Abstract: The present invention is directed to a system and method which measures transcellular parameters in which cells are cultured in a monolayer on a biomaterial. The parameters measured are indicative of in vivo values such as cell-cell and cell-matrix adhesion parameters.

Abstract: Methods for measuring cell membrane property are disclosed. A computer readable medium includes instructions for deriving from transcellular impedance a measurement of a cell membrane property and provides the stability and error of the measurement. The derivation from the transcellular impedance includes the real and imaginary components of the transcellular impedance and also uses a geometric shape to model the shape of the cell. Alternatively, the derivation from the transcellular impedance includes magnitude and complex components. The measurements include the membrane capacitance, membrane resistance, and/or cell adhesion.

Abstract: Embodiments of the invention include compositions and methods for the inhibition of pathogen-mediated cytopathic effects by contacting a cell with an 1-CaD polynucleotide or polypeptide. In still further embodiments, a nucleic acid encoding 1-CaD is administered to cell infected by or at risk of infection by a pathogen. Gene delivery of 1-CaD shows a reduced cell toxicity compared to cytochalasin. The delivery of 1-CaD affords a protection on or therapy for modulating cell membrane integrity for protection against an infection.