Abstract: Materials and methods for conducting an atom transfer radical polymerization in the presence of oxygen by interlocking enzymatic activities are provided herein.

Abstract: Materials and methods for conducting an atom transfer radical polymerization in the presence of oxygen by interlocking enzymatic activities are provided herein.

Abstract: Materials and methods for conducting an atom transfer radical polymerization in the presence of oxygen by interlocking enzymatic activities are provided herein.

Abstract: Materials and methods for protein purification, and particularly for protein purification by ammonium sulfate precipitation, are provided herein.

Abstract: Provided herein are highly porous electrode structures made from natural materials and retaining natural architecture, which are highly permeable and find use in capacitive deionization desalination systems and methods. Also provided herein are methods of making the electrodes and desalination systems, as well as methods of desalinating water.

Abstract: Methods and materials for generating and using complexes that contain erythrocytes coupled to polypeptides, and particularly to erythrocyte-polypeptide complexes that can interact with biological molecules, are provided herein.

Abstract: This document relates to materials and methods for using controlled radical polymerization (e.g., atom transfer radical polymerization) to generate protein-polymer conjugates in which two or more polymer molecules are attached to individual initiator molecules on the protein.

Abstract: Provided herein are materials and methods that include utilizing atom transfer radical polymerization (ATRP) initiator molecules that maintain a positive charge during biomacro-initiator synthesis.

Abstract: Materials and methods for targeted and controlled addition of polymers to proteins are provided herein. For example, the methods provided herein can include contacting a protein with (i) an inactive controlled radical polymerization (CRP) initiator blocker that can reacts with amino groups on the protein but cannot serve as an initiator for polymer addition, and (ii) an active CRP initiator that reacts with amino groups on the protein and also includes a group through which a polymer can be coupled to the initiator.

Abstract: Embodiments of the invention provide at least one polymer covalently conjugated to an esterase. The at least one polymer includes a plurality of oxime functional groups.

Abstract: Embodiments of the invention provide at least one polymer covalently conjugated to an esterase. The at least one polymer includes a plurality of oxime functional groups.

Abstract: Materials and methods for generating protein-polymer conjugates on solid supports are provided herein. The methods can include, for example, reversibly immobilizing a protein on a solid support, modifying the protein by adding polymer subunits, and releasing the protein-polymer conjugate from the solid support.

Abstract: Materials and methods for conducting an atom transfer radical polymerization in the presence of oxygen by interlocking enzymatic activities are provided herein.

Abstract: Embodiments of the invention provide at least one polymer covalently conjugated to an esterase. The at least one polymer includes a plurality of oxime functional groups.

Abstract: Embodiments of the invention provide at least one polymer covalently conjugated to an esterase. The at least one polymer includes a plurality of oxime functional groups.

Abstract: Provided herein are protein-polymer conjugates, pharmaceutical compositions including protein-polymer conjugates, and methods of using the same, e.g., in therapeutic and industrial applications.

Abstract: Embodiments of the invention provide at least one polymer covalently conjugated to an esterase. The at least one polymer includes a plurality of oxime functional groups.

Abstract: An enzyme-polymer conjugate shows increased activity and molecular dissolution in non-aqueous solvents enabling enzyme mediated catalysis in non-aqueous solutions. The inventions described in this specification relate to enzyme-polymer conjugates, organic solutions comprising enzyme-polymer conjugates, methods of dissolving enzyme-polymer conjugates in organic solvents, and methods of using enzyme-polymer conjugates, for example, in catalysis applications. The inventions described in this specification address the problems of insolubility and decreased activity of enzymes in non-aqueous solutions comprising, for example, organic solvents or ionic liquids.

Abstract: Using a novel water-soluble, active ester amide-containing functionalized controlled radical polymerization initiator, stimuli responsive polymers have been grown from the surface of a protein, exemplified by chymotrypsin or any protein having surface amino acids that will covalently bind to the active ester amide-containing functionalized initiator. It is shown that changes in temperature or pH can change the conformation of the polymer surrounding the enzyme, which in turn enabled the rational tailoring of enzyme activity and stability. This method has afforded an increase in the activity and stability of the enzyme by an order of magnitude at pH's where the enzyme is usually inactive or unstable. Multimodal temperature responsive protein-block copolymer conjugates are described.

Abstract: Embodiments of the invention provide at least one polymer covalently conjugated to an esterase. The at least one polymer includes a plurality of oxime functional groups.