Abstract: Systems and methods for determining a concentration of an analyte in a physiological fluid with a biosensor are presented. Current values are measured during application of voltage pulses across electrodes of the biosensor. Different intermediate analyte concentrations are calculated using different subsets of the measured current values and different scaling factors. A first intermediate analyte concentration has a first level of accuracy across a range of analyte concentrations. A second intermediate analyte concentration has a higher level of accuracy in the low range. A third intermediate analyte concentration has a higher level of accuracy in the high range. The concentration of the analyte is determined as a function of the different intermediate analyte concentrations. The second intermediate analyte concentration, the third intermediate analyte concentration or an average, is selected if the first intermediate analyte concentration is in the low range, the high range or in between, respectively.

Abstract: A method of measuring a sample that includes at least one reactant that can be oxidised and reduced between at least one working electrode and a counter electrode. The method involves: applying across the working and counter electrodes a cycle of at least three pulses, and measuring current at the working electrode during each pulse, wherein the at least three pulses comprise at least one over-potential pulse that has an amplitude equal to or greater than an oxidation or a reduction peak potential of the reactant; at least one under-potential pulse of amplitude less than the at least one over-potential pulse, and at least one other over-potential pulse or under-potential pulse.

Abstract: The invention provides an enzyme ink useful in test strips that provides a test strip bias, at the low and high glucose ends, falling within a desired target range. The ink of the invention permits an improved method for the production of single calibration code strip lots with good yields.

Abstract: An electrochemical-based analytical test strip for the determination of an analyte (such as glucose) in a bodily fluid sample (e.g., a whole blood sample) includes an electrically-insulating substrate, at least one working electrode disposed on the electrically-insulating substrate and a graded enzymatic reagent layer disposed on the at least one working electrode. The graded enzymatic reagent layer includes an upper reaction grade that contains an enzyme. The graded enzymatic layer also has a lower spacer grade devoid of the enzyme, the lower spacer grade disposed between the upper reaction grade and the working electrode such that the upper reaction grade is spaced equidistant from the working electrode by the lower spacer grade by a predetermined distance during use of the electrochemical-based analytical test strip.

Abstract: The invention provides an enzyme ink useful in test strips that provides a test strip bias, at the low and high glucose ends, falling within a desired target range. The ink of the invention permits an improved method for the production of single calibration code strip lots with good yields.

Abstract: A fusible conductive ink for use in manufacturing microfluidic analytical systems includes micronised powder containing platinum and carbon, poly(bisphenol A-co-epichlorohydrin)-glycidyl end capped polymer, and a solvent. In addition, the ratio of micronised powder to poly(bisphenol A-co-epichlorohydrin)-glycidyl end capped polymer is in the range of 3:1 to 1:3. The fusible conductive inks can be employed in the manufacturing of microfluidic systems to form electrodes, electrically conductive traces and/or electrically conductive contact pads.

Abstract: An enzymatic electrochemical-based sensor includes a substrate and a conductive layer formed from a dried water-miscible conductive ink. The water-miscible conductive ink used to form the conductive layer includes a conductive material, an enzyme, a mediator, and a binding agent and is formulated such that the water-miscible conductive ink is a water-miscible aqueous-based dispersion and the binding agent became operatively water-insoluble upon drying.

Abstract: A method for manufacturing a portion of an enzymatic electrochemical-based sensor includes applying a water-miscible conductive ink to a substrate of an enzymatic electrochemical-based sensor. The water-miscible conductive ink includes a conductive material, an enzyme, a mediator, and a binding agent (that becomes operatively water-insoluble upon drying) formulated as a water-miscible aqueous-based dispersion. The method further includes the drying the water-miscible conductive ink to form a conductive layer on the substrate that includes an operatively water-insoluble binding agent.

Abstract: A water-miscible conductive ink for use in enzymatic electrochemical-based sensors includes a conductive material, an enzyme, a mediator and a binding agent. The conductive material, enzyme, mediator, and binding agent are formulated as a water-miscible aqueous-based dispersion wherein the binding agent becomes operatively water-insoluble upon drying.

Abstract: A fusible conductive ink for use in manufacturing microfluidic analytical systems includes micronised powder containing platinum and carbon, poly(bisphenol A-co-epichlorohydrin)-glycidyl end capped polymer, and a solvent. In addition, the ratio of micronised powder to poly(bisphenol A-co-epichlorohydrin)-glycidyl end capped polymer is in the range of 3:1 to 1:3. The fusible conductive inks can be employed in the manufacturing of microfluidic systems to form electrodes, electrically conductive traces and/or electrically conductive contact pads.

