Patents by Inventor Alan L. Greener
Alan L. Greener has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 7148339Abstract: The subject invention provides for a method of selectively cloning homoduplex nucleic acid molecules, in particular, by using a strain of host cells that contains a conditionally expressed and/or conditionally active mismatch-recognizing enzyme, e.g., a temperature sensitive variant of the gene encoding the endonuclease VII from phage T4. Using this host strain, the invention features a novel cloning method that selects for PCR products that are devoid of PCR-generated mutations.Type: GrantFiled: March 23, 2005Date of Patent: December 12, 2006Assignee: Stratagene CaliforniaInventors: Alan L. Greener, Lisa Joy Hexdall, Carsten Peter Carstens, Joseph A. Sorge
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Patent number: 6869797Abstract: This invention provides improved electroporation methods for transferring nucleic acids of interest into host cells, wherein the host cells are (1) suspended in a substantially non-ionic solution comprising at least one sugar or sugar derivative, (2) mixed with the nucleic acids of interest, and (3) electrically treated. Also, this invention provides for kits used in the method for transferring nucleic acids into host cells.Type: GrantFiled: June 30, 2003Date of Patent: March 22, 2005Assignee: Stratagene CaliforniaInventors: Alan L. Greener, Bruce D. Jerpseth
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Publication number: 20040209362Abstract: Provided are methods of generating electrocompetent bacterial cells, the methods involving the growth of the cells at hyperosmotic salt concentration. Also provided are methods of producing a transformed cell, methods of producing a recombinant polypeptide, and strains of E. coli that exhibit increased electrotransformation efficiency.Type: ApplicationFiled: March 19, 2004Publication date: October 21, 2004Applicant: StratageneInventor: Alan L. Greener
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Publication number: 20040137632Abstract: This invention provides improved electroporation methods for transferring nucleic acids of interest into host cells, wherein the host cells are (1) suspended in a substantially non-ionic solution comprising at least one sugar or sugar derivative, (2) mixed with the nucleic acids of interest, and (3) electrically treated. Also, this invention provides for kits used in the method for transferring nucleic acids into host cells.Type: ApplicationFiled: June 30, 2003Publication date: July 15, 2004Inventors: Alan L. Greener, Bruce D. Jerpseth
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Publication number: 20040137628Abstract: This invention provides methods for producing and selecting host cells that better survive transformation treatment by subjecting host cells to conditions that alter them, subjecting the altered cells to transformation conditions, and selecting host cells that survive the transformation conditions. This invention also provides methods for transferring nucleic acids of interest into host cells, using cells that are better able to survive transformation treatment. Also, this invention provides kits for producing or selecting host cells in transformation treatments, as well as, kits comprising various host cells that may be utilized in transformation experiments.Type: ApplicationFiled: October 14, 2003Publication date: July 15, 2004Applicant: StratageneInventor: Alan L. Greener
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Publication number: 20040002155Abstract: The subject invention provides for a strain of host cells that contains a temperature sensitive variant of the gene encoding the endonuclease VII from phage T4. Using this host strain, the invention features a novel cloning method that selects for PCR products that are devoid of PCR-generated mutations.Type: ApplicationFiled: June 26, 2002Publication date: January 1, 2004Applicant: StratageneInventors: Alan L. Greener, Lisa Joy Hexdall, Carsten-Peter Carstens, Joseph A. Sorge
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Publication number: 20040002156Abstract: The subject invention provides for a method of selectively cloning homoduplex nucleic acid molecules, in particular, by using a strain of host cells that contains a conditionally expressed and/or conditionally active mismatch-recognizing enzyme, e.g., a temperature sensitive variant of the gene encoding the endonuclease VII from phage T4. Using this host strain, the invention features a novel cloning method that selects for PCR products that are devoid of PCR-generated mutations.Type: ApplicationFiled: September 19, 2002Publication date: January 1, 2004Applicant: StratageneInventors: Alan L. Greener, Lisa Joy Hexdall, Carsten-Peter Carstens, Joseph A. Sorge
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Patent number: 6635457Abstract: This invention provides methods for producing and selecting host cells that better survive transformation treatment by subjecting host cells to conditions that alter them, subjecting the altered cells to transformation conditions, and selecting host cells that survive the transformation conditions. This invention also provides methods for transferring nucleic acids of interest into host cells, using cells that are better able to survive transformation treatment. Also, this invention provides kits for producing or selecting host cells in transformation treatments, as well as, kits comprising various host cells that may be utilized in transformation experiments.Type: GrantFiled: July 26, 2000Date of Patent: October 21, 2003Assignee: StratageneInventor: Alan L. Greener
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Patent number: 6586249Abstract: This invention provides improved electroporation methods for transferring nucleic acids of interest into host cells, wherein the host cells are (1) suspended in a substantially non-ionic solution comprising at least one sugar or sugar derivative, (2) mixed with the nucleic acids of interest, and (3) electrically treated. Also, this invention provides for kits used in the method for transferring nucleic acids into host cells.Type: GrantFiled: January 15, 2002Date of Patent: July 1, 2003Assignee: StratageneInventors: Alan L. Greener, Bruce D. Jerpseth
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Publication number: 20030096223Abstract: The present invention relates to methods for the identification of polynucleotides that encode cleavable N-terminal signal sequences.Type: ApplicationFiled: February 28, 2001Publication date: May 22, 2003Inventors: Alan L. Greener, Hwai Wen Chang, Carsten Peter Carstens
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Publication number: 20020164804Abstract: This invention provides improved electroporation methods for transferring nucleic acids of interest into host cells, wherein the host cells are (1) suspended in a substantially non-ionic solution comprising at least one sugar or sugar derivative, (2) mixed with the nucleic acids of interest, and (3) electrically treated. Also, this invention provides for kits used in the method for transferring nucleic acids into host cells.Type: ApplicationFiled: January 15, 2002Publication date: November 7, 2002Applicant: StratageneInventors: Alan L. Greener, Bruce D. Jerpseth
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Patent number: 6338965Abstract: This invention provides improved electroporation methods for transferring nucleic acids of interest into host cells, wherein the host cells are (1) suspended in a substantially non-ionic solution comprising at least one sugar or sugar derivative, (2) mixed with the nucleic acids of interest, and (3) electrically treated. Also, this invention provides for kits used in the method for transferring nucleic acids into host cells.Type: GrantFiled: March 17, 2000Date of Patent: January 15, 2002Assignee: StratageneInventors: Alan L. Greener, Bruce D. Jerpseth
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Patent number: 6040184Abstract: This invention provides improved electroporation methods for transferring nucleic acids of interest into host cells, wherein the host cells are (1) suspended in a substantially non-ionic solution comprising at least one sugar or sugar derivative, (2) mixed with the nucleic acids of interest, and (3) electrically treated. Also, this invention provides for kits used in the method for transferring nucleic acids into host cells.Type: GrantFiled: February 22, 1999Date of Patent: March 21, 2000Assignee: StratageneInventors: Alan L. Greener, Bruce D. Jerpseth
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Patent number: 6017748Abstract: Methods and materials for the cloning of DNA, in particular, for the cloning of "unclonable" DNA using genetically engineered host cells. Host cell organisms have been discovered that stabilize and inhibit rearrangement of DNA molecules capable of forming non-standard secondary and tertiary structures. organisms are engineered to contain at least one mutation which inactivates homologous recombination and at least one mutation in a DNA repair pathway. Examples of such DNA pathways include UV repair pathway, the SOS repair pathway, the mismatch repair pathway, the adaptive response pathway, the heat shock response pathway, the osmotic shock response pathway, the repair pathway of alkylation damage, the repair pathway of uracil incorporation into DNA and pathways involved in maintaining DNA superhelicity. The host organisms of this invention are suitable for cloning DNA capable of forming non-standard and tertiary structures such as found in eukaryotic DNA.Type: GrantFiled: June 7, 1995Date of Patent: January 25, 2000Assignee: StratageneInventor: Alan L. Greener
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Patent number: 5707841Abstract: The invention provided herein includes gram negative bacteria cells containing a gene encoding an enzyme with carbohydrate degrading activity that had been rendered competent to transformation. Carbohydrate degrading enzymes of interest for use in the invention include alpha-amylase. The competent cells of the subject invention may be frozen so as to provide for prolonged storage. Other aspects of the invention include methods for rendering gram negative bacterial cells, such as E. coli cells competent to transformation. These methods involve the step of transferring a gene encoding an enzyme with carbohydrate degrading activity into E. coli cells and subsequently rendering the cells competent using any of a variety of competency inducing procedures.Type: GrantFiled: April 18, 1996Date of Patent: January 13, 1998Assignee: StratageneInventor: Alan L. Greener
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Patent number: 5552314Abstract: Methods and materials for the cloning of DNA, in particular, for the cloning of "unclonable" DNA using genetically engineered host cells. Host cell organisms have been discovered that stabilize and inhibit rearrangement of DNA molecules capable of forming non-standard secondary and tertiary structures. Organisms are engineered to contain at least one mutation which inactivates homologous recombination and at least one mutation in a DNA repair pathway. Examples of such DNA pathways include UV repair pathway, the SOS repair pathway, the mismatch repair pathway, the adaptive response pathway, the heat shock response pathway, the osmotic shock response pathway, the repair pathway of alkylation damage, the repair pathway of uracil incorporation into DNA and pathways involved in maintaining DNA superhelicity. The host organisms of this invention are suitable for cloning DNA capable of forming non-standard and tertiary structures such as found in eukaryotic DNA.Type: GrantFiled: May 15, 1995Date of Patent: September 3, 1996Assignee: StratageneInventor: Alan L. Greener
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Patent number: 5512468Abstract: The invention provided herein includes gram negative bacteria cells containing a gene encoding an enzyme with carbohydrate degrading activity that had been rendered competent to transformation. Carbohydrate degrading enzymes of interest for use in the invention include alpha-amylase. The competent cells of the subject invention may be frozen so as to provide for prolonged storage.Other aspects of the invention include methods for rendering gram negative bacterial cells, such as E. coli cells competent to transformation. These methods involve the step of transferring a gene encoding an enzyme with carbohydrate degrading activity into E. coli cells and subsequently rendering the cells competent using any of a variety of competency inducing procedures.Type: GrantFiled: November 22, 1993Date of Patent: April 30, 1996Assignee: StratageneInventor: Alan L. Greener