Abstract: A method for calibrating a patrol vehicle speed, comprising initiating a calibration cycle at a speed detection radar unit mounted in a vehicle, operating the vehicle to generate a vehicle speed signal using the speed detection radar unit and exiting the calibration cycle if the vehicle speed signal matches an observed speed from an independent source signal.

Abstract: Thermostable viral and microbial polymerases exhibiting a combination of activities selected from proofreading (3?-5?) exonuclease activity, nick translating (5?-3?) nuclease activity, synthetic primer-initiated polymerase activity, nick-initiated polymerase activity, reverse transcriptase activity, strand displacement activity, terminal transferase activity, primase activity, and/or efficient incorporation of chain terminating analogs. Some of the polymerases provided herein include a first motif and a second motif. The first motif preferably has the sequence X1X2X3DX4PX5IELRX6X7X8, wherein X1 is I or V; X4 is F or Y; X8 is G or A; and X2, X3, X5, X6, and X7 are any amino acid. The second motif preferably has the sequence RX9X10X11KSANX12GX13X14YG, wherein X11 is G or A; X12 is F, L, or Y; X13 is L or V; X14 is I or L; and X9 and X10 are any amino acid.

Abstract: An enhanced police Doppler direction sensing radar detects possibly dangerous traffic conditions during certain vehicle maneuvers such as U-turns and returns to travel after roadside stops. By monitoring a host or primary vehicle speed, speed transitions, transmission state (e.g. gear selection), and the closing vehicle position, range and speed, a number of selectable conditions are detected, resulting in an alert indication to a primary vehicle operator. User preferences and thresholds allow the traffic alert function to be customized according to a primary vehicle operator's desire to suppress alerts in situations which the user does not deem dangerous. The traffic alert function may be automatically triggered under certain detected conditions, or manually initiated when the primary vehicle operator intends to make a driving maneuver.

Abstract: A system for processing vehicle speed data for a vehicle is provided. The system includes a front antenna assembly of the vehicle generating a front digital signal, and a rear antenna assembly of the vehicle generating a rear digital signal. A fast Fourier transform system converts the front digital signal into front frequency shift data and the rear digital signal into rear frequency shift data. A patrol speed system matches the front frequency shift data and the rear frequency shift data and generates a vehicle speed for the vehicle.

Abstract: The present invention relates to systems, methods, and compositions for cloning and sequencing insert nucleic acid sequences. In particular, the present invention provides vectors and vector components configured for multiplex cloning, multiplex sequencing, and fixed orientation cloning. The present invention also provides vectors and vector components that allow insert sequences that are deleterious to a host cell to be successfully cloned.

Abstract: The present invention relates to systems, methods, and compositions for cloning and sequencing insert nucleic acid sequences. In particular, the present invention provides vectors and vector components configured for multiplex cloning, multiplex sequencing, and fixed orientation cloning. The present invention also provides vectors and vector components that allow insert sequences that are deleterious to a host cell to be successfully cloned.

Abstract: The present invention relates to systems, methods, and compositions for cloning and sequencing insert nucleic acid sequences. In particular, the present invention provides vectors and vector components configured for multiplex cloning, multiplex sequencing, and fixed orientation cloning. The present invention also provides vectors and vector components that allow insert sequences that are deleterious to a host cell to be successfully cloned.

Abstract: The present invention relates to systems, methods, and compositions for cloning and sequencing insert nucleic acid sequences. In particular, the present invention provides vectors and vector components configured for multiplex cloning, multiplex sequencing, and fixed orientation cloning. The present invention also provides vectors and vector components that allow insert sequences that are deleterious to a host cell to be successfully cloned.