Abstract: A method for manufacturing an analysis module with accessible electrically conductive contact pads includes forming an insulating substrate with an upper surface, a microchannel(s) within the upper surface, and electrically conductive contact pad(s) disposed on the upper surface. The method also includes producing a laminate layer with a bottom surface, electrode(s) on the laminate layer bottom surface, and electrically conductive trace(s) on the laminate layer bottom surface. The method further includes adhering the laminate layer to the insulating substrate such that a portion of the bottom surface of the laminate layer is adhered to a portion of the upper surface of the insulating substrate, each electrode is exposed to at least one microchannel; and each electrically conductive trace is electrically contacted to at least one electrically conductive contact pad.

Abstract: A microfluidic analytical system for monitoring an analyte (such as glucose) in a fluid sample (e.g., blood or ISF) includes an analysis module and an electrical device (for example, a meter or power supply). The analysis module includes an insulating substrate and a microchannel(s) within the insulating substrate's upper surface. The analysis module also includes a conductive contact pad(s) disposed on the upper surface of the insulating substrate and an electrode(s), with the electrode(s) being disposed over the microchannel. In addition, the analysis module includes an electrically conductive trace(s) that electrically connects the electrode to at least one electrically conductive contact pad. The analysis module also has a laminate layer disposed over the electrode, the electrically conductive trace, the microchannel and a portion of the upper surface of the insulating substrate.

Abstract: A microfluidic analytical system for monitoring an analyte (for example, glucose) in a liquid sample (e.g., ISF) includes an analysis module with at least one micro-channel for receiving and transporting a liquid sample, at least one analyte sensor for measuring an analyte in the liquid sample and at least one position electrode. The analyte sensor(s) and position electrode(s) are in operative communication with the micro-channel. The microfluidic system also includes a meter configured for measuring an electrical characteristic (such as impedance or resistance) of the position electrode(s). Moreover, the measured electrical characteristic is dependent on the position of the liquid sample in the micro-channel that is in operative communication with the position electrode for which an electrical characteristic is measured.

Abstract: Devices and kits for modulating the flow of a liquid are provided.

Abstract: Methods for modulating the flow of a liquid are provided.

Abstract: The invention described is a method of segregating a bolus of fluid using a pneumatic actuator in a fluid handling circuit. The described invention further includes a method of segregating a bolus of fluid using a pneumatic actuator in a fluid handling circuit wherein the method includes the step of injecting an air pocket into the fluid stream to create the bolus. In addition, the described invention includes a method of measuring the analyte concentration in a bolus of fluid using a pneumatic actuator in a fluid handling circuit. The described invention further includes a method of measuring the analyte concentration in a bolus of fluid using a pneumatic actuator in a fluid handling circuit wherein the method includes the step of injecting an air pocket into the fluid stream to create the bolus.

Abstract: The invention described herein includes a pneumatic actuator for bolus generation in a fluid handling circuit and a pneumatic actuator for bolus generation in a fluid handling circuit wherein the pneumatic actuator is used to insert an air pocket into the fluid stream to create the bolus. Further, The invention described herein includes a pneumatic fluid handling circuit including a pneumatic actuator for bolus generation including a pneumatic fluid handling circuit including a pneumatic actuator for bolus generation wherein the pneumatic actuator is used to insert an air pocket into the fluid stream to create the bolus.

Abstract: A microfluidic analytical system for monitoring an analyte (for example, glucose) in a liquid sample (e.g., ISF) includes an analysis module with at least one micro-channel for receiving and transporting a liquid sample, at least one analyte sensor for measuring an analyte in the liquid sample and at least one position electrode. The analyte sensor(s) and position electrode(s) are in operative communication with the micro-channel. The microfluidic system also includes a meter configured for measuring an electrical characteristic (such as impedance or resistance) of the position electrode(s). Moreover, the measured electrical characteristic is dependent on the position of the liquid sample in the micro-channel that is in operative communication with the position electrode for which an electrical characteristic is measured.

Abstract: The invention relates to a paste, which can undergo screen printing, for producing a porous polymer membrane. Said paste contains at least one polymer, one or more solvents for the polymer having a boiling point of >100° C., one or more non-solvents for the polymers (pore-forming agents) having a higher boiling point than that of the solvent(s), and contains a hydrophilic viscosity modifier.

Abstract: The invention relates to a paste, which can undergo screen printing, for producing a porous polymer membrane. Said paste contains at least one polymer, one or more solvents for the polymer having a boiling point of >100° C., one or more non-solvents for the polymers (pore-forming agents) having a higher boiling point than that of the solvent(s), and contains a hydrophilic viscosity modifier.