by a moving mechanism so as to bring a pair into operative condition.

Abstract: The invention concerns an optical instrument for imaging fluorescence signals from an arrangement of a plurality of individual detection sites, for example the wells of a microtitre plate. In order to improve the light yield of the fluorescence excitation with excitation light as well as the light yield of the detection of the fluorescence signals, an objective array is provided which is arranged in the beam path between the field lens and the detection sites and comprises a field lens array with field lens array elements and a pupil lens array with pupil lens array elements. In order to improve the channel separation and suppress interfering light the objective array can comprise a diaphragm array with in each case two diaphragm openings per detection site.

Abstract: An analytical processing and detection device is described which comprises a rack capable of holding at least one reaction receptacle, at least one magnetic unit for exerting a magnetic field on at least one reaction receptacle comprising magnetic particles in a fluid, wherein said at least one magnetic unit is reversibly or irreversibly connected to said rack, and wherein said magnetic field causes the magnetic particles to be sequestered to the side walls of said at least one reaction receptacle, and a detection unit for detecting a signal in the fluid in said reaction receptacle.

Abstract: The present invention relates to a system for the optical detection of light from analytical samples, the system comprising an analytical instrument comprising an optical detection unit and a sample block unit for receiving a plurality of analytical samples, the system further comprising a calibration device for calibrating the optical detection unit of the analytical instrument, wherein the calibration device comprises a substrate and at least one electrically powered reference light source coupled to the substrate, said at least one reference light source emitting light being detected by the optical detection unit.

Abstract: The invention concerns an optical instrument for imaging fluorescence signals from an arrangement of a plurality of individual detection sites, for example the wells of a microtitre plate. In order to improve the light yield of the fluorescence excitation with excitation light as well as the light yield of the detection of the fluorescence signals, an objective array is provided which is arranged in the beam path between the field lens and the detection sites and comprises a field lens array with field lens array elements and a pupil lens array with pupil lens array elements. In order to improve the channel separation and suppress interfering light the objective array can comprise a diaphragm array with in each case two diaphragm openings per detection site.