Abstract: The present invention relates to the provision of novel medicaments for the treatment, prevention or amelioration of allergic disease. In particular, the novel medicaments are isolated peptides incorporating epitopes or mimotopes of surface exposed regions of the C?2 domain of IgE. The inventors have found that these novel regions may be the target for both passive and active immunoprophylaxis or immunotherapy. The invention further relates to methods for production of the medicaments, pharmaceutical compositions containing them and their use in medicine. Also forming an aspect of the present invention are ligands, especially monoclonal antibodies, which are capable of binding the surface exposed IgE regions of the present invention, and their use in medicine as passive immunotherapy or in immunoprophylaxis.

Abstract: This invention provides for the active site mapping of enzymes which catalyse covalent modification including, but not limited to phosphorylation, acylation, dephosphorylation in which a fixed residue (known as the catalytic residue) such as a tyrosine, serine, threonine, histidine, aspartic acid residue or any other residue containing an appropriate side chain is modified. Mapping of protein kinases is exemplified. The method of the invention has an additional level of complexity over and above that of the self-deconvoluting libraries described in W097/42216. This involves making a library of smaller libraries (referred to as subsets) where a fixed residue is moved stepwise through the sequence of amino acids or other groups (such as peptidomimetics). Using 5 subsets of libraries of peptides of 5 amino acids allows the mapping of a sequence of 9 amino acids.