Abstract: An animal pregnancy test which includes detecting a hormone level in a bodily extract. The bodily extract is tested for a threshold level of the hormone, specifically oestrogen. The test includes a test substance and a material capable of facilitating movement of bodily extract within it. The test substance includes a substance capable of reacting with oestrone sulphate and an indicator dye which is attached to the surface of the substance capable of reacting with oestrone sulphate. The threshold level of the test substance is calibrated to suit specific species and to indicate the desired litter size of that species so that if the detected level of oesterone sulphate exceeds a predetermined threshold level for that species, then there is a high probability of the actual litter size exceeding the calibrated litter size. The apparatus for detecting pregnancy in animals consists of a testing substance, a control test, a sample extractor, and a sample container